The Campylobacter jejuni Type VI Secretion System Enhances the Oxidative Stress Response and Host Colonization

Liaw, Janie; Hong, Geunhye; Davies, Carolyn; Elmi, Abdi; Sima, F; Stratakos, Alexandros Ch.; Ştef, Lavinia; Peţ, Ioan; Hachani, Abderrahman; Corcionivoschi, Nicolae; Wren, Brendan W.; Gundogdu, Ozan; Dorrell, Nick

doi:10.3389/fmicb.2019.02864

Cited by 47 publications

(64 citation statements)

References 78 publications

(98 reference statements)

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“…This system is important for adaptation to bile acids and thus contributes to colonization of the intestine (Lertpiriyapong et al 2012). T6SS is a contactdependent secretion machinery capable of delivering effector proteins to both prokaryotic and eukaryotic cells (Liaw et al 2019). T6SS resembles an upside-down bacteriophage tail with homologous components.…”

Section: Secretion Systems-depending On the Functional Flagellar Appamentioning

confidence: 99%

Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Kreling

Falcone

Kehrenberg

et al. 2020

Appl Microbiol Biotechnol

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Infections caused by bacterial species from the genus Campylobacter are one of the four main causes of strong diarrheal enteritis worldwide. Campylobacteriosis, a typical food-borne disease, can range from mild symptoms to fatal illness. About 550 million people worldwide suffer from campylobacteriosis and lethality is about 33 million p.a. This review summarizes the state of the current knowledge on Campylobacter with focus on its specific virulence factors. Using this knowledge, multifactorial prevention strategies can be implemented to reduce the prevalence of Campylobacter in the food chain. In particular, antiadhesive strategies with specific adhesion inhibitors seem to be a promising concept for reducing Campylobacter bacterial load in poultry production. Antivirulence compounds against bacterial adhesion to and/or invasion into the host cells can open new fields for innovative antibacterial agents. Influencing chemotaxis, biofilm formation, quorum sensing, secretion systems, or toxins by specific inhibitors can help to reduce virulence of the bacterium. In addition, the unusual glycosylation of the bacterium, being a prerequisite for effective phase variation and adaption to different hosts, is yet an unexplored target for combating Campylobacter sp. Plant extracts are widely used remedies in developing countries to combat infections with Campylobacter. Therefore, the present review summarizes the use of natural products against the bacterium in an attempt to stimulate innovative research concepts on the manifold still open questions behind Campylobacter towards improved treatment and sanitation of animal vectors, treatment of infected patients, and new strategies for prevention. Key points • Campylobacter sp. is a main cause of strong enteritis worldwide. • Main virulence factors: cytolethal distending toxin, adhesion proteins, invasion machinery. • Strong need for development of antivirulence compounds.

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Section: Secretion Systems-depending On the Functional Flagellar Appamentioning

confidence: 99%

Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Kreling

Falcone

Kehrenberg

et al. 2020

Appl Microbiol Biotechnol

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“…To date, there is only one T6SS cluster described in Campylobacter [60], and it has been linked to a wide variety of phenotypes, including host cell adherence, cellular invasion and resistance to bile salt, and oxidative stress [67][68][69]. In addition,…”

Section: Distribution Of the T4ss And T6ss Gene Clustersmentioning

confidence: 99%

“…Campylobacter T6SS mutant strains exhibit colonization defects in both mouse [67] and chicken [69] in vivo infection models. The Campylobacter T6SS gene cluster is often encoded within the CjIE3 conjugative element in the chromosome of C. jejuni [61], but it can also be found in plasmids [70,71].…”

Section: Distribution Of the T4ss And T6ss Gene Clustersmentioning

confidence: 99%

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

Bravo

Katz

Porte

et al. 2020

Preprint

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ABSTRACTCampylobacter jejuni and Campylobacter coli are the leading causes of human gastroenteritis in the developed world and an emerging threat in developing countries. There is increasing evidence that the incidence of campylobacteriosis in Chile and other South American countries is greatly underestimated, mostly due to the lack of adequate diagnostic methods. Accordingly, there is limited genomic and epidemiological data from these countries, leaving an important knowledge gap in our understanding of the population structure, virulence potential, and antimicrobial resistance profiles of clinical Campylobacter strains. We recently published the whole-genome sequences of a collection of 68 C. jejuni and 12 C. coli strains isolated from patients with enteric infections during a 2-year period (2017-2019) attending Clinica Alemana in Santiago, Chile. In the present study, we sequenced an additional C. jejuni genome and performed a genome-wide analysis of the genetic diversity, virulence, and antimicrobial resistance potential of these 81 strains. cgMLST analysis identified a high genetic diversity of C. jejuni as well as 13 novel STs and alleles in both C. jejuni and C. coli. Pangenome and virulome analyses showed a differential distribution of virulence factors, including both plasmid and chromosomally encoded T6SSs and T4SSs. Resistome analysis predicted widespread resistance to fluoroquinolones, but a low rate of erythromycin resistance. This study provides valuable genomic and epidemiological data and highlights the need for larger genomic epidemiology studies to better understand the genetic diversity, virulence potential and antimicrobial resistance of human clinical Campylobacter strains in South America.DATA SUMMARYGenome sequences and assemblies of all C. jejuni and C. coli used in this study have been deposited in public databases (Table S1) and most strains have also been published as genome resource announcements [1,2].Bacterial draft genome sequence reannotations and nucleotide sequence fasta files used for virulome and plasmid screening analysis are available as a supplementary dataset on Zenodo (DOI: 10.5281/zenodo.3925206) https://doi.org/10.5281/zenodo.3925206.The reconstructed phylogenetic trees and associated metadata of clinical strains from Chile in the global context of C. jejuni and C. coli sequenced strains can be interactively visualized on Microreact at https://microreact.org/project/VbEQsZtQD.

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“…Flagella, which are modified by an O-linked glycosylation system, have been hypothesized not only to play a role in motility, but may also act as a secretion system discharging effectors such as CiaB. More recently, T6SSs have been identified in an increasing number of C. jejuni strains and the presence of T6SSs has been associated with a response to oxidative stress [5]. C. jejuni produces a cytolethal distending toxin which has been reported to arrest at the G 1 /S or G 2 /M transition of the cell cycle, depending on the cell type.…”

Section: Key Features and Discoveriesmentioning

confidence: 99%

“…80% of our authors rate our submission process as 'excellent' or 'very good'. 5. Your article will be published on an interactive journal platform with advanced metrics.…”

Section: Funding Informationmentioning

confidence: 99%

Microbe Profile: Campylobacter jejuni – survival instincts

Gundogdu

Wren

2020

Microbiology

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Top left) Campylobacter jejuni model. (Top right) C. jejuni transmission routes between humans, animals (particularly avian species) and the environment. (Bottom left) Are C. jejuni intracellular? Visualization of GFP C. jejuni within eukaryotic T84 cells using fluorescence confocal microscopy. Live bacteria (OD 600 of 0.1) were added and co-incubated with T84 cells for 2 h. Cells were fixed with paraformaldehyde, washed with PBS, stained and mounted. Cells were immunostained with antiwheat germ agglutinin to indicate membranes (red) with DAPI to indicate nuclei (blue), with C. jejuni GFP (green) (courtesy of Dr Abdi Elmi). (Bottom right) The major factors aiding C. jejuni survival include stress countering enzymes and their respective regulators, type VI secretion system (T6SS), O-and N-linked glycosylation systems, capsule, lipooligosaccharide, outer membrane vesicles and the flagella for motility and secretion of effectors.

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The Campylobacter jejuni Type VI Secretion System Enhances the Oxidative Stress Response and Host Colonization

Cited by 47 publications

References 78 publications

Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Campylobacter sp.: Pathogenicity factors and prevention methods—new molecular targets for innovative antivirulence drugs?

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

Microbe Profile: Campylobacter jejuni – survival instincts

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