The carbon and hydrogen stable isotope composition of bacteriogenic methane: A laboratory study using a landfill inoculum

Waldron, Susan; Watson-Craik, Irene A.; Hall, Anthony; Fallick, Anthony E.

doi:10.1080/01490459809378073

Cited by 37 publications

(35 citation statements)

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“…They attributed the high concentration of H 2 (80 %) used in their laboratory incubations as a cause for very negative values of δ 2 H-CH 4 in the cultures (Burke, 1993;Chidthaisong., et al, 2002;Sugimoto and Wada, 1995). The outcome of the current experiments (Tables 4 and 5) supports the concept that CO 2 reduction may result in CH 4 with negative δ 2 H value comparable to that found in acetate derived CH 4 (Chidthaisong et al, 2002;Sugimoto and Wada, 1995;Waldron et al, 1998). In Experiment 1, while the carbon fractionation factors indicate distinct pathways, the corresponding δ 2 H-CH 4 values are quite similar, suggesting that δ 2 H-CH 4 is largely independent of the methanogenic pathway.…”

Section: δ 2 H For Determination Of Methanogenesis Pathwaysupporting

confidence: 73%

“…Small isotopic fractionation factor α values (<1.055) are typical of the prevalent operation of acetoclastic methanogenesis, whereas higher α values (<1.065) are indicative of hydrogenotrophic methanogenesis (Whiticar et al, 1986). While these general ranges appear to be correct in most cases, the strict interpretation of CH 4 carbon isotopic data is not warranted (Chanton et al, 2004;Chidthaisong et al, 2002;Waldron et al, 1998;Whiticar, 1999). For example, high α values are typical of hydrogenotrophic methanogenesis, but in an environment with a high supply of free energy available to the methanogens, hydrogenotrophic methanogenesis may also cause a low α value (Conrad, 2005;Penning et al, 2005).…”

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confidence: 96%

“…The apparent carbon isotopic fractionation factor (α c ) and isotopic effect (ε c ) were calculated from the ratio of δ 13 C-CO 2 to δ 13 C-CH 4 according to Equations 3 and 4, which are α c = (δ 13 C-CO 2 + 10 3 )/ (δ 13 C-CH 4 + 10 3 ) and ε c = (δ 13 C-CO 2 -δ 13 C-CH 4 ). cleaved to form CH 4 and CO 2 , if CH 4 production is dominated by acetoclastic methanogenesis, the δ 13 C-CH 4 and δ 13 C-CO 2 will generally approximate the δ 13 C of the acetate-methyl group and the δ 13 C of the acetate-carboxyl group, respectively (Waldron et al, 1998). As such, the carbon isotopic composition of acetate (δ 13 C-acetate) and ɛ ma can be estimated by mass balance using the data of δ 13 C-CH 4 and δ 13 C-CO 2 .…”

Section: Carbon Isotopesmentioning

confidence: 99%

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Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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Carbon and hydrogen isotope compositions of CH 4 generated via methanogenesis in cultures of South Sumatra Basin (SSB) coalbed methane (CBM) formation waters grown on coal, acetate and H 2 +CO 2 were investigated. CH 4 production and molecular analysis confirmed the presence of active microbial communities that are able to convert coal into CH 4 using both acetoclastic and hydrogenotrophic pathways. The representative bacterial sequences were dominated by Bacteroidetes, Firmicutes and Deltaproteobacteria, while Methanosaeta and Methanosarcina were the most prevalent archaeal methanogens present in the cultures.CH 4 produced in this study's culturing experiments has δ 13 C values in the range of -50 ‰ to -20 ‰, with most values falling outside the current understanding of the carbon isotopic boundaries for biogenic CH 4 (-110 ‰ to -30 ‰). However, the corresponding apparent carbon isotopic α factor (α c = 1.02±0.006), and isotopic effect (ε c = -20.1 ‰ ±15.3) showed that CH 4 in SSB cultures was predominantly produced by acetoclastic methanogenesis, which is consistent with the results of molecular DNA analysis. In addition, the calculated contribution of CO 2 reduction from the δ 13 C values of coaltreated cultures was overall < 50 %, further confirming the high contribution of the acetoclastic pathway to CH 4 production in the SSB cultures. The outcome of this experimental study also suggests that δ 2 H-CH 4 values may not provide a reliable basis for distinguishing methanogenic pathways, while apparent carbon isotopic fractionation factor (α c ) and isotope effect (ε c ) are considered more useful indicators of the methanogenic pathway.The high δ 13 C-CH 4 values (>-30 ‰) and the dominance of Methanosaeta over Methanosarcina indicate that methanogens within the SSB cultures were operating at low substrate concentrations. An unusually positive δ 13 C-CH 4 suggests a substrate depletion effect, which is thought to be related to a decrease in the relative abundance of key bacterial coal degraders with formation water inoculum storage time. Closer observation of δ 13 C-CH 4 values during the growth of cultures within a single experiment also showed a 13 C-enrichment trend over time. At log phase of growth, the CH 4 produced was 13 C-depleted when compared to the stationary phase that also indicates substrate depletion effects. Finally, the δ 13 C-CH 4 values encountered in this study (as high as -20 ‰) highlight the possible positive extension of δ 13 C-CH 4 values of acetoclastic methanogenesis from those currently reported in the literature for natural and experimental samples (as high as -30 ‰).Keywords: Biogenic gas, coal, culture experiment, isotopic composition INTRODUCTIONArchaeal methanogens are the only known microorganisms capable of forming CH 4 . These strictly anaerobic microbes are restricted to utilizing simple compounds that contain no more than two carbon atoms. As such, complex organic compounds in anoxic environments (e.g., marine sediments, rice paddies, subsurface shales and coalbeds) ...

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Section: δ 2 H For Determination Of Methanogenesis Pathwaysupporting

confidence: 73%

mentioning

confidence: 96%

Section: Carbon Isotopesmentioning

confidence: 99%

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Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Susilawati

Golding

Baublys

et al. 2016

International Journal of Coal Geology

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“…The acetoclastic pathway is thought to produce δD-CH4 in the range of -250‰ to -400‰ whereas CO2 reduction typically produces δD-CH4 between -170‰ to -250‰. However, the above generalization may not always be correct and the boundary should not be strictly applied Waldron et al, 1998;. In such conditions, e.g.…”

Section: Coalbed Gas Generationmentioning

confidence: 99%

“…Similarly, naturally produced CH4 from microbial CO2 reduction can also have δ 13 C-CH4 that resemble thermogenic origin (> ̶ 50‰) but with δ 13 C-CO2 of +22 (Martini et al, 1998;Martini et al, 2008). There are also conditions where δD-CH4 from the CO2 reduction pathway is indistinguishable from the acetoclastic pathway (Sugimoto and Wada, 1993;Waldron et al, 1998). For better interpretation of both origin and pathways generation, it has been suggested to use multi-element isotope approaches .…”

Section: Coalbed Gas Generationmentioning

confidence: 99%

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

Susilawati¹

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Biogenic coal bed methane (CBM) has gained considerable interest because of its potential to be regenerated. The research reported in this thesis evaluated the potential to develop Indonesian coals as CH 4 bioreactors using culture enrichment, molecular phylogenetic and isotopic composition analysis methods. The results increase the current understanding on microbial methanogenesis in coal, with a specific focus on Indonesian coals.Indonesian coals range in rank from lignite to bituminous, with anthracites occurring in specific areas. The coals are low in ash yield and dominated by vitrinite group macerals, with abundant liptinite group macerals, which is thought to make them good targets for methanogenesis.Stable isotope analysis of gas from pilot production wells confirmed the hypothesis that Indonesian CBM is partly biogenic in origin. Culture enrichment studies of formation water from these CBM pilot wells also indicated the presence of active coal to CH 4 consortia that has the capability for degrading native South Sumatra Basin (SSB) coals and foreign Surat Basin coal from Australia. Among the Indonesian coal basins, the SSB currently has the greatest CBM potential and good accessibility for gas, water, and coal sampling that is required to assess the biogenic CH 4 potential of Indonesian coal. Therefore, SSB was targeted for the experiments conducted in this thesis. The investigation of the microbial methanogen community structure grown on different coal substrates showed temporal changes in community structure over time, and suggested some influence of coal substrate on the microbial community composition. The community structure exhibited greater similarity when grown on coal from the same seam but of different ranks (Mangus iii sub-bituminous (SB) Rv 0.5% and Mangus Anthracite (A) Rv 2.2%) than for coal of similar types (Mangus SB Rv 0.5% and Burung SB Rv 0.39%). The obligate acetoclastic Methanosaeta members favor Burung SB coal, while metabolically versatile Methanosarcina members favor the Mangus coals and the obligate hydrogenotrophic methanogens are significant in the control cultures without coal. Regardless of the community structure similarities across all coal cultures, more CH 4 was generated from the lower rank sub-bituminous coal cultures relative to the one high-rank semi anthracite coal. These results suggest a potential relationship between coal type and rank, microbial community composition and CH 4 production, which warrants further investigation.While CH 4 measurements and molecular phylogenetic analysis confirmed the production of biogenic CH 4 in the cultures, the gas sampled from the culture headspace had δ 13 C-CH 4 values ( 52.2‰ to 22.6‰) that mostly fell outside the range currently considered to indicate a biogenic origin. In this study, the apparent carbon fractionation factor (α c =1.02±0.006) and isotope effect (ε c = 20.1‰±15.3) were found to be more useful indicators of methanogenic pathways than the absolute δ 13 C-CH 4 values. Both values agreed with the calculat...

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References Part I

Meier‐Augenstein¹

2010

Stable Isotope Forensics

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The carbon and hydrogen stable isotope composition of bacteriogenic methane: A laboratory study using a landfill inoculum

Abstract: 1998) The carbon and hydrogen stable isotope composition of bacteriogenic methane: A laboratory study using a landfill inoculum, Geomicrobiology Journal, 15:3, 157-169,

Cited by 37 publications

References 27 publications

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Carbon and hydrogen isotope fractionation during methanogenesis: A laboratory study using coal and formation water

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

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