The characterization of a full-thickness excision open foot wound model in n5-streptozotocin (STZ)-induced type 2 diabetic rats that mimics diabetic foot ulcer in terms of reduced blood circulation, higher C-reactive protein, elevated inflammation, and reduced cell proliferation

Yu, Caroline Oi-Ling; Leung, Kwok-Sui; Fung, Kwok‐Pui; Lam, F.; Ng, E.S.K.; Lau, Ken; Chow, Simon Kwoon‐Ho; Cheung, Wing-Hoi

doi:10.1538/expanim.17-0016

Cited by 19 publications

(10 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the authors, the main disadvantage of their study is that the ischemia is caused by transient deprival of blood perfusion due to a disruption in blood supply and not due to atherosclerosis and microangiopathy as is seen in humans. Yu et al 22 presented a novel method of creating full thickness wounds on the footpads of diabetic animals.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, we lack information regarding tissue perfusion in the follow‐up period. Yu et al 22 spotted almost fourfold lower blood flow in diabetic wounds on day 13. Tur et al 25 used laser Doppler velocimetry to determine vascular perfusion of ischemic wounds.…”

Section: Discussionmentioning

confidence: 99%

“…The aim of a study by Yu et al 22 was to investigate the influence of DM on ischemic tissue in n5-streptozotocin-induced type 2 DM rats.…”

Section: Yu Et Almentioning

confidence: 99%

See 2 more Smart Citations

Animal experimental models of ischemic wounds – A review of literature

Lovasova

Bém

Chlupáč

et al. 2022

Wound Repair Regeneration

View full text Add to dashboard Cite

Critical limb ischemia is a serious form of peripheral arterial disease (PAD). The consequences of lower limb ischemia are pain, claudication and chronic non‐healing wounds. Patients with diabetes are especially at a high risk for developing non‐healing ulcers. The most serious complication is major amputation. For this reason, there is a significant medical requirement to develop new therapies in order to prevent the progression of PAD. For research purposes, it is crucial to find an appropriate model of chronic ischemia to explore the processes of wound healing. According to recently acquired information, rodents are currently the most commonly used animals in these types of studies. The main advantage of using small animals is the low financial cost due to the relatively small demand for food, water and living space. The disadvantage is their anatomy, which is different from that of humans. Larger animals have a more human‐like anatomy and physiology, but they require more expense and space for housing. A bipedicle skin flap and its modifications are popular models for ischemic wounds. In order to secure healing through re‐epithelisation, as opposed to contraction in rodents, there is a need to remove the panniculus carnosus muscle. Wounds in other experimental animals heal primarily through re‐epithelisation. The application of a silicone mesh underneath the flap prevents vascular regrowth in ischemic tissue. There is an ongoing effort to create in vivo diabetic models for chronic ulcer research. This work presents an overview of existing animal models of ischemic wounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Animal experimental models of ischemic wounds – A review of literature

Lovasova

Bém

Chlupáč

et al. 2022

Wound Repair Regeneration

View full text Add to dashboard Cite

show abstract

“…After the stabilization period, rats were anesthetized using ketamine and xylazine (Ketamine 60 mg/kg and xylazine at 10 mg/kg both given by intraperitoneal route), and a full-thickness wound of 6 mm diameter (excision model) was made using punch biopsy (Ribbels dermal biopsy punch) (Trujillo et al 2015 ; Yu et al 2017 ) on the dorsal side of the palm of the rat. The treatment group of rats were applied Botroclot solution (1–2 drops daily) on the wound with the help of the applicator inbuilt into the bottle until the day of sacrifice.…”

Section: Methodsmentioning

confidence: 99%

Botrops derived hemocoagulase formulation a probable agent for diabetic wound healing

et al. 2020

View full text Add to dashboard Cite

Botroclot is a marketed preparation containing hemocoagulase, which is an enzyme having coagulant activity, isolated from the snake Botrops atrox. This formulation is used in dental surgeries and other minor surgical wounds. However, the formulation remains untested in diabetic wounds. Hence, we proposed a study for the topical application of Botroclot in high-fat diet (HFD) + Streptozotocin (STZ) induced diabetic rats. HFD was fed initially to rats which facilitates the development of insulin resistance. Thereafter, an injection of STZ (40 mg/kg, i.p.) was given. This resulted in the development of diabetes with elevated fasting glucose and impaired glucose tolerance. After stabilization of blood glucose values, wounds were created by punch biopsy on the dorsal side of the palm of the rat to mimic the diabetic wounds frequently seen in the case of humans. Later, the application of Botroclot on these wounds was carried out for 15 days. Topical application of hemocoagulase improved the wound closure and there was a gradual decrease in inflammatory markers and a substantial increase in collagen deposition occurred. Histopathological findings indicated the same, with an increase in granulation tissue suggesting that the topical application moderately improves the wound healing in diabetic rats. We conclude that Botroclot can have a mild to moderate effect in improving collagen deposition and thus wound contraction, improving wound closure in diabetic wounds in rats. This study also establishes the basis for exploration of agents from venom-based sources in diabetic wound healing.

show abstract

“…A well-described model was used, by inducing type 1 diabetes in C57BL6/J mice with a single streptozotocine injection. [35][36][37] Excision wounds were dressed with or without graphene for 12 days. In this model, healing was indeed slower, and exudates were more voluminous.…”

Section: Delayed Modelmentioning

confidence: 99%

Monolayer graphene-on-polymer dressings promote healing and stabilize skin temperature on acute and chronic wound models

Gall

Serantoni

Louche

et al. 2021

Preprint

View full text Add to dashboard Cite

Monolayer graphene directly presented on the wound bed is assessed for its healing properties using both in vitro and in vivo models. For in vivo study, a cutaneous excisional wound is created on the dorsal surface of healthy and type-1 diabetic mice to mimic acute and delayed wound healing, respectively. A pig model is also chosen for its resemblance to human skin. Photographic and histological assessment of the wound are coupled with thermographic data recorded with an infrared camera. Graphene monolayer accelerates early phases of wound healing in vivo in every tested model. Upon removal of the bandage, wounds coated with graphene are less prone to temperature drop compared to the control samples. We hypothesize that graphene may directly shorten the inflammatory phase and/or enhance angiogenesis and cell migration in proliferative phase as demonstrated in vitro. Thermographic assessment of wounds could be of particular interest to follow both phenomena in an objective, rapid and non-invasive manner.

show abstract

The characterization of a full-thickness excision open foot wound model in n5-streptozotocin (STZ)-induced type 2 diabetic rats that mimics diabetic foot ulcer in terms of reduced blood circulation, higher C-reactive protein, elevated inflammation, and reduced cell proliferation

Cited by 19 publications

References 50 publications

Animal experimental models of ischemic wounds – A review of literature

Animal experimental models of ischemic wounds – A review of literature

Botrops derived hemocoagulase formulation a probable agent for diabetic wound healing

Monolayer graphene-on-polymer dressings promote healing and stabilize skin temperature on acute and chronic wound models

Contact Info

Product

Resources

About