The characteristics, cellular uptake and intracellular trafficking of nanoparticles made of hydrophobically-modified chitosan

Chiu, Ya Ling; Ho, Yi Cheng; Chen, Yu Ming; Peng, Shu‐Fen; Ke, Cherng-Jyh; Chen, Ko Jie; Mi, Fwu Long; Sung, Hsing‐Wen

doi:10.1016/j.jconrel.2010.05.023

Cited by 192 publications

(120 citation statements)

References 46 publications

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“…The br-BSANPs were prepared by a desolvation-chemical cross-linking technique 19 and the effect of pH and the BSA:brucine ratio were optimized. Typically, BSA was first dissolved in deionized water and the solution was adjusted to the desired pH using 0.1 mol/L sodium hydroxide.…”

Section: Preparation Of Br-bsanpsmentioning

confidence: 99%

Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Chen¹,

Chen²,

Wu³

et al. 2013

IJN

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Section: Preparation Of Br-bsanpsmentioning

confidence: 99%

Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Chen¹,

Chen²,

Wu³

et al. 2013

IJN

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“…Firstly, endocytosis must be distinguished from non-endocytic pathways. The most direct way to determine if the complexes are being endocyted is to use the energy depletion method (lowering the incubation temperature of the cells to 4 °C), since most endocytic pathways are energy dependent [57,63]. To distinguish the phagocytic and macropinocytic pathways from CME and CvME, the most commonly used inhibitors are amiloride (inhibitors of sodium-proton spongue) and wortmannin (inhibitor of phosphoinositide).…”

Section: Tools For the Study Of Uptake Pathwaysmentioning

confidence: 99%

“…CME could be inhibited by using hypertonic medium, cytosol acidification or chlorpromazine drug. According to CvME, genestein, nystatin and filipin are the most commonly used specific inhibitors [57,63,64]. Inhibitors for the study of intracellular fates of complexes are also available.…”

Section: Tools For the Study Of Uptake Pathwaysmentioning

confidence: 99%

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

et al. 2014

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Non-viral gene delivery vectors are emerging as a safer alternative to viral vectors. Among natural polymers, chitosan (Ch) is the most studied one, and low molecular weight Ch, specifically, presents a wide range of advantages for non-viral pDNA delivery. It is crucial to determine the best process for the formation of Low Molecular Weight Chitosan (LMWC)-pDNA complexes and to characterize their physicochemical properties to better understand their behavior once the polyplexes are administered. The transfection efficiency of Ch based polyplexes is relatively low. Therefore, it is essential to understand all the transfection process, including the cellular uptake, endosomal escape and nuclear import, together with the parameters involved in the process to improve the design and development of the non-viral vectors. The aim of this review is to describe the formation and characterization of LMWC based polyplexes, the in vitro transfection process and finally, the in vivo applications of LMWC based polyplexes for gene therapy purposes.

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“…It involves specialized proteins-clathrin and caveolin [13,14]. To identify the endocytic pathway that is involved in the uptake of 4SPCL-PEG-Folic micelles, cells were incubated with chlorpromazine to inhibit clathrin-mediated endocytosis; with MβCD to inhibit caveolae/lipid-raft mediated endocytosis; Cytochalasin D to inhibit macropinocytosis, and with phagocytosis and folic acid to inhibit FR-mediated endocytosis [13,14,33]. From the results thus obtained, Cytochalasin D did not influence the uptake of 4SPCL-PEG-Folic micelles by either KB or MCF-7 cells.…”

Section: Endocytosis Pathwaymentioning

confidence: 99%

Endocytosis Pathways of the Folate Tethered Star-Shaped PEG-PCL Micelles in Cancer Cell Lines

Nguyen

Hsieh

2014

Polymers

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This study reports on the cellular uptake of folate tethered micelles using a branched skeleton of poly(ethylene glycol) and poly(ε-caprolactone). The chemical structures of the copolymers were characterized by proton nuclear magnetic resonance spectroscopy, and Fourier transform infrared spectroscopy. Doxorubicin (DOX) was utilized as an anticancer drug. The highest drug loading efficiencies of DOX in the folate decorated micelle (DMCF) and folate-free micelle (DMC) were found to be 88.5% and 88.2%, respectively, depending on the segment length of the poly(ε-caprolactone) in the copolymers. A comparison of fluorescent microscopic images of the endocytosis pathway in two cell lines, human breast cancer cells (MCF-7) and human oral cavity carcinoma cells (KB), revealed that the micelles were engulfed by KB and MCF-7 cells following in vitro incubation for one hour. Flow cytometric analysis revealed that free folic acid can inhibit the uptake of DOX by 48%-57% and 26%-39% in KB cells and MCF-7 cells, respectively. These results prove that KB cells are relatively sensitive to folate-tethered micelles. Upon administering methyl-β-cyclodextrin, an inhibitor of the caveolae-mediated endocytosis pathway, the uptake of DOX by KB cells was reduced by 69% and that by MCF-7 cells was reduced by 56%. This finding suggests that DMCF enters cells via multiple pathways, OPEN ACCESSPolymers 2014, 6 635 thus implying that the folate receptor is not the only target of tumor therapeutics.

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The characteristics, cellular uptake and intracellular trafficking of nanoparticles made of hydrophobically-modified chitosan

Cited by 192 publications

References 46 publications

Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Hyaluronic acid-coated bovine serum albumin nanoparticles loaded with brucine as selective nanovectors for intra-articular injection

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

Endocytosis Pathways of the Folate Tethered Star-Shaped PEG-PCL Micelles in Cancer Cell Lines

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