The CCAAT element in the <i>CIWI</i> promoter regulates transcriptional initiation in chicken primordial germ cells

Sohn, Yoon Ah; Lee, Sang In; Kim, Hyun Jeong; Kim, Ki‐Hyun; Park, Tae Sub; Han, Jae Yong

doi:10.1002/mrd.22356

Cited by 4 publications

(4 citation statements)

References 40 publications

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“…The C 2 H 2 Zn-finger domains of Mamo short isoform are homologous to those of human Sp1; 20 Sp1-related transcription factors regulate gene expression in germ cells in vertebrates 21–23 . For example, chicken Sp1 promotes vas expression in PGCs 24 . Moreover, Ovo has conserved roles in germline development in mouse and Drosophila 16,33 .…”

Section: Discussionmentioning

confidence: 99%

“…C 2 H 2 Zn-finger domains are among the most common domains in the transcription factors of metazoans 19 . The Zn-finger domains of Mamo are homologous to those of human Sp1; 20 in vertebrates, Sp1-related transcription factors regulate gene expression in germ cells 21–24 . Accordingly, we focused on Mamo and investigated its role in vas activation in Drosophila embryos.…”

Section: Introductionmentioning

confidence: 99%

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A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos

et al. 2019

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Expression of the vasa gene is associated with germline establishment. Therefore, identification of vasa activator(s) should provide insights into germline development. However, the genes sufficient for vasa activation remain unknown. Previously, we showed that the BTB/POZ-Zn-finger protein Mamo is necessary for vasa expression in Drosophila. Here, we show that the truncated Mamo lacking the BTB/POZ domain (MamoAF) is a potent vasa activator. Overexpression of MamoAF was sufficient to induce vasa expression in both primordial germ cells and brain. Indeed, Mamo mRNA encoding a truncated Mamo isoform, which is similar to MamoAF, was predominantly expressed in primordial germ cells. The results of our genetic and biochemical studies showed that MamoAF, together with CBP, epigenetically activates vasa expression. Furthermore, MamoAF and the germline transcriptional activator OvoB exhibited synergy in activating vasa transcription. We propose that a Mamo-mediated network of epigenetic and transcriptional regulators activates vasa expression.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos

et al. 2019

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“…In C. elegans , mutations in nfya-1 affect the development of germ cells and also reduce the number of sperm [55]. Moreover, NF-Y is greatly affected by the CCAAT motif in terms of its transcriptional activity regarding Miwi and CIWI gene [56, 57]. Additionally, SP3 is one of the Sp family of TFs, which is characterized by three conserved zinc fingers [58], and positively or negatively controls the transcriptional activity of numerous genes through binding to the GC box in cis -regulatory elements [59].…”

Section: Discussionmentioning

confidence: 99%

Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells

Jin

Lee

Hwang

et al. 2017

J Animal Sci Biotechnol

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BackgroundPrimordial germ cells (PGCs), the precursors of functional gametes, have distinct characteristics and exhibit several unique molecular mechanisms to maintain pluripotency and germness in comparison to somatic cells. They express germ cell-specific RNA binding proteins (RBPs) by modulating tissue-specific cis- and trans-regulatory elements. Studies on gene structures of chicken vasa homologue (CVH), a chicken RNA binding protein, involved in temporal and spatial regulation are thus important not only for understanding the molecular mechanisms that regulate germ cell fate, but also for practical applications of primordial germ cells. However, very limited studies are available on regulatory elements that control germ cell-specific expression in chicken. Therefore, we investigated the intricate regulatory mechanism(s) that governs transcriptional control of CVH.ResultsWe constructed green fluorescence protein (GFP) or luciferase reporter vectors containing the various 5′ flanking regions of CVH gene. From the 5′ deletion and fragmented assays in chicken PGCs, we have identified a CVH promoter that locates at −316 to +275 base pair fragment with the highest luciferase activity. Additionally, we confirmed for the first time that the 5′ untranslated region (UTR) containing intron 1 is required for promoter activity of the CVH gene in chicken PGCs. Furthermore, using a transcription factor binding prediction, transcriptome analysis and siRNA-mediated knockdown, we have identified that a set of transcription factors play a role in the PGC-specific CVH gene expression.ConclusionsThese results demonstrate that cis-elements and transcription factors localizing in the 5′ flanking region including the 5′ UTR and an intron are important for transcriptional regulation of the CVH gene in chicken PGCs. Finally, this information will contribute to research studies in areas of reproductive biology, constructing of germ cell-specific synthetic promoter for tracing primordial germ cells as well as understanding the transcriptional regulation for maintaining germness in PGCs.

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“…and Sohn et al . reported that DNA methylation and NF-Y co-regulate Piwil1 expression in chickens [20, 21]. Hou et al .…”

Section: Discussionmentioning

confidence: 99%

DNA methylation-mediated transcription factors regulate <i>Piwil1</i> expression during chicken spermatogenesis

Qiu

Chang

et al. 2016

Journal of Reproduction and Development

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The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 promoter in five different spermatogenic cell lines using direct bisulfite pyrosequencing and determined that methylation correlates negatively with germ cell type-specific expression patterns of piwil1. We demonstrated that methylation of the −148 CpG site, which is the predicted binding site for the transcription factors TCF3 and NRF1, was differentially methylated in different spermatogenic cells. This site was completely methylated in PGCs (primordial germ cells), but was unmethylated in round spermatids. A similar result was obtained in the region from +121 to +139 CpG sites of the Piwil1 promoter CpG island, which was predicted to contain SOX2 binding sites. In addition, demethylation assays further demonstrated that DNA methylation indeed regulates Piwil1 expression during chicken spermatogenesis. Combined with transcription factor binding site prediction, we speculate that methylation influences the recruitment of corresponding transcription factors. Collectively, we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation.

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The CCAAT element in the CIWI promoter regulates transcriptional initiation in chicken primordial germ cells

Cited by 4 publications

References 40 publications

A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos

A truncated form of a transcription factor Mamo activates vasa in Drosophila embryos

Regulatory elements and transcriptional control of chicken vasa homologue (CVH) promoter in chicken primordial germ cells

DNA methylation-mediated transcription factors regulate <i>Piwil1</i> expression during chicken spermatogenesis

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