The Caatinga endemic Manilkara rufula possesses remarkable activity against Trichomonas vaginalis and Tritrichomonas foetus

Vieira, Patrícia de Brum; Silva, Nícolas Luiz Feijó; Silva, Denise Brentan; Lopes, Norberto Peporine; Silva, Alexandre Gomes da; Silva, Márcia Vanusa da; Macêdo, Alexandre José; Tasca, Tiana

doi:10.1016/j.exppara.2016.12.006

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…microphylla had antiproliferative properties on Trichomonas spp. The results of our study further confirmed this previous evidence ( 19 – 20 ).…”

Section: Discussionsupporting

confidence: 93%

Alpha-Pinene and Tannic Acid Inhibit Trichomonas vaginalis Protozoan Cells by Inducing Apoptosis

Moradi,

Dastan,

Fallah

et al. 2024

IJPA

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Background: Trichomoniasis is one of the most common sexually transmitted infections worldwide. The growing concern of drug resistance of this infection has cautioned the need for new drug development. We evaluated the potential antiproliferative and apoptotic effect of α-pinene and tannic acid (TA) on Trichomonas vaginalis cells. In addition, the cytotoxicity of agents on Vero cells was investigated. Methods: Trichomonas cells were axenically cultured in TYI-S-33 medium. In vitro antiproliferative activity of α-pinene, TA, and metronidazole was investigated against Trichomonas cells. The assays were carried out in triplicate using microtiter plate and trypan blue staining method. Annexin V/PI staining with flow cytometry was used to evaluate apoptosis induction. In addition, the cytotoxic effect was measured by MTT assay. Results: α-Pinene and TA exhibited significant inhibition of the Trichomonas cells and the lowest IC50 values were 22.9 µg/ml and 140 µg/ml at 48 hours’ incubation, respectively. The CC50 was found at 116 μg/ml for α-pinene and 473 μg/ml for TA, after 48 hours of treatment. The flow cytometry study demonstrated that the natural compounds induced apoptosis in Trichomonas cells. After 24 hours of treatment, the induction of apoptosis was 5.2% - 36.6% at concentrations of 3.9 - 62.5 μg/ml for α-pinene and TA induced-apoptosis was 6.1% - 53.8% at concentrations of 125-2000 μg/ml. Conclusion: Although the results show the antiproliferative and apoptotic effect of α-pinene and TA on Trichomonas cells, in vivo studies are needed to further clarify the effects of these compounds.

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“…microphylla had antiproliferative properties on Trichomonas spp. The results of our study further confirmed this previous evidence ( 19 – 20 ).…”

Section: Discussionsupporting

confidence: 93%

Alpha-Pinene and Tannic Acid Inhibit Trichomonas vaginalis Protozoan Cells by Inducing Apoptosis

Moradi,

Dastan,

Fallah

et al. 2024

IJPA

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“…S1). The compound was inferred as a procyanidin dimer based on the mass spectra 35,36 . After comparison with the reference standard by HPLC, peak 4 was finally confirmed as procyanidin B2.…”

Section: Resultsmentioning

confidence: 99%

“…S1). 35,37 After comparison with the reference standard by HPLC, peak 7 was finally confirmed as epicatechin gallate.…”

Section: Resultsmentioning

confidence: 99%

Extraction, identification, and starch‐digestion inhibition of phenolics from Euryale ferox seed coat

Zhang¹,

Zeng

Yuan

et al. 2023

J Sci Food Agric

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BACKGROUND: Euryale ferox is an important cash crop and valuable tonic in traditional medicine. The seeds of E. ferox are rich in starch, which is hard to digest, and the digestion speed is significantly slower than that of rice starch. The goal of this study was to evaluate the effects of E. ferox seed-coat phenolics (EFCPs) on the digestion of E. ferox seed starch. RESULTS: EFCPs were extracted and identified by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. We optimized the extraction parameters, and the final extraction yield was about 1.49%. We identified seven phenolics from the E. ferox seed-coat extracts: gallic acid, digalloylhexoside, catechin, procyanidin B2, epicatechin, ellagic acid, and epicatechin gallate. Quantitative analysis results showed that the E. ferox seed phenolics mainly distributed in the seed coat and the gallic acid, digalloylhexoside, and epicatechin gallate were three main phenolic compounds. The phenolics displayed strong inhibitory activities on ⊍-glucosidase and ⊍-amylase with an IC 50 of 3.25 ∼g mL −1 and 1.36 mg mL −1 respectively. Furthermore, these phenolics could interact with starch by hydrogen bonds, which might make its starch more difficult to digest. CONCLUSION: Our investigation suggests that the EFCPs can strongly inhibit the digestion of E. ferox seed starch by inhibiting the ⊍-amylase and ⊍-glucosidase activities and interacting with starch by hydrogen bonds; therefore, E. ferox seeds have a promising application prospect in foods for hypoglycemia.

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“…In a study conducted byde brum Vieira et al (2017) evaluated the anti-T. vaginalis activity of 7 fractions from Manilkara rufula (Miq.) H.J.Lam leaves extracted with acetone and analyze by LC-DAD-MS/MS (liquid chromatography-diode array detectortandem mass spectrometry), MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry), and MALDI-MS/MS.…”

mentioning

confidence: 99%

Bioactive Compounds as Potential Agents for Sexually Transmitted Diseases Management: A Review to Explore Molecular Mechanisms of Action

et al. 2021

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Sexually transmitted diseases (STDs) are produced by pathogens like bacteria, fungi, parasites, and viruses, and may generate severe health problems such as cancer, ulcers, and even problems in the newborn. This narrative review aims to present updated information about the use of natural bioactive compounds for the prevention and treatment of sexually transmitted infections. A search of the literature was performed using databases and search engines such as PubMed, Scopus, Google Scholar and Science Direct. From the pharmacotherapeutic management point of view, any strategies for prevention should contain medical approaches. The bioactive compounds obtained from natural products have shown biological effects against different microorganisms for the treatment of these diseases. The main results showed antimicrobial, antiprotozoal, antifungal and antiviral effects such as HIV. Also, the molecular mechanisms, signalling pathways and action targets of natural compounds were highlighted, thus justifying bacterial and antifungal inhibition, apoptosis or reduction of viral replication. From the data of our study, we can conclude that natural compounds may be a significant source for adjuvant drugs / complementary therapies in the treatment of STDs. With all these benefits, the future must conduct extensive clinical trials and the development of pharmaceutical nanotechnologies for a greater therapeutic effect.

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The Caatinga endemic Manilkara rufula possesses remarkable activity against Trichomonas vaginalis and Tritrichomonas foetus

Cited by 9 publications

References 27 publications

Alpha-Pinene and Tannic Acid Inhibit Trichomonas vaginalis Protozoan Cells by Inducing Apoptosis

Alpha-Pinene and Tannic Acid Inhibit Trichomonas vaginalis Protozoan Cells by Inducing Apoptosis

Extraction, identification, and starch‐digestion inhibition of phenolics from Euryale ferox seed coat

Bioactive Compounds as Potential Agents for Sexually Transmitted Diseases Management: A Review to Explore Molecular Mechanisms of Action

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