The capsaicin test in mice for evaluating tachykinin antagonists in the spinal cord

Sakurada, Tsukasa; Katsumata, Kazushige; Tan-No, Koichi; Sakurada, Shinobu; Kisara, Kensuke

doi:10.1016/0028-3908(92)90057-v

Cited by 221 publications

(138 citation statements)

References 37 publications

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“…Capsaicin is used as a pharmacological tool for the study of nociception through direct activation of nociceptive fibers, especially type C (Sakurada et al, 1992). Capsaicin applied to skin, muscle, and other tissues has been shown to produce inflammation, to activate and to sensitize trigeminal and spinal small-diameter nociceptive afferents, as well as dorsal horn neurons.…”

Section: Discussionmentioning

confidence: 99%

“…The activation of nociceptors by capsaicin occurs through its binding to vanilloid receptors (TRPV1), which induces the influx of cations producing depolarization and excitation of neurons that cause the release of neuropeptides such as tachykinins, substance P, calcitonin gene-related peptide, excitatory amino acids (aspartate and glutamate), nitric oxide, and proinflammatory mediators from the peripheral terminals (Sakurada et al, 1992). In this context, the antinociceptive effect observed for p-cymene in the capsaicin test may be a result of its possible inhibition of neurotransmitters involved in activation of sensory fibers responsible for transmission of nociceptive stimuli.…”

Section: Discussionmentioning

confidence: 99%

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p-Cymene reduces orofacial nociceptive response in mice

Santana¹,

Quintans‐Júnior²,

Cavalcanti³

et al. 2011

Rev. bras. farmacogn.

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This study investigated the possible antinociceptive effect of p-cymene in different tests of orofacial nociception. The animals (mice) were pretreated (i.p.) with p-cymene (25, 50, 100 mg/kg), morphine (5 mg/kg), or vehicle (0.2% Tween 80+saline), and were then subsequently administered, subcutaneously into their upper lip: formalin, capsaicin, and glutamate. The nociceptive behavior response was characterized by the time in s that the mice remained rubbing the orofacial region, for a period of 40 min in the formalin test (first phase, 0-6 min; and second phase, 21-40 min), and for 42 and 15 min in the capsaicin and glutamate tests, respectively. To verify the possible opioid involvement in the antinociceptive effects, naloxone (i.p.) was administered into the mice 15 min prior to the pretreatment with p-cymene (100 mg/kg). Finally, whether or not the p-cymene evoked any change in motor performance in the Rota-rod test was evaluated. The results showed that the treatment with p-cymene, at all doses, reduced (p<0.001) the nociceptive behavior in all nociception tests. The antinociceptive effect of p-cymene was antagonized by naloxone (1.5 mg/kg). Additionally, mice treated with p-cymene did not show any change in motor performance. In conclusion, p-cymene attenuated orofacial nociception, suggesting an involvement of the opioid system in this effect. Thus, p-cymene might represent an important biomolecule for management and/or treatment of orofacial pain.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

p-Cymene reduces orofacial nociceptive response in mice

Santana¹,

Quintans‐Júnior²,

Cavalcanti³

et al. 2011

Rev. bras. farmacogn.

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“…The procedure used was similar to that described previously (Sakurada et al, 1992). The animals (n = 6-8) were placed individually in transparent glass cylinders.…”

Section: Capsaicin-induced Painmentioning

confidence: 99%

Antinociceptive effects of Abarema cochliacarpos (B.A. Gomes) Barneby & J.W.Grimes (Mimosaceae)

Silva¹,

Esquibel²,

Alves³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: "Efeito antinociceptivo de Abarema cochliacarpos (B.A. Gomes) Barneby & J.w.Grimes (Mimosaceae)". No presente trabalho foram avaliados a atividade antinociceptiva e o perfil fitoquímico dos extratos aquosos e metanólico produzidos com a casca do caule de Abarema cochliacarpos, uma espécie de Mata Atlântica com diversas indicações populares. Todos os extratos apresentaram atividade analgésica quando avaliados pelo teste das contorções abdominais induzidas pelo ácido acético via intraperitonial, apresentando respostas superiores às drogas usadas como referência, bem como no modelo da dor induzida por capsaicina. A avaliação fitoquímica demonstrou a presença de saponinas, catequinas, taninos, fenóis e antraquinonas.Unitermos: Abarema cochliacarpos, Mimosaceae, atividade analgésica, plantas medicinais, perfil fitoquímico. ABSTRACT:In this study, we investigated the analgesic activity of crude aqueous and methanol extracts obtained from Abarema cochliacarpos bark in mice, and analyzed its phytochemical profile. All the extracts exhibited analgesic properties against the writhing test in mice, but the aqueous and methanol extracts were more active, and more potent than two known analgesic and anti-inflammatory drugs used as reference. They were also active against the capsaicin-model, but inactive when evaluated in the hot-plate test. Phytochemical studies revealed the presence of saponins, catechins, tannins, phenols and anthraquinones.

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“…Intraplantar injection of capsaicin in the mouse hindpaw induces a single phase nociceptive response similar to that displayed in the former phase of the formalin test (Sakurada et al, 1992). The response to capsaicin is attributed to direct activation of speci®c receptors leading to stimulation of Ad and C a erent nociceptive ®bres, and to the release of glutamate and neuropeptides (Dray, 1995).…”

Section: Discussionmentioning

confidence: 81%

“…A similar procedure was used to assess the TKI e ect on the capsaicin-induced neurogenic pain (Sakurada et al, 1992). Following 15 min adaptation to the observation chamber, mice were treated with either the vehicle, TKI (13.6 ± 136 mmol kg 71 , i.p.)…”

Section: Formalin Testmentioning

confidence: 99%

Evidence for activation of the tissue kallikrein‐kinin system in nociceptive transmission and inflammatory responses of mice using a specific enzyme inhibitor

Emim¹,

Souccar²,

Castro³

et al. 2000

British J Pharmacology

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1 The pharmacological activity of phenylacetyl-Phe-Ser-Arg-N-(2,4-dinitrophenyl)-ethylenediamine (TKI), a tissue kallikrein speci®c inhibitor, was assessed using models of nociception and in¯ammation in mice. 2 Injection of TKI (13.6 ± 136 mmol kg 71 , i.p. or 41 ± 410 mmol kg 71 , s.c.) produced a dose-related inhibition of the acetic acid-induced writhes (by 37 to 85% or 34 to 80%, respectively). The antinociceptive activity of TKI (41 mmol kg 71 , i.p.) was maximal after 30 min injection and lasted for 120 min. The e ect was unaltered by pretreatment with naloxone (8.2 mmol kg 71 , s.c.) or bilateral adrenalectomy. 3 TKI (41 and 136 mmol kg 71 , i.p.) produced a dose-related decrease of the late phase of formalininduced nociception by 79 and 98%, respectively. At 136 mmol kg 71 , i.p., TKI also shortened the duration of paw licking in the early phase by 69%. TKI (41 and 136 mmol kg 71 , i.p.) also reduced the capsaicin-induced nociceptive response (by 51 to 79%). 4 TKI (41 mmol kg 71 , i.p. or 410 mmol kg 71 , s.c.) reduced the oedematogenic response, from the second to the ®fth hour after carrageenin injection by 36 to 30% or by 47 to 39%, respectively. 5 Pretreatment with TKI (41 mmol kg 71 , i.p.) reduced the capsaicin-induced neurogenic in¯ammation in the mouse ear by 54%. 6 It is concluded that TKI presents antinociceptive and antiin¯ammatory activities mediated by inhibition of kinin formation by tissue kallikrein in mice. The results also indicate that the tissue kallikrein-dependent pathway contributes to kinin generation in nociceptive and in¯ammatory processes in mice.

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The capsaicin test in mice for evaluating tachykinin antagonists in the spinal cord

Cited by 221 publications

References 37 publications

p-Cymene reduces orofacial nociceptive response in mice

p-Cymene reduces orofacial nociceptive response in mice

Antinociceptive effects of Abarema cochliacarpos (B.A. Gomes) Barneby & J.W.Grimes (Mimosaceae)

Evidence for activation of the tissue kallikrein‐kinin system in nociceptive transmission and inflammatory responses of mice using a specific enzyme inhibitor

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