The cellular and molecular mechanisms of vertebrate lens development

Cvekl, Aleš; Ashery‐Padan, Ruth

doi:10.1242/dev.107953

Cited by 194 publications

(192 citation statements)

References 246 publications

(300 reference statements)

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“…12). The lens, cornea and iris are lineage-specific novelties in cephalopods, but interestingly, Pax6 and Six3 are required for lens induction in vertebrates (Ogino et al, 2012;Cvekl and Ashery-Padan, 2014). Currently, little is known about any lens-specific function of eyeless and twin of eyeless (Pax6 orthologs) in Drosophila, but imaginal disc cells expressing eyeless and twin of eyeless that give rise to the retina also generate the lens (Charlton-Perkins et al, 2011a).…”

Section: Correlating Gene Expression Studies With Cell Fatesmentioning

confidence: 99%

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

et al. 2016

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Photoreception is a ubiquitous sensory ability found across the Metazoa, and photoreceptive organs are intricate and diverse in their structure. Although the morphology of the compound eye in Drosophila and the single-chambered eye in vertebrates have elaborated independently, the amount of conservation within the 'eye' gene regulatory network remains controversial, with few taxa studied. To better understand the evolution of photoreceptive organs, we established the cephalopod Doryteuthis pealeii as a lophotrochozoan model for eye development. Utilizing histological, transcriptomic and molecular assays, we characterize eye formation in Doryteuthis pealeii. Through lineage tracing and gene expression analyses, we demonstrate that cells expressing Pax and Six genes incorporate into the lens, cornea and iris, and the eye placode is the sole source of retinal tissue. Functional assays demonstrate that Notch signaling is required for photoreceptor cell differentiation and retinal organization. This comparative approach places the canon of eye research in traditional models into perspective, highlighting complexity as a result of both conserved and convergent mechanisms.

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Section: Correlating Gene Expression Studies With Cell Fatesmentioning

confidence: 99%

Eye development and photoreceptor differentiation in the cephalopod Doryteuthis pealeii

et al. 2016

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“…Mammalian lens development is an advantageous system with which to study the molecular mechanisms of cellular differentiation, including the regulation of cell cycle exit, chromatin dynamics and elimination of subcellular organelles (Bassnett, 2009;Cvekl and Ashery-Padan, 2014). The lens is composed of a layer of epithelial cells that overlie a bulk of differentiated fiber cells.…”

Section: Introductionmentioning

confidence: 99%

Chromatin remodeling enzyme Snf2h regulates embryonic lens differentiation and denucleation

Limi

McGreal

et al. 2016

Development

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Ocular lens morphogenesis is a model for investigating mechanisms of cellular differentiation, spatial and temporal gene expression control, and chromatin regulation. Brg1 (Smarca4) and Snf2h (Smarca5) are catalytic subunits of distinct ATP-dependent chromatin remodeling complexes implicated in transcriptional regulation. Previous studies have shown that Brg1 regulates both lens fiber cell differentiation and organized degradation of their nuclei (denucleation). Here, we employed a conditional Snf2h flox mouse model to probe the cellular and molecular mechanisms of lens formation. Depletion of Snf2h induces premature and expanded differentiation of lens precursor cells forming the lens vesicle, implicating Snf2h as a key regulator of lens vesicle polarity through spatial control of Prox1, Jag1, p27Kip1 (Cdkn1b) and p57Kip2 (Cdkn1c) gene expression. The abnormal Snf2h −/− fiber cells also retain their nuclei. RNA profiling of Snf2h −/− and Brg1 −/− eyes revealed differences in multiple transcripts, including prominent downregulation of those encoding Hsf4 and DNase IIβ, which are implicated in the denucleation process. In summary, our data suggest that Snf2h is essential for the establishment of lens vesicle polarity, partitioning of prospective lens epithelial and fiber cell compartments, lens fiber cell differentiation, and lens fiber cell nuclear degradation.

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“…PAX6 se encuentra ubicado en el cromosoma 11p13 54,55 y se expresa ampliamente en la vesícula óptica, el cristalino, la retina y la córnea durante el desarrollo ocular [56][57][58] . Típicamente, las mutaciones de PAX6 originan aniridia bilateral 59,60 , aunque ciertas mutaciones también se han asociado con un espectro más amplio de defectos oculares y cerebrales 60 .…”

Section: Pax6unclassified