The CC and CXC chemokine receptors in turbot (Scophthalmus maximus L.) and their response to Aeromonas salmonicida infection

Zhao, Shoucong; Li, Yuqing; Cao, Min; Hu, Jie; Xue, Ting; Li, Chao; Fu, Qiang

doi:10.1016/j.dci.2021.104155

Cited by 10 publications

(2 citation statements)

References 69 publications

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“…We observed that brain cxcr3 was significantly up-regulated by A. salmonicida infection ( Figure 4 ). Consistently, transcriptional profiles of cxcr3 subtypes were significantly altered by a bacterial and viral infection, LPS or polyI:C stimulation in teleost species, including rainbow trout, turbot, largemouth bass ( Micropterus salmoides ), and black rockfish [ 57 , 68 , 78 , 82 ]. In rainbow trout, cxcr3 subtypes were differently induced by inflammatory stimulants and cytokines in head kidney cells and macrophages [ 59 , 76 , 83 ].…”

Section: Discussionmentioning

confidence: 82%

“…In this study, brain cxcr1.1 and cxcr1.2 showed different transcriptional regulation in response to bacterial infections ( Figure 3 ). A recent study showed cxcrs exhibited tissue-specific and time-dependent regulation of transcription in the head kidney, liver, and gill after A. salmonicida infection in turbot ( Scophthalmus maximus ) and black rockfish ( Sebastes schlegelii ) [ 68 , 78 ]. These results suggest that cxcr1 subtypes might be differently involved in the response to disease in both brain and peripheral tissues.…”

Section: Discussionmentioning

confidence: 99%

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Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis

Hou,

Zhao,

Perdiguero

et al. 2024

Biomolecules

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Chemokines are cytokines with chemoattractant capacities that exert their physiological functions through the binding of chemokine receptors. Thus, chemokine and receptor complexes exert important roles in regulating development and homeostasis during routine immune surveillance and inflammation. Compared to mammals, the physiology and structure of chemokine receptors in fish have not been systematically studied. Furthermore, the salmonid-specific whole genome duplication has significantly increased the number of functional paralogs of chemokine receptors. In this context, in the current study, trout exhibited 17 cxcr genes, including 12 newly identified and 5 previously identified receptors. Interestingly, gene expression of brain cxcr1 and cxcr4, kidney cxcr3 and cxcr4, and spleen cxcr3, cxcr4, and cxcr5 subtypes were altered by bacterial infection, whereas brain cxcr1, kidney cxcr1 and cxcr7, and liver cxcr2, cxcr3, and cxcr4 subtypes were changed in response to environmental changes. Based on protein structures predicted by ColabFold, the conserved amino acids in binding pockets between trout CXCR4.1 subtypes and human CXCR4 were also analyzed. Our study is valuable from a comparative point of view, providing new insights into the identification and physiology of salmonid chemokine receptors.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis

Hou,

Zhao,

Perdiguero

et al. 2024

Biomolecules

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Comparative Transcriptome Analysis of Spleen Reveals Potential Regulation of Genes and Immune Pathways Following Administration of Aeromonas salmonicida subsp. masoucida Vaccine in Atlantic Salmon (Salmo salar)

Zhang

et al. 2022

Mar Biotechnol

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Aeromonas salmonicida is a global fish pathogen. Aeromonas salmonicida subsp. masoucida (ASM) is classified as atypical A. salmonicida and caused huge losses to salmonid industry in China. Hence, it is of great significance to develop ASM vaccine and explore its protection mechanism in salmonids. In this regard, we conducted RNA-seq analysis with spleen tissue of Atlantic salmon after ASM vaccination to reveal genes, their expression patterns, and pathways involved in immune protections. In our results, a total of 441.63 million clean reads were obtained, and 389.37 million reads were mapped onto the Atlantic salmon reference genome. In addition, 1125, 2126, 1098, 820, and 1351 genes were significantly up-regulated, and 747, 2626, 818, 254, and 908 genes were significantly down-regulated post-ASM vaccination at 12 h, 24 h, 1 month, 2 months, and 3 months, respectively. Subsequent pathway analysis revealed that many differentially expressed genes (DEGs) following ASM vaccination were involved in cytokine-cytokine receptor interaction ( TNFRSF11b , IL-17RA , CCR9 , and CXCL11 ), HTLV-I infection ( MR1 and HTLV-1 ), MAPK signaling pathway ( MAPK , IL8 , and TNF-α-1 ), PI3K-Akt signaling pathway ( PIK3R3 , THBS4 , and COL2A1 ), and TNF signaling pathway ( PTGS2 , TNFRSF21-l , and CXCL10 ). Finally, the results of qRT-PCR showed a significant correlation with RNA-seq results, suggesting the reliability of RNA-seq for gene expression analysis. This study provided insights into regulation of gene expression and their involved pathways in Atlantic salmon spleen in responses to vaccine, and set the foundation for further study on the vaccine protective mechanism in Atlantic salmon as well as other teleost species. Supplementary Information The online version contains supplementary material available at 10.1007/s10126-021-10089-6.

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CC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolutionary analysis, and expression patterns after Aeromonas Salmonicida infection

Zhao

et al. 2022

Aquaculture

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The CC and CXC chemokine receptors in turbot (Scophthalmus maximus L.) and their response to Aeromonas salmonicida infection

Cited by 10 publications

References 69 publications

Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis

Pleiotropic Role of Rainbow Trout CXCRs in Response to Disease and Environment: Insights from Transcriptional Signatures and Structure Analysis

Comparative Transcriptome Analysis of Spleen Reveals Potential Regulation of Genes and Immune Pathways Following Administration of Aeromonas salmonicida subsp. masoucida Vaccine in Atlantic Salmon (Salmo salar)

CC chemokines and their receptors in black rockfish (Sebastes schlegelii): Characterization, evolutionary analysis, and expression patterns after Aeromonas Salmonicida infection

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