The capability of non-native strains of <i>Bacteroides</i>
 bacteria to detect bacteriophages as faecal indicators in a tropical area

Sirikanchana, Kwanrawee; Wangkahad, Bencharong; Mongkolsuk, Skorn

doi:10.1111/jam.12646

Cited by 15 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detection of phages by strains GA17, GB124 show a certain geographic discontinuity, which was previously appreciated for strain HSP40 (Kator and Rhodes, 1992;McLaughlin and Rose, 2006;Sirikanchana et al, 2014). In Southern Europe, Tunissia and Colombia, most phage concentrations detected by GA17 ranged between 5x10 4 and 5x10…”

Section: B Thetaiotaomicron Spainmentioning

confidence: 61%

General and host-associated bacteriophage indicators of faecal pollution

Jebri¹,

Muniesa²,

Jofre³

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

View full text Add to dashboard Cite

The designations employed and the presentation of material throughout this publication do not imply the expression of any opinion whatsoever on the part of UNESCO concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The ideas and opinions expressed in this publication are those of the authors; they are not necessarily those of UNESCO and do not commit the Organization.

show abstract

Section: B Thetaiotaomicron Spainmentioning

confidence: 61%

General and host-associated bacteriophage indicators of faecal pollution

Jebri¹,

Muniesa²,

Jofre³

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

View full text Add to dashboard Cite

show abstract

“…The E. faecalis AIM06 and SR14 host strains demonstrated 100% specificity for human fecal origin (Wangkahad et al 2017), regardless of the diversity and morphology of phages recovered from the bacterial hosts in this study. Moreover, because B. fragilis phage ATCC 700786-B1 could be of either human or animal fecal origin (Sirikanchana et al 2014), the inability of E. faecalis host strains AIM06 and SR14 to host Bacteroides phages could reduce the probability of misinterpreting fecal sources.…”

Section: Discussionmentioning

confidence: 99%

“…The host range of phages isolated from enterococcal strains AIM06 and SR14 were tested in other enterococci as well as gram-negative and other gram-positive bacteria. The grampositive bacterial hosts used in this study comprised 25 previously isolated enterococcal host strains (Wangkahad et al 2017) (Sirikanchana et al 2014;Leknoi et al 2016). To evaluate the ability of phages to infect a range of bacterial hosts, we performed a spot test as previously described (Wangkahad et al 2015).…”

Section: Host Range Characterizationmentioning

confidence: 99%

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Booncharoen

Mongkolsuk

Sirikanchana

2018

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Enterococcus faecalis bacteria have been recently reported for their ability to host bacteriophages that are specifically from human sewage, suggesting their application to track human fecal contamination in water resources. However, little is known about the survivability of sewage-specific enterococcal bacteriophages in various water matrices under ambient and storage conditions. In this study, bacteriophages that were derived from the Thailand-isolated E. faecalis strains AIM06 and SR14 exhibited morphologies consistent with the Siphoviridae, Podoviridae, and Myoviridae families. Four representative bacteriophages were separately spiked into environmental water samples (n = 7) comprising freshwater and seawater with low- and high-pollution (LF, HF, LS, and HS, respectively) levels, defined according to Thailand Water Quality Standards. All bacteriophages decayed fastest in HS or HF samples at 30 °C, reaching a 5-log reduction in 2.2 to 9.8 days, and slowest in LS samples, requiring 8.8 to 23.5 days. The decay rates were 5 to 53 times lower at a storage temperature of 5 °C. HF samples could be stored for as little as 2.5 days to prevent the decay of 50% of the phages. Myoviridae phages decayed faster than Siphoviridae phages and Podoviridae phages in most water matrices at 30 °C. Moreover, the decay rates were 1.8 to 92 times slower in filtered samples, emphasizing a strong role for water constituents, i.e., suspended solids and natural microorganisms, in phage persistence. This study emphasized that differential enterococcal bacteriophage persistence should be considered when planning the monitoring and interpreting of fecal sources by microbial source tracking.

show abstract

“…Recent advancements in fecal indication incorporate microbial source tracking (MST) indicators. MST markers are also gastrointestinal microorganisms, but their presence in only specific hosts (e.g., humans, horses, pigs, and cattle) is beneficial for pollution source discrimination (Sirikanchana et al, 2014;Wangkahad et al, 2015;Ahmed et al, 2016;Garcia-Aljaro et al, 2018;Holcomb et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Human Fecal Pollution Monitoring and Microbial Risk Assessment for Water Reuse Potential in a Coastal Industrial–Residential Mixed-Use Watershed

et al. 2021

View full text Add to dashboard Cite

Rapid economic development has caused industrial expansion into residential communities, leading to higher fecal pollution loads that could be discharged into aquatic environments. However, little is known regarding the potential microbial impact on human health. This study investigated microbial contamination from coastal industrial–residential community areas in nine sampling sites in waterways during three dry events. A general microbial source tracking (MST) marker, GenBac3, was detected in all samples from all three events, indicating continuing fecal pollution in the area, mostly from human sewage contamination. This was shown by the human-specific genetic marker crAssphage (88.9%) and human polyomavirus (HPyVs; 92.6%) detection. Enteric human adenovirus (HAdV40/41) showed three positive results only from residential sites in the first event. No spatial difference was observed for MST markers and traditional fecal indicators (total coliforms and Escherichia coli) in each event. Still, a significantly lower abundance of GenBac3, HPyVs, and total coliforms in the first sampling event was detected. Spearman’s rho analysis indicated a strong correlation among certain pairs of microbial parameters. Multivariate analysis revealed two clusters of samples separated by land use type (industrial vs. residential). According to factor analysis of mixed data, the land use parameter was more associated with physicochemical parameters (i.e., salinity, conductivity, water temperature, and dissolved oxygen). A Quantitative Microbial Risk Assessment (QMRA) was then conducted to estimate the annual infection risks of HAdV40/41 for non-potable water reuse purposes using predicted concentrations from crAssphage and HPyVs. The highest risks (95th percentiles) were ranked by food crop irrigation, aquaculture, and toilet flushing, at 10–1, 10–2, and 10–3 per person per year (pppy). Required treatment levels to achieve a 10–4 pppy annual infection risk were estimated. QMRA-based water treatment scenarios were suggested, including chlorination for toilet flushing reuse and depth filtration prior to chlorination for aquaculture and food crop irrigation. Microbial monitoring combined with a QMRA could provide better insights into fecal pollution patterns and the associated risks, facilitating effective water quality management and appropriate prior treatments for water reuse.

show abstract

The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area

Cited by 15 publications

References 33 publications

General and host-associated bacteriophage indicators of faecal pollution

General and host-associated bacteriophage indicators of faecal pollution

Comparative persistence of human sewage-specific enterococcal bacteriophages in freshwater and seawater

Human Fecal Pollution Monitoring and Microbial Risk Assessment for Water Reuse Potential in a Coastal Industrial–Residential Mixed-Use Watershed

Contact Info

Product

Resources

About