The Chaperonin Containing TCP1 Complex (CCT/TRiC) Is Involved in Mediating Sperm-Oocyte Interaction

Dun, Matthew D.; Smith, Nathan D.; Baker, Mark A.; Lin, Mingwei; Aitken, R. John; Nixon, Brett

doi:10.1074/jbc.m110.188888

Cited by 107 publications

(98 citation statements)

References 71 publications

(58 reference statements)

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“…While the confirmation and significance of such targets remain to be validated, many of these substrates may be homologous to those that are activated during the capacitation of mammalian spermatozoa [33]. For instance, molecular chaperones are targeted for tyrosine phosphorylation during the latter stages of capacitation and subsequently play a key role in surface remodelling events that prime spermatozoa for their interaction with the outer vestments of the ovum [20,25,34]. Conversely, both HSPA5 and HSP90AA1 are among a cohort of molecular chaperones that reside on the human sperm surface [35] and form part of the sensing machinery that modulates intracellular calcium concentrations [36], and hence facilitates sperm capacitation.…”

Section: Discussionmentioning

confidence: 99%

“…In situ proximity ligation assays (PLA) were conducted according to manufacturer's instructions (OLINK Biosciences, Uppsala, Sweden; as described by Dun et al [25]) using anti-ODF2 and anti-phosphotyrosine antibodies. Coverslips were mounted as previously described for immunocytochemistry and visualized by fluorescence microscopy (Carl Zeiss, Sydney, Australia).…”

Section: (G) Duolink Proximity Ligation Assaymentioning

confidence: 99%

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The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage

et al. 2016

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Although mammalian spermatozoa only acquire functional maturity as they are conveyed through the male (epididymal maturation) and female (capacitation) reproductive tracts, the degree of post-testicular development necessary to achieve fertilization in other vertebrate species remains far less clear. Indeed, despite reports that the epididymis of birds and reptiles is capable of secreting proteins that bind and modify the sperm surface characteristics, it remains unclear whether capacitation is a pre-requisite for fertilization in these species. Using the ancient reptilian Australian saltwater crocodile as a model, this study was undertaken to explore whether reptile sperm do undergo capacitation-like changes following ejaculation. Our studies revealed that crocodile spermatozoa experienced a rapid and sustained, cyclic-AMP mediated increase in progressive motility following incubation under conditions optimized for the induction of capacitation in mammalian species such as the mouse and human. This response was coupled with elevated levels of phosphorylation associated with both protein kinase A and tyrosine kinase substrates, the latter of which were predominantly localized within the sperm flagellum. In findings that also accord with mammalian spermatozoa, we confirmed a homologue of outer dense fibre 2 as one of the principal substrates for tyrosine phosphorylation. Overall, our findings support the concept that crocodile spermatozoa do undergo a process that is homologous to capacitation in preparation for fertilization of an ovum.

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Section: Discussionmentioning

confidence: 99%

Section: (G) Duolink Proximity Ligation Assaymentioning

confidence: 99%

The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage

et al. 2016

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“…Seminal plasma transferrin correlates with sperm counts and fertility in men (Foresta et al 1986) and lactotransferrin is associated with sperm motility in Asian elephants (Kiso et al 2013). TCP1-containing chaperonins (CCTs) are present on the sperm surface of mice and boars (Belleannée et al 2011) and mediate sperm-oocyte interaction (Dun et al 2011). Interestingly, the presence of CCTs 5 and 8 in bull sperm membrane was negatively correlated with fertility.…”

Section: Proteins Associated With Protection Of Sperm Integrity and Fmentioning

confidence: 99%

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Santos¹,

Sousa²,

Martins³

et al. 2014

Reproduction

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This study was conducted to characterize the major proteins of the peccary seminal plasma, based on the semen samples collected from nine adult and reproductively sound animals. Our approach included the use of two-dimensional electrophoresis followed by Coomassie blue staining and analysis of polypeptide maps with PDQuest Software (Bio-Rad). Proteins were identified by tandem mass spectrometry (LC-MS/MS). We detected 179 protein spots per gel and 98 spots were identified by mass spectrometry, corresponding to 23 different proteins. The combined intensity of those spots accounted for 56.2G6% of the intensities of all spots and 60.9% of the intensities of spots presented in every protein map. Protein spots identified as clusterin represented 19.7G8.3% of the integrated optical densities of all spots detected in the seminal plasma maps. There was a negative association (rZK0.87; P!0.05) between the intensity of a clusterin spot and the percentage of sperm with functional membrane. Spermadhesin porcine seminal plasma protein 1 and bodhesin 2 comprised 5.4G1.9 and 8.8G3.9% of the total intensity of all spots respectively. Many proteins appeared in a polymorphic pattern, such as clusterin (27 spots), epididymal secretory glutathione peroxidase (ten spots), inter-a-trypsin inhibitor (12 spots), and IgG-binding protein (ten spots), among others. In conclusion, we presently describe the major seminal plasma proteome of the peccary, which exhibits a distinct high expression of clusterin isoforms. Knowledge of wild species reproductive biology is crucial for an understanding of their survival strategies and adaptation in a changing environment.

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“…Genes upregulated are significantly enriched for targets displaying testis-specific expression (Figures 1 and 2a; P = 0.001, Fisher's exact test; odds ratio = 1.48; 95% confidence interval = 1.16-1.86), whereas the set of genes downregulated after mating displays a deficit of testisspecific genes (P = 2.2e − 16, Fisher's exact test; odds ratio = 0.16; 95% confidence interval = 0.08-0.28). The set of upregulated genes also includes a strong enrichment for Chaperonin-containing T-complex (GO:0005832; 7 genes, P = 3.40e − 7), a complex that is known to be relevant for spermatogenesis (Soues et al, 2003;Dun et al, 2011). For instance, this category includes the genes T-cp1zeta, T-complex chaperonin 5, sarah and CCT-γ that are involved in meiosis and unfolded protein response.…”

Section: Mating Induces Upregulation Of Testis-specific Gene Expressionmentioning

confidence: 99%

Reproductive activity triggers accelerated male mortality and decreases lifespan: genetic and gene expression determinants in Drosophila

Branco¹,

Schilling

Silkaitis

et al. 2016

Heredity

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Reproduction and aging evolved to be intimately associated. Experimental selection for early-life reproduction drives the evolution of decreased longevity in Drosophila whereas experimental selection for increased longevity leads to changes in reproduction. Although life history theory offers hypotheses to explain these relationships, the genetic architecture and molecular mechanisms underlying reproduction-longevity associations remain a matter of debate. Here we show that mating triggers accelerated mortality in males and identify hundreds of genes that are modulated upon mating in the fruit fly Drosophila melanogaster. Interrogation of genome-wide gene expression in virgin and recently mated males revealed coherent responses, with biological processes that are upregulated (testis-specific gene expression) or downregulated (metabolism and mitochondria-related functions) upon mating. Furthermore, using a panel of genotypes from the Drosophila Synthetic Population Resource (DSPR) as a source of naturally occurring genetic perturbation, we uncover abundant variation in longevity and reproduction-induced mortality among genotypes. Genotypes displayed more than fourfold variation in longevity and reproduction-induced mortality that can be traced to variation in specific segments of the genome. The data reveal individual variation in sensitivity to reproduction and physiological processes that are enhanced and suppressed upon mating. These results raise the prospect that variation in longevity and age-related traits could be traced to processes that coordinate germline and somatic function.

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The Chaperonin Containing TCP1 Complex (CCT/TRiC) Is Involved in Mediating Sperm-Oocyte Interaction

Abstract: Background: Mammalian fertilization is initiated by sperm-zona pellucida (ZP) interactions. Results: The chaperonin-containing TCP1 complex (CCT/TRiC) was identified on the surface of mouse spermatozoa and shown to have an indirect role in ZP adhesion.

Cited by 107 publications

References 71 publications

The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage

The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage

Protein profile of the seminal plasma of collared peccaries (Pecari tajacu Linnaeus, 1758)

Reproductive activity triggers accelerated male mortality and decreases lifespan: genetic and gene expression determinants in Drosophila

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