The CCR4‐NOT complex component NOT1 regulates RNA‐directed DNA methylation and transcriptional silencing by facilitating Pol IV‐dependent siRNA production

Zhou, Hao-Ran; Lin, Rong-Nan; Huang, Huanwei; Li, Lin; Cai, Tao; Zhu, Jian‐Kang; Chen, She; He, Xin‐Jian

doi:10.1111/tpj.14818

Cited by 11 publications

(8 citation statements)

References 76 publications

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“…Additional transcription factor genes, such as homologues to Arabidopsis WRI2, LEC2, EFM, NAC56, are also upregulated in all three reproductive tissue types, but their functions in moss are unknown and not easy to extrapolate from their functions in Arabidopsis. A homologue to CCR4-NOT complex component NOT1, which in Arabidopsis regulates RNA-directed methylation and transcriptional silencing (Zhou et al, 2020), is also elevated in the reproductive organs and the green sporophytes. AtNOT1 is necessary for example proper male germ cell development, pollen germination and embryogenesis (Motomura et al, 2020;Pereira et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

MS1/MMD1 homologues in the moss Physcomitrium patens are required for male and female gametogenesis

et al. 2022

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Summary The Arabidopsis Plant HomeoDomain (PHD) proteins AtMS1 and AtMMD1 provide chromatin‐mediated transcriptional regulation essential for tapetum‐dependent pollen formation. This pollen‐based male gametogenesis is a derived trait of seed plants. Male gametogenesis in the common ancestors of land plants is instead likely to have been reminiscent of that in extant bryophytes where flagellated sperms are produced by an elaborate gametophyte generation. Still, also bryophytes possess MS1/MMD1‐related PHD proteins. We addressed the function of two MS1/MMD1‐homologues in the bryophyte model moss Physcomitrium patens by the generation and analysis of reporter and loss‐of‐function lines. The two genes are together essential for both male and female fertility by providing functions in the gamete‐producing inner cells of antheridia and archegonia. They are furthermore expressed in the diploid sporophyte generation suggesting a function during sporogenesis, a process proposed related by descent to pollen formation in angiosperms. We propose that the moss MS1/MMD1‐related regulatory network required for completion of male and female gametogenesis, and possibly for sporogenesis, represent a heritage from ancestral land plants.

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Section: Discussionmentioning

confidence: 99%

MS1/MMD1 homologues in the moss Physcomitrium patens are required for male and female gametogenesis

et al. 2022

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“…Our work mainly focused on the cytoplasmic role of RNA deadenylases and directly demonstrated the poly(A) tail regulation of transposons using ONT-DRS. A previous report suggested a nuclear role of the CCR4-NOT complex in Arabidopsis as one of the essential elements for RdDM 45 . This is reminiscent of what is known in Drosophila that CCR4 co-transcriptionally represses transposon in association with Piwi 30 .…”

Section: Discussionmentioning

confidence: 95%

Poly(A) tail length regulation by mRNA deadenylases is critical for suppression of transposable elements

Lei

Yan

et al. 2023

Preprint

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Transposons are mobile genetic elements that can impair the host genome stability and integrity. In plants, suppression of transposons is thought to be mediated mainly by small RNAs; however, the role of RNA decay in posttranscriptional repression of transposons is unknown. Here we show that RNA deadenylation is critical for controlling transposons in Arabidopsis. Previously, we demonstrated that transposon RNAs often harbor structural aberrancy owing to its inherently suboptimal codon usage and ribosome stalling. Such RNA aberrancy is monitored and resolved by RNA decay which is initiated by removal of poly(A) tail or deadenylation. The CCR4-NOT complex is a primary RNA deadenylase in Arabidopsis, and we found that it is required for stable repression of transposons. Intriguingly, RNA deadenylation controls transposons that are not targeted by cytoplasmic secondary small RNAs, which implies a target-specific regulation of transposon by the host. Our study suggests a previously unknown mechanism for transposon repression mediated by RNA deadenylation and unveils a complex nature of the host strategy to maintain the genome integrity.

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“…Additional transcription factor genes, such as homologs to Arabidopsis WRI2 , LEC2, EFM, NAC56 , are also up-regulated in all three reproductive tissue types, but their functions in moss are unknown and not easy to extrapolate from their functions in Arabidopsis. A homolog to CCR4-NOT complex component NOT1, which in Arabidopsis regulates RNA-directed methylation and transcriptional silencing (Zhou et al ., 2020), is also elevated in the reproductive organs and the green sporophytes. AtNOT1 is necessary for e.g.…”

Section: Discussionmentioning

confidence: 99%

MS1/MMD1homologs in the mossP. patensare required for male and female gametogenesis and likely for sporogenesis

Landberg

López-Obando

Sánchez-Vera

et al. 2022

Preprint

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The Arabidopsis Plant HomeoDomain (PHD) proteins AtMS1 and AtMMD1 provide chromatin-mediated transcriptional regulation essential for tapetum-dependent pollen formation. Such pollen-based male gametogenesis is a derived trait of seed plants. Male gametogenesis in the common ancestors of land plants is instead likely to have been reminiscent of that in extant bryophytes where flagellated sperms are produced by an elaborate gametophyte generation. Still, also bryophytes possess MS1/MMD1-related PHD proteins. We addressed the function of two MS1/MMD1-homologs in the bryophyte model moss Physcomitrium patens by the generation and analysis of reporter and loss-of-function lines. The two genes are together essential for both male and female fertility by providing cell autonomous functions in the gamete-producing inner cells of antheridia and archegonia. They are furthermore expressed in the diploid sporophyte generation suggesting a function during sporogenesis, a process proposed related by descent to pollen formation in angiosperms. We propose that the moss MS1/MMD1-related regulatory network required for completion of male and female gametogenesis and possibly for sporogenesis, represent a heritage from ancestral land plants.

show abstract

The CCR4‐NOT complex component NOT1 regulates RNA‐directed DNA methylation and transcriptional silencing by facilitating Pol IV‐dependent siRNA production

Cited by 11 publications

References 76 publications

MS1/MMD1 homologues in the moss Physcomitrium patens are required for male and female gametogenesis

MS1/MMD1 homologues in the moss Physcomitrium patens are required for male and female gametogenesis

Poly(A) tail length regulation by mRNA deadenylases is critical for suppression of transposable elements

MS1/MMD1homologs in the mossP. patensare required for male and female gametogenesis and likely for sporogenesis

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