2015
DOI: 10.1074/jbc.m114.616490
|View full text |Cite
|
Sign up to set email alerts
|

The Cdc20-binding Phe Box of the Spindle Checkpoint Protein BubR1 Maintains the Mitotic Checkpoint Complex During Mitosis

Abstract: Background:The spindle checkpoint protein BubR1 inhibits the anaphase-promoting complex through binding to Cdc20. Results:We identify a new Cdc20-binding motif within BubR1 termed the Phe box. Conclusion: The Phe box maintains steady-state levels of BubR1-containing checkpoint complexes in human cells. Significance: Our study provides key insights into the homeostatic mechanisms of a key spindle checkpoint complex.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
83
0

Year Published

2015
2015
2022
2022

Publication Types

Select...
5
4

Relationship

0
9

Authors

Journals

citations
Cited by 59 publications
(84 citation statements)
references
References 57 publications
1
83
0
Order By: Relevance
“…In principle, differences in the ABBA motif receptor of Cdh1 and Cdc20 could contribute to the distinct specificities of the two APC/C co-activators, although it does not seem to contribute significantly to recognition of most Cdh1 substrates. Interestingly, the ABBA motif recognized by human APC/C Cdc20 more closely resembles the motif recognized by APC/C Cdh1 in budding yeast, and the residues in human Cdc20 that form the ABBA motif receptor are likewise similar to those found in budding yeast Cdh1 (28).…”
Section: Discussionmentioning
confidence: 84%
See 1 more Smart Citation
“…In principle, differences in the ABBA motif receptor of Cdh1 and Cdc20 could contribute to the distinct specificities of the two APC/C co-activators, although it does not seem to contribute significantly to recognition of most Cdh1 substrates. Interestingly, the ABBA motif recognized by human APC/C Cdc20 more closely resembles the motif recognized by APC/C Cdh1 in budding yeast, and the residues in human Cdc20 that form the ABBA motif receptor are likewise similar to those found in budding yeast Cdh1 (28).…”
Section: Discussionmentioning
confidence: 84%
“…These include GXEN in Xenopus Xkid (18), the A box or DAD motif (RXLXPSN) in Xenopus Aurora A (19,20), the O-box (PASPLTEKNAK, essential residues underlined) in Drosophila ORC1 (21), LXEXXXXN in Saccharomyces cerevisiae Spo13 (22), LLK in human Claspin (23), the CRY-box in mouse Cdc20 (24), NKSEN in budding yeast Sgo1 (25), and the ABBA motif, FX(I/V/L)(F/Y/H)X(D/E), in human cyclin A, Bub1, and budding yeast Clb5 (26,27). Only the ABBA motif has a known binding site on the co-activator WD40 domains (12,28). Some alternative degrons resemble D-and KEN-boxes, including RQLF in budding yeast Ase1 (29) and GXEN and NKSEN in XKid and Sgo1, respectively.…”
mentioning
confidence: 99%
“…However, in MCC, and possibly also in BC-1 derived from MCC, a region in the WD-repeat domain of Cdc20 is already bound to BubR1 (28). In BC-2, the internal binding site of BubR1 is also bound to some part of the WD40-repeat domain of Cdc20, but the exact mode of this interaction is not known (29). If chaperonin binds a specific region of the Cdc20 moiety of mitotic checkpoint complexes, this may be in a part not occupied by BubR1 in either BC-1 or BC-2.…”
Section: Discussionmentioning
confidence: 99%
“…The yeast protein Acm1 utilizes a similar motif known as the 'A motif' in addition to its KEN box to interact with and thereby inhibit the CDC20 yeast ortholog Cdh1 (Enquist-Newman et al, 2008;He et al, 2013). Because recent reports have shown the existence of a similar motif in BUBR1 (dubbed the Phe box or ICDC20BD) that is important for a stable BUBR1-CDC20 interaction and for SAC activity (Diaz-Martinez et al, 2015;Lischetti et al, 2014), we tested whether this region in BUB1 is involved in the localization of CDC20 to kinetochores. CDC20 localizes strongly to kinetochores in nocodazole-treated cells, and this depends on BUB1 (Fig.…”
Section: ) Lap-bub1mentioning
confidence: 99%