The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus

Azad, Ahmed A.; Barrett, Susan; Fahey, K. J.

doi:10.1016/0042-6822(85)90094-7

Cited by 189 publications

(93 citation statements)

References 26 publications

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“…IBDV belongs to the genus Avibirnavirus of the family Birnaviridae and has two segments of double-stranded genomic RNAs (A and B) (7). Segment B encodes VP1 (97 kDa), a RNAdependent RNA polymerase (8), affecting viral replication and virulence (2,9,10).…”

Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Lin

Zhang

et al. 2015

Journal of Biological Chemistry

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Background: IBDV VP5 protein induces apoptosis in DF-1 cells via interaction with VDAC2. Results: RACK1 inhibits IBDV-induced apoptosis, enhances viral replication, and interacts with both VDAC2 and VP5. Conclusion: RACK1 forms a complex with VDAC2 and VP5, affecting IBDV-induced apoptosis and viral replication. Significance: RACK1 inhibits apoptosis via interaction with VDAC2, indicating sequestration of RACK1 and VDAC2 by VP5 during IBDV infection.

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Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Lin

Zhang

et al. 2015

Journal of Biological Chemistry

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“…The large segment encodes a polyprotein that is processed into 3 structural proteins, VP2, VP3, and VP4. 2,10,19 The large segment also encodes the structural protein VP5 in another reading frame. 15 Of these different genes, VP2 gene encodes for the major antigenic protein and its hypervariable region is what confers variability among different IBDV strains.…”

Section: Introductionmentioning

confidence: 99%

Detection of Infectious Bursal Disease virus from Formalin-Fixed Paraffin-Embedded Tissue by Immunohistochemistry and Real-Time Reverse Transcription-Polymerase Chain Reaction

2007

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Abstract. Formalin fixed paraffin embedded tissues blocks are used routinely to diagnose the economically important immunosuppressive infectious bursal disease virus (IBDV) in chickens. Immunohistochemical detection of viruses in tissue blocks has been done with varying results between laboratories. Extraction of IBDV RNA from tissue blocks allows IBDV strain identification at a molecular level. This allows correlation between virus identity and histological lesions present in the tissue. Experimentally reverse transcription-polymerase chain reaction (RT-PCR) detectable IBDV RNA could always be extracted from tissue blocks with acute +3 or higher histological lesion scores. However, many blocks from diagnostic field cases did not yield detectable IBDV RNA, in spite of having severe IBDV histological lesion scores. The reason for this can be the effect different formalin fixation conditions have on RNA detection from tissue blocks. To study the effect of various fixation parameters on RNA extraction and immunohistochemical detection of IBDV, bursas with maximum histological lesion score of 4 for IBDV were fixed in formalin under various conditions (different pH levels, temperatures, concentrations of formalin, and fixation duration). Only tissues fixed in formalin with a pH of 7.0, concentration of 5 or 10% formaldehyde, storage temperature of 25uC or less, and kept for up to 2 weeks in formalin yielded detectable IBDV RNA upon extraction. No RNA could be detected from tissues fixed under extreme temperature, pH, or formalin concentrations. Optimal fixation conditions for IHC detection of IBDV were 10% formalin concentration, pH 7.0, and temperature of 4uC, where maximum intensity of immunostaining was observed.

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“…Genome segment B (2.9 kb) encodes a 90-kDa protein, VP1 [1], which is a dsRNA polymerase [18,25], and the larger segment, A (3.2 kb), encodes viral proteins VP2, VP3 and VP4 [1], which are produced by autoproteolysis of a 106-kDa precursor polyprotein [2,11,14], and a nonstructural protein, VP5, in another open reading frame [19].…”

mentioning

confidence: 99%

Nucleotide Sequence Analysis of VP2 Hypervariable Domain of Infectious Bursal Disease Virus Detected in Japan from 1993 to 2004

Yamaguchi

Kasanga

Terasaki

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Bursae of Fabricius were collected from 20 chickens diagnosed with infectious bursal disease virus (IBDV) infection from 15 prefectures in 1993 to 2004. Here we report the nucleotide sequence analysis of VP2 hypervariable domain of IBDV genome detected by reverse transcription-polymerase chain reaction from these samples. Ten sequences derived from 10 prefectures in 1996 to 2003 were of the classical type and other 10 sequences derived from 6 prefectures in 1993 to 2004 were of the highly virulent type. Of the classical type sequences, 9 sequences were closely related to the sequence of classical attenuated vaccines used in Japan. Furthermore, two were identical to the sequence of B-Chi5 which represents Vaccine B passaged 5 times in chickens and was reported to be reverted the virulence during the passages. The 10 highly virulent type sequences were classified into four sequences, none of which had been previously detected in Japan. However, the deduced amino acid sequences were identical to each other and to the sequences of highly virulent IBDVs previously detected in Japan. The most common nucleotide sequences, which accounted for 6 of the sequences, were identical to 34 highly virulent type sequences detected in various countries in BLAST search. This is the first report of detection of the sequence in Japan which is identical to highly virulent strains detected in other countries. These findings show the prevalence of classical IBDVs closely related to the attenuated vaccines and highly virulent IBDVs derived from other countries throughout Japan since 1993.

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The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus

Cited by 189 publications

References 26 publications

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Detection of Infectious Bursal Disease virus from Formalin-Fixed Paraffin-Embedded Tissue by Immunohistochemistry and Real-Time Reverse Transcription-Polymerase Chain Reaction

Nucleotide Sequence Analysis of VP2 Hypervariable Domain of Infectious Bursal Disease Virus Detected in Japan from 1993 to 2004

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