The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA

Liu, Xin-Yi; Ho, Wen-Yueh; Hung, Wei Jing; Shau, Min‐Da

doi:10.1016/j.biomaterials.2009.08.052

Cited by 39 publications

(22 citation statements)

References 22 publications

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“…The discrepancy in the results at weight ratios between DNA condensation (at 1/1) and size measurement (at 5/1) says that noticeable DNA condensation starts early before smallest polymer/DNA complexes were formed. Since the size of polymer/DNA complexes would be affected by polymer's structural arrangement (including backbone structure of polymers) [16,17] and density distribution of positive charges [18], the size difference in the complexes using PEI 600 in comparison with PU-PEI 600 was observed ( Figure 1B). Therefore, the size of polymer/DNA complexes is more informative for describing the extent of polymer/DNA interactions than DNA electrophoretic mobility.…”

Section: The Gel Retardation and Size Analysis Of Pu-pei 600 /Dna Commentioning

confidence: 99%

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

Hung

Cherng

2015

Polymers

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Abstract:A polyurethane (PU) grafted with small molecular weight polyethylenimine (PEI 600 ) was synthesized. This PU-PEI 600 can assemble DNA via electrostatic interactions into nano-sized polymer/DNA complexes. The complexes exhibited great transfection efficiency in delivering DNA along with a reduced cell toxicity comparing to commercial PEI 25k (M w~2 5,000). In order to establish a system for concurrently delivering two different DNA or RNA molecules for cell reprogramming (e.g., induced pluripotent stem cells) or the necessity of multi-expression (e.g., double knock down), the PU-PEI 600 was further functionalized with maleimide molecules. The novel PU-PEI 600 -maleimide would still effectively interact with assigned DNA and different functions of PU-PEI 600 -maleimide/DNA complexes were self-conjugated in presence of a dithiol molecule (1,6-hexanedithiol). In this study, two reporter genes (pEGFP-C2 and pLanRFP-N) were used and evidence of green/red fluorescence co-expression in cells was observed. This article brings a new concept and a practical method for a plurality of different DNA molecules that are more efficient to be concurrently delivered and co-expressed. This method is very helpful in studying cellular multi-regulation or in the treatment of disease with multiple gene defects in vivo.

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Section: The Gel Retardation and Size Analysis Of Pu-pei 600 /Dna Commentioning

confidence: 99%

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

Hung

Cherng

2015

Polymers

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“…When combined with PEI, cationic polyurethane (PU)-shortbranch PEI (PU-PEI) was shown to exhibit high transfection efficiency and low cytotoxicity in vitro and in vivo [149,153]. Using PU-PEI as a delivery vehicle, Yang and colleagues reported efficient delivery of miR-145 to CD133 + GBM cells, which resulted in a significant decrease in their tumorigenic potential and facilitated differentiation into CD133-negative cells [149].…”

Section: Therapeutic Modulation Of Mirnasmentioning

confidence: 99%

MicroRNAs as Molecular Targets for Cancer Therapy: On the Modulation of MicroRNA Expression

Costa

Lima

2013

Pharmaceuticals

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The discovery of small RNA molecules with the capacity to regulate messenger RNA (mRNA) stability and translation (and consequently protein synthesis) has revealed an additional level of post-transcriptional gene control. MicroRNAs (miRNAs), an evolutionarily conserved class of small noncoding RNAs that regulate gene expression post-transcriptionally by base pairing to complementary sequences in the 3' untranslated regions of target mRNAs, are part of this modulatory RNA network playing a pivotal role in cell fate. Functional studies indicate that miRNAs are involved in the regulation of almost every biological pathway, while changes in miRNA expression are associated with several human pathologies, including cancer. By targeting oncogenes and tumor suppressors, miRNAs have the ability to modulate key cellular processes that define the cell phenotype, making them highly promising therapeutic targets. Over the last few years, miRNA-based anti-cancer therapeutic approaches have been exploited, either alone or in combination with standard targeted therapies, aiming at enhancing tumor cell killing and, ideally, promoting tumor regression and disease remission. Here we provide an overview on the involvement of miRNAs in cancer pathology, emphasizing the mechanisms of miRNA regulation. Strategies for modulating miRNA expression are presented and illustrated with representative examples of their application in a therapeutic context.

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“…The polymer exhibited remarkably higher transfection activity in MeWo cells as compared with PEI (25 kDa) although molecular weight of obtained polymer is not much high (MW = 2,800 Da). Liu et al synthesized poly (ester-co-urethane)-PEI conjugate as the degradable PEI through aminolysis of poly (ester-courethane) by LMW PEI (800 Da) [64]. The polymer has two degradable linkages: one is ester bond in the main chain and the other is carbamate one in the side chain.…”

Section: Carbamate Linkagementioning

confidence: 99%

Design and Development of Degradable Polyethylenimines for Delivery of DNA and Small Interfering RNA: An Updated Review

Cho

2012

ISRN Materials Science

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Polyethylenimine (PEI), considered as the most potent and promising alternative carrier to viral vectors, has been studied as the “state of the art” among various polymers for nonviral gene delivery applications for many years. Although PEI-based carrier minimizes the bottlenecks associated with viral vectors such as unwanted immunogenicity and production problems, the toxic side effects of PEI prevent its rapid advancements due to nondegradable nature. In this regard, various degradable cross-linking and/or grafting agents have been linked to synthesize degradable PEIs in order to minimize the toxicity and improve the efficacy of PEI-mediated gene carriers. This paper describes an update on various cross-linkers and grafting agents in the design and development of degradable PEI derivatives and their potential applications for effective delivery of DNAin vitroandin vivo. The molecular weight (MW) of PEI and the structural relationship to its cellular toxicity and transfection ability were also discussed. Finally, the potential applications of various degradable PEIs for small interfering RNA (siRNA)-mediated gene silencing were also covered.

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The characteristics and transfection efficiency of cationic poly (ester–co–urethane) – short chain PEI conjugates self-assembled with DNA

Cited by 39 publications

References 22 publications

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

Maleimide-Functionalized PEI600 Grafted Polyurethane: Synthesis, Nano-Complex Formation with DNA and Thiol-Conjugation of the Complexes for Dual DNA Transfection

MicroRNAs as Molecular Targets for Cancer Therapy: On the Modulation of MicroRNA Expression

Design and Development of Degradable Polyethylenimines for Delivery of DNA and Small Interfering RNA: An Updated Review

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