The cell wall proteome from two strains of Pseudocercospora fijiensis with differences in virulence

Burgos-Canul, Yamily Yazmin; Canto-Canché, Blondy; Berezovski, Maxim V.; Mironov, Gleb G.; Loyola‐Vargas, Víctor M.; Rosa, Ana; Tzec-Simá, Miguel; Brito‐Argáez, Ligia; Carrillo-Pech, Mildred; Grijalva-Arango, Rosa; Muñoz-Pérez, Gilberto; Islas‐Flores, Ignacio

doi:10.1007/s11274-019-2681-2

Cited by 14 publications

(13 citation statements)

References 65 publications

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“…virulent Oz2b strain, signaling that these de novo biosynthetic pathways are important for virulence of this pathogen 25 . Here, another highly expressed enzyme during biotrophy was a putative purine permease, revealing that both routes are functional in P. fijiensis.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Pseudocercospora fijiensis genes upregulated during early interaction with Musa acuminata (var. Dwarf Cavendish)

Manzanero

Carreón-Anguiano

Todd

et al. 2021

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Pseudocercospora fijiensis is a filamentous, hemi[B1] biotrophic fungus whose infection process in banana comprises biotrophic and necrotrophic phases; the biotrophic phase is the longer and less damaging of the two but is nonetheless a crucial stage of fungal establishment in the host. To discover the genes essential in this stage, we conducted an interaction experiment to isolate the transcriptome of the P. fijiensis and Musa acuminata interaction during the first 9 days of infection. Of more than 7000 P. fijiensis genes identified, the fifteen most highly expressed genes (RPKM>500) were analyzed. Specific non-canonical effector candidates were identified following in silico characterization which may be fundamental to pathogenicity. This report reveals essential details of a poorly-elucidated stage of the P. fijiensis-Musa sp. pathosystem.

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Section: Resultsmentioning

confidence: 99%

Analysis of Pseudocercospora fijiensis genes upregulated during early interaction with Musa acuminata (var. Dwarf Cavendish)

Manzanero

Carreón-Anguiano

Todd

et al. 2021

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“…Aliquots of dry rice starch powders were incubated with proteomics-grade trypsin (Sigma-Aldrich, St. Louis, MO, USA) at a ratio of 24 μL:1 mg at 37 °C for 18 h with gentle agitation. The supernatant was collected and analyzed by LC-MS/MS using the following protocol [ 54 ]. Further aliquots were incubated with 50% isopropanol:50 mM NaCl solution for 45 min at room temperature with gentle agitation.…”

Section: Methodsmentioning

confidence: 99%

Three Diverse Granule Preparation Methods for Proteomic Analysis of Mature Rice (Oryza sativa L.) Starch Grain

et al. 2022

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Starch is the primary form of reserve carbohydrate storage in plants. Rice (Oryza sativa L.) is a monocot whose reserve starch is organized into compounded structures within the amyloplast, rather than a simple starch grain (SG). The mechanism governing the assembly of the compound SG from polyhedral granules in apposition, however, remains unknown. To further characterize the proteome associated with these compounded structures, three distinct methods of starch granule preparation (dispersion, microsieve, and flotation) were performed. Phase separation of peptides (aqueous trypsin-shaving and isopropanol solubilization of residual peptides) isolated starch granule-associated proteins (SGAPs) from the distal proteome of the amyloplast and the proximal ‘amylome’ (the amyloplastic proteome), respectively. The term ‘distal proteome’ refers to SGAPs loosely tethered to the amyloplast, ones that can be rapidly proteolyzed, while proximal SGAPs are those found closer to the remnant amyloplast membrane fragments, perhaps embedded therein—ones that need isopropanol solvent to be removed from the mature organelle surface. These two rice starch-associated peptide samples were analyzed using nano-liquid chromatography–tandem mass spectrometry (Nano-HPLC-MS/MS). Known and novel proteins, as well as septum-like structure (SLS) proteins, in the mature rice SG were found. Data mining and gene ontology software were used to categorize these putative plastoskeletal components as a variety of structural elements, including actins, tubulins, tubulin-like proteins, and cementitious elements such as reticulata related-like (RER) proteins, tegument proteins, and lectins. Delineating the plastoskeletal proteome begins by understanding how each starch granule isolation procedure affects observed cytoplasmic and plastid proteins. The three methods described herein show how the technique used to isolate SGs differentially impacts the subsequent proteomic analysis and results obtained. It can thus be concluded that future investigations must make judicious decisions regarding the methodology used in extracting proteomic information from the compound starch granules being assessed, since different methods are shown to yield contrasting results herein. Data are available via ProteomeXchange with identifier PXD032314.

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“…However, the authors found that the percentage of proteins involved in metabolic processes was higher in the less virulent strain, unlike other studies involving Fusarium oxysporum race 4 that found the percentage of proteins was higher in the more virulent strains [ 133 ]. In addition, acid trehalase located in the cell wall was uniquely found in the highly virulent strain [ 134 ].…”

Section: Other Pathogenicity Genesmentioning

confidence: 99%

Genetic Characteristics and Metabolic Interactions between Pseudocercospora fijiensis and Banana: Progress toward Controlling Black Sigatoka

Noar

Thomas

Daub

2022

Plants

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The international importance of banana and severity of black Sigatoka disease have led to extensive investigations into the genetic characteristics and metabolic interactions between the Dothideomycete Pseudocercospora fijiensis and its banana host. P. fijiensis was shown to have a greatly expanded genome compared to other Dothideomycetes, due to the proliferation of retrotransposons. Genome analysis suggests the presence of dispensable chromosomes that may aid in fungal adaptation as well as pathogenicity. Genomic research has led to the characterization of genes and metabolic pathways involved in pathogenicity, including: secondary metabolism genes such as PKS10-2, genes for mitogen-activated protein kinases such as Fus3 and Slt2, and genes for cell wall proteins such as glucosyl phosphatidylinositol (GPI) and glycophospholipid surface (Gas) proteins. Studies conducted on resistance mechanisms in banana have documented the role of jasmonic acid and ethylene pathways. With the development of banana transformation protocols, strategies for engineering resistance include transgenes expressing antimicrobial peptides or hydrolytic enzymes as well as host-induced gene silencing (HIGS) targeting pathogenicity genes. Pseudocercospora fijiensis has been identified as having high evolutionary potential, given its large genome size, ability to reproduce both sexually and asexually, and long-distance spore dispersal. Thus, multiple control measures are needed for the sustainable control of black Sigatoka disease.

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The cell wall proteome from two strains of Pseudocercospora fijiensis with differences in virulence

Cited by 14 publications

References 65 publications

Analysis of Pseudocercospora fijiensis genes upregulated during early interaction with Musa acuminata (var. Dwarf Cavendish)

Analysis of Pseudocercospora fijiensis genes upregulated during early interaction with Musa acuminata (var. Dwarf Cavendish)

Three Diverse Granule Preparation Methods for Proteomic Analysis of Mature Rice (Oryza sativa L.) Starch Grain

Genetic Characteristics and Metabolic Interactions between Pseudocercospora fijiensis and Banana: Progress toward Controlling Black Sigatoka

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