The Changes in Dynamics of Solid Supported Lipid Films Following Hybridization of Short Sequence DNA

Hianik, Tibor; Fajkus, Martin; Sivak, Branislav; Rosenberg, Ivan; Kois, Pavel; Wang, Joseph

doi:10.1002/(sici)1521-4109(200005)12:7<495::aid-elan495>3.3.co;2-5

Cited by 4 publications

(8 citation statements)

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“…However, pressure due to the high electric field across the multibilayers will also be exerted to DMPC. Conformational changes in membranes and electrostriction due to such effects have been reported from membrane capacitance measurements by Sargent (1975a,b), Hianik and Passechnik (1995), Hianik (2000), and Hianik et al (2000). In the latter two cases solid supported membranes have been used that were quite similar to ours.…”

Section: Discussionsupporting

confidence: 69%

“…This explanation is supported by a semiquantitative analysis of the observed dichroic phenomena. Increasing the tilt angle leads to a thinner membrane and might correspond to what was found by capacitance measurements (Hianik, 2000;Hianik et al, 2000). E-ME measurements as described in this paper can be used for an experimental determination of Young's elasticity modulus E ?…”

Section: Discussionsupporting

confidence: 67%

“…On the other hand CH-stretching bands in the E-ME spectra give no evidence for such a conclusion. Electro-striction was suggested based on capacitance measurements of supported lipid membranes (Hianik, 2000;Hianik et al, 2000;Hianik and Passechnik, 1995). To check the consistence of our experimental data and interpretation with results obtained by capacitance measurements, we have calculated the Young's elasticity modulus a DMPC bilayer.…”

Section: Interpretation Of the Electric Field Effect On A Molecular Levelmentioning

confidence: 99%

See 2 more Smart Citations

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Schwarzott

Baurecht

Naumann

et al. 2004

Biophysical Journal

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The effect of electric fields on dry oriented multibilayers of dimyristoylphosphatidylcholine (DMPC) was investigated by transmission Fourier transform infrared electric field modulated excitation (E-ME) spectroscopy. A periodic rectangular electric potential (0-150 V, 1.25 Hz, 28.4 degrees C +/- 0.2 degrees C) was applied across the sample. To discriminate electric field-induced effects from possible temperature-induced effects resulting from a current flow (<1 pA) across the sample, corresponding temperature-modulated excitation (T-ME) measurements within the temperature uncertainty limits of +/-0.2 degrees C at 28.4 degrees C were performed. T-ME induced reversible gauche defects in the hydrocarbon chains, whereas E-ME resulted in reversible compression of dry DMPC bilayers. Periodic variation of the tilt angle of the hydrocarbon chains is suggested. The degree of absorbance modulation in the CH-stretching region was found to be in the order of 1:700, corresponding to a variation of the bilayer thickness of Deltaz = 0.0054 nm. Using a series connection of capacitors as equivalent circuit of the cell resulted in E = (1.2 +/- 0.7) x 10(7) V/m for the electric field in DMPC. Young's elasticity modulus of DMPC could be calculated to be E( perpendicular ) = 2.2 x 10(6) Pa +/- 1.8 x 10(6) Pa, which is in good agreement with published data obtained by electric field-dependent capacitance measurements.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 67%

Section: Interpretation Of the Electric Field Effect On A Molecular Levelmentioning

confidence: 99%

See 1 more Smart Citation

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Schwarzott

Baurecht

Naumann

et al. 2004

Biophysical Journal

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show abstract

“…Once an alkanethiol SAM has been applied to a Cu-deposited mesh, then more complex self-assembled layers can be applied, such as phospholipids, to establish a supported lipid bilayer membrane (s-BLM) [10,28,32,36,[38][39][40]. The IR transmission spectrum of a bare Cu-deposited mesh (top, black trace) is shown in figure 7.…”

Section: Phospholipid Bilayersmentioning

confidence: 99%

Scaffolding for nanotechnology: extraordinary infrared transmission of metal microarrays for stacked sensors and surface spectroscopy

Williams¹,

Rodriguez²,

Teeters-Kennedy³

et al. 2004

Nanotechnology

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Self-assembled monolayers, phospholipid bilayers, and membrane bound proteins are assembled on a subwavelength metallic array. These assemblies are assayed with direct infrared absorption spectroscopy which is greatly enhanced due to the extraordinary infrared transmission of the arrays. Stacking the arrays, one upon another, accentuates the surface plasmon properties and provides the basis of a nanospaced capacitive sensor.

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“…Le Saux et al (2001) report on voltage-induced reorientations of lipid headgroups using dried lipid multilayers of dioleylphosphatidylcholine as a membrane model. I observed the same phenomenon many years ago based on the measurement of capacitance relaxation currents (displacement currents) in black lipid membranes of various compositions (Sargent 1975a,b), and corresponding effects have been seen with virtually solvent-free bilayers (Hianik and Passechnik, 1995;Hianik et al, 2000). The independent observation of the same phenomenon by two completely different methods and in rather different model systems provides good corroboration of the influence of transmembrane voltage on the structure of the lipid headgroup region, but it is of interest to compare the results of the two methods in more detail.…”

mentioning

confidence: 76%

Influence of Transmembrane Voltage on the Structure of Membrane Components

Sargent

2001

Biophysical Journal

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The Changes in Dynamics of Solid Supported Lipid Films Following Hybridization of Short Sequence DNA

Cited by 4 publications

References 0 publications

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Electric Field-Induced Changes in Lipids Investigated by Modulated Excitation FTIR Spectroscopy

Scaffolding for nanotechnology: extraordinary infrared transmission of metal microarrays for stacked sensors and surface spectroscopy

Influence of Transmembrane Voltage on the Structure of Membrane Components

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