The chemical changes in the total antioxidant status and biological activity of GSTP1 polymorphism on nasopharyngeal carcinoma patients

Abstract

Oxidative stress is associated with cancer, including NPC, which is developed by the relation of genetic alteration, EBV infection, and environmental risk. Ile/Val genotype of GSTP1 polymorphism decrease enzyme functions and result in lower total antioxidant status. This study aimed to identify the association of GSTP1 polymorphism and the TAS level. This was a cross-sectional design study. There were 29 NPC patients with their blood as the samples of the study. Blood as the samples for identifying GSTP1 polymorphism by PCR-RFLP-electrophoresis method and measurement of TAS using the ELISA method. There were 21 (72.4%) NPC patients with Ile/Val polymorphism and lower TAS. We found there was no significant association of GSTP1 polymorphism and TAS. Future research with larger samples and adding data about environmental risk such as smoking, alcohol consumption, and toxic substances exposure or multivitamin consumption of the patients can reveal more definitive results. The study may be used as references for determining antioxidant therapy in NPC as well as prevention. The identifying genetic risk factor may help the prevention of NPC.

Role of Glutathione S-transferase Mu 1 and Glutathione S-transferases Theta 1 Polymorphism in the Risk of Developing Type 2 Diabetes Mellitus at Universitas Sumatera Utara Hospital, Medan

“…Several populations in the world showed that GSTM1 gene null polymorphism and/or GSTT1 had the risk of type 2 DM (T2DM) compared with healthy control group [14], [15]. GSTT1 dan GSTM1 gene polymorphism and some diseases have been studied in Indonesia [16], [17], [18], [19], however, so far not research on T2DM. Therefore, this study was aimed to determine the role of GSTM1 and GSTT1 gene polymorphism in T2DM at Universitas Sumatera Utara Hospital, Medan, Indonesia.…”