The chick chorioallantoic membrane as a novel <i>in vivo</i> model for the testing of biomaterials

Valdes, Thelma I.; Kreutzer, Donald L.; Moussy, Francis

doi:10.1002/jbm.10152

Cited by 176 publications

(157 citation statements)

References 23 publications

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“…26,27 Fertilized chicken eggs were incubated at 37°C with ~60% humidity. On day 7 post-fertilization, eggs were first sterilized by immersing in the benzalkonium bromide solution, and then a small window with diameter of ~1.5 cm at the top of the chicken egg shell was made by cutting the shell with a grinding wheel.…”

Section: In Vivo Biocompatibility Of Egels In a Chorioallantoic Membrmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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Injectable electroactive hydrogels (eGels) are promising in regenerative medicine and drug delivery, however, it is still a challenge to obtain such hydrogels simultaneously possessing other properties including uniform structure, degradability, robustness, and biocompatibility. An emerging strategy to endow hydrogels with desirable properties is to incorporate functional nanoparticles in their network. Herein, we report the synthesis and characterization of an injectable hydrogel based on oxidized alginate (OA) crosslinking gelatin reinforced by electroactive tetraaniline-graft-OA nanoparticles (nEOAs), where nEOAs are expected to impart electroactivity besides reinforcement without significantly degrading the other properties of hydrogels. Assays of transmission electron microscopy, 1 H nuclear magnetic resonance, and dynamic light scattering reveal that EOA can spontaneously and quickly self-assemble into robust nanoparticles in water, and this nanoparticle structure can be kept at pH 3~9. Measurement of the gel time by rheometer and the stir bar method confirms the formation of the eGels, and their gel time is dependent on the weight content of nEOAs. As expected, adding nEOAs to hydrogels does not cause the phase separation (scanning electron microscopy observation), but it improves mechanical strength up to ~8 kPa and conductivity up to ~10 −6 S/cm in our studied range. Incubating eGels in phosphate-buffered saline leads to their further swelling with an increase of water content <6% and gradual degradation. When growing mesenchymal stem cells on eGels with nEOA content ≤14%, the growth curves and morphology of cells were found to be similar to that on tissue culture plastic; when implanting these eGels on a chick chorioallantoic membrane for 1 week, mild inflammation response appeared without any other structural changes, indicating their good in vitro and in vivo biocompatibility. With injectability, uniformity, degradability, electroactivity, relative robustness, and biocompatibility, these eGels may have a huge potential as scaffolds for tissue regeneration and matrix for stimuli responsive drug release.

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Section: In Vivo Biocompatibility Of Egels In a Chorioallantoic Membrmentioning

confidence: 99%

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Wang¹,

Wang²,

Teng³

2016

IJN

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“…The hen's embryo, more precisely the chorioallantoic membrane, is as an alternative to the Draize eye rabbits test for the evaluation of ocular formulations. The HET-CAM is a simple, fast and cheap test that provides measurable indices of the biocompatibility of a material [46,47]. The micellar systems tested did not induce haemorrhage, lysis or coagulation both before and after being loaded with ETOX ( figure 1a).…”

Section: Hen's Egg Test-chorioallantoic Membrane Assaymentioning

confidence: 99%

Single and mixed poloxamine micelles as nanocarriers for solubilization and sustained release of ethoxzolamide for topical glaucoma therapy

Ribeiro

Sosnik

Chiappetta

et al. 2012

J. R. Soc. Interface.

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Polymeric micelles of single and mixed poloxamines (Tetronic) were evaluated regarding their ability to host the antiglaucoma agent ethoxzolamide (ETOX) for topical ocular application. Three highly hydrophilic varieties of poloxamine (T908, T1107 and T1307) and a medium hydrophilic variety (T904), possessing a similar number of propylene oxide units but different contents in ethylene oxide, were chosen for the study. The critical micellar concentration and the cloud point of mixed micelles in 0.9 per cent NaCl were slightly greater than the values predicted from the additive rule, suggesting that the co-micellization is hindered. Micellar size ranged between 17 and 120 nm and it was not altered after the loading of ETOX (2.7-11.5 mg drug g -1 poloxamine). Drug solubilization ability ranked in the order: T904 (50-fold increase in the apparent solubility) . T1107 ffi T1307 . T908. Mixed micelles showed an intermediate capability to host ETOX but a greater physical stability, maintaining almost 100 per cent drug solubilized after 28 days. Furthermore, the different structural features of poloxamines and their combination in mixed micelles enabled the tuning of drug release profiles, sustaining the release in the 1-5 days range. These findings together with promising hen's egg test-chorioallantoic membrane biocompatibility tests make poloxamine micelles promising nanocarriers for carbonic anhydrase inhibitors in the treatment of glaucoma.

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“…The chorioallantoic membrane (CAM) assay, used for more than half a century, has become a mainstay in the study of blood vessel development (Auerbach et al, 2003;Cruz et al, 2000;Cruz et al, 1997;DeFouw and DeFouw, 2000a;DeFouw and DeFouw, 2000b;Djonov et al, 2000a;Djonov et al, 2000b;McDonnell et al, 2005;Rizzo and DeFouw, 1996;Rizzo et al, 1995;Rizzo et al, 1993). The CAM assay has also proved useful for biological devices and in tissue engineering applications, particularly those concerning biocompatibility studies and angiogenic responses to tissue-engineered constructs (Borges et al, 2003a;Borges et al, 2003b;Nguyen et al, 1994;Ribatti et al, 2001;Rickert et al, 2003;Valdes et al, 2003;Valdes et al, 2002;Wong et al, 2003). In this work we extend the CAM assay in the development of a histological technique to specifically examine direct vascular invasion into fibrin-based constructs.…”

Section: Introductionmentioning

confidence: 99%

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

Smith

Melhem

Magge

et al. 2007

Microvascular Research

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Using the chick chorioallantoic membrane assay (CAM) and a novel histological technique we investigated the ability of blood vessels to directly invade fibrin-based scaffolds. In our initial experiments utilizing vascular endothelial growth factor (VEGF 165 ) we found no direct invasion. Instead, the fibrin was completely degraded and replaced with highly vascularized new tissue. Addition of fibroblast growth factor-2 (FGF-2), bone morphogenic protein-2 (BMP-2), or plateletderived growth factor-BB (PDGF-BB) to the fibrin construct also did not result in construct vascularization. Because natural and regenerating tissues exhibit complex extracellular matrices (ECMs), we hypothesized that a more complex scaffold may improve blood vessel invasion. Addition of fibronectin, hyaluronic acid, and collagen type I within 20 mg/mL fibrin constructs resulted in no significant improvement. However, the same additive concentrations within 10 mg/ mL fibrin constructs resulted in dramatic improvements, specifically with hyaluronic acid. Overall, we believe these results indicate the importance of structural and functional cues of not only in the initial scaffold but also as the construct is degraded and remodeled. Furthermore, the CAM assay may represent a useful model for understanding ECM interactions as well as for screening and designing tissue engineered scaffolds.

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The chick chorioallantoic membrane as a novel in vivo model for the testing of biomaterials

Cited by 176 publications

References 23 publications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Injectable, degradable, electroactive nanocomposite hydrogels containing conductive polymer nanoparticles for biomedical applications

Single and mixed poloxamine micelles as nanocarriers for solubilization and sustained release of ethoxzolamide for topical glaucoma therapy

Improved growth factor directed vascularization into fibrin constructs through inclusion of additional extracellular molecules

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