The cell softening as a universal indicator of cell damage during cytotoxic effects

Murashko, Anton V.; Frolova, Anastasia; Akovantseva, Anastasiya A.; Kotova, Svetlana; Timashev, Peter; Efremov, Yuri M.

doi:10.1016/j.bbagen.2023.130348

Cited by 6 publications

(7 citation statements)

References 58 publications

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“…When studying the mechanical properties of the mucosa-like construct by AFM, no statistical differences in the elastic modulus (Young's modulus) of the cell layer were found. As shown before, changes in mechanical properties of the cell layer are related to their functional state and change during the cytotoxic actions [53]. Nevertheless, there was a tendency for Young's modulus to decrease in the current group compared with the others, which also proves its strong toxic effect on cells.…”

Section: Discussionsupporting

confidence: 57%

Oral Galvanism Side Effects: Comparing Alloy Ions and Galvanic Current Effects on the Mucosa-like Model

Chepelova,

Antoshin,

Voloshin

et al. 2023

JFB

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The interaction of different dental alloys with the oral environment may cause severe side effects (e.g., burning sensation, inflammatory reactions, carcinogenesis) as a result of oral galvanism. However, the pathogenesis of side effects associated with oral galvanism is still unclear, and the effects of direct current and alloy corrosion ions are considered potentially contributing factors. Therefore, the aim of this study was to systemically compare the damaging effects of (1) galvanism as a synergistic process (direct current + corrosion ions), (2) direct current separately, and (3) corrosion ions separately on an in vitro mucosa-like model based on a cell line of immortalized human keratinocytes (HaCaTs) to reveal the factors playing a pivotal role in dental alloys side effects. For this, we chose and compared the dental alloys with the highest risk of oral galvanism: Ti64–AgPd and NiCr–AgPd. We showed that galvanic current may be the leading damaging factor in the cytotoxic processes associated with galvanic coupling of metallic intraoral appliances in the oral cavity, especially in the short-term period (28 days). However, the contribution of corrosion ions (Ni2+) to the synergistic toxicity was also shown, and quite possibly, in the long term, it could be no less dangerous.

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Section: Discussionsupporting

confidence: 57%

Oral Galvanism Side Effects: Comparing Alloy Ions and Galvanic Current Effects on the Mucosa-like Model

Chepelova,

Antoshin,

Voloshin

et al. 2023

JFB

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“…They argued that the method’s strength comes from being able to measure cytotoxicity at the level of single cells. Murashko et al support the idea that cytotoxicity can be measured but found that cytotoxicity overall decreases the stiffness of a population ( Figure 6 ) [ 69 ].…”

Section: Current Results On Cell Stiffnessmentioning

confidence: 90%

“…However, given the popularity of non-AFM methods for cell mechanics, it is entirely possible that other methods-especially those that are already fast-will come to dominate the field. The need for easier and faster measurements has also been pointed out earlier [42,69]. This is partly due to the major lack of good software solutions to easily analyze force data, similar to how Gwyddion has become universal to analyzing topographical AFM images.…”

Section: Discussionmentioning

confidence: 99%

Atomic Force Microscopy for the Study of Cell Mechanics in Pharmaceutics

Siboni,

Ruseska,

Zimmer

2024

Pharmaceutics

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Cell mechanics is gaining attraction in drug screening, but the applicable methods have not yet become part of the standardized norm. This review presents the current state of the art for atomic force microscopy, which is the most widely available method. The field is first motivated as a new way of tracking pharmaceutical effects, followed by a basic introduction targeted at pharmacists on how to measure cellular stiffness. The review then moves on to the current state of the knowledge in terms of experimental results and supplementary methods such as fluorescence microscopy that can give relevant additional information. Finally, rheological approaches as well as the theoretical interpretations are presented before ending on additional methods and outlooks.

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“…On the other hand, a decrease in the Young's modulus of human macrophages was observed upon stimulation with LPS [37]. The results of the latter study, however, can be affected by the high concentration of LPS (10 µg/mL), which induced cytotoxic effects, causing apoptosis and cell softening [70,71]. On the other hand, elasticity of macrophages determining by actin polymerization was demonstrated to affect innate macrophage functions including phagocytosis, secretion of cytokines, and transcriptomic profiles [38].…”

Section: Phagoc Ytosismentioning

confidence: 84%

Radical-Generating Activity, Phagocytosis, and Mechanical Properties of Four Phenotypes of Human Macrophages

Suleimanov,

Efremov,

Klyucherev

et al. 2024

IJMS

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Macrophages are the major players and orchestrators of inflammatory response. Expressed proteins and secreted cytokines have been well studied for two polar macrophage phenotypes—pro-inflammatory M1 and anti-inflammatory regenerative M2, but little is known about how the polarization modulates macrophage functions. In this study, we used biochemical and biophysical methods to compare the functional activity and mechanical properties of activated human macrophages differentiated from monocyte with GM-CSF (M0_GM) and M-CSF (M0_M) and polarized into M1 and M2 phenotypes, respectively. Unlike GM-CSF, which generates dormant cells with low activity, M-CSF confers functional activity on macrophages. M0_M and M2 macrophages had very similar functional characteristics—high reactive oxygen species (ROS) production level, and higher phagocytosis and survival compared to M1, while M1 macrophages showed the highest radical-generating activity but the lowest phagocytosis and survival among all phenotypes. All phenotypes decreased their height upon activation, but only M1 and M2 cells increased in stiffness, which can indicate a decrease in the migration ability of these cells and changes in their interactions with other cells. Our results demonstrated that while mechanical properties differ between M0 and polarized cells, all four phenotypes of monocyte-derived macrophages differ in their functional activities, namely in cytokine secretion, ROS production, and phagocytosis. Within the broad continuum of human macrophages obtained in experimental models and existing in vivo, there is a diversity of phenotypes with varying combinations of both markers and functional activities.

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The cell softening as a universal indicator of cell damage during cytotoxic effects

Cited by 6 publications

References 58 publications

Oral Galvanism Side Effects: Comparing Alloy Ions and Galvanic Current Effects on the Mucosa-like Model

Oral Galvanism Side Effects: Comparing Alloy Ions and Galvanic Current Effects on the Mucosa-like Model

Atomic Force Microscopy for the Study of Cell Mechanics in Pharmaceutics

Radical-Generating Activity, Phagocytosis, and Mechanical Properties of Four Phenotypes of Human Macrophages

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