The chick embryo chorioallantoic membrane as a bioassay for angiogenesis factors: Reactions induced by carrier materials

Jakob, W.; Jentzsch, K.D.; Mauersberger, B.; Heder, G.

doi:10.1016/s0014-4908(78)80064-7

Cited by 48 publications

(33 citation statements)

References 7 publications

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“…The most commonly used in vivo assays are the corneal micropocket assay, the chorioallantoic membrane (CAM) assay, and the dorsal skin-fold assay (Fournier et al, 1981;Jakob et al, 1978;Kenyon et al, 1996). The corneal micropocket assay, an analysis conducted within an avascular environment, is often used to study the efficacy of angiogenic compounds.…”

Section: Discussionmentioning

confidence: 99%

Angiogenesis and Fibroblast Growth Factors (FGFs) in a Three-Dimensional Collagen Gel Culture

Nagatoro

Fujita

Murata

et al. 2003

Okajimas Folia Anat. Jpn.

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Summary: Small pieces of mouse aorta were cultured in collagen gels, and the formation of capillary-like tubes from the aortic explant was observed under phase-contrast and transmission electron microscopes. Migration of fibroblastic cells from the aortic explant occurred in 2 days. After about 10 days of culture, capillary-like tubes from the aortic explant were formed in the collagen gels. An immunohistochemical study on the collagen gel culture revealed the expression of fibroblast growth factor 2 (FGF-2) near the aortic explant at the active initial stage, with random migration of fibroblastic cells expressing FGF-9. mRNA was isolated from these cultures, and reverse transcription-polymerase chain reaction (RT-PCR) of the cultures revealed the expressions of both FGF-2 and FGF-9. Based on our results, we propose that FGF-9 is also related to angiogenic events.

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Section: Discussionmentioning

confidence: 99%

Angiogenesis and Fibroblast Growth Factors (FGFs) in a Three-Dimensional Collagen Gel Culture

Nagatoro

Fujita

Murata

et al. 2003

Okajimas Folia Anat. Jpn.

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“…The main limitation of CAM assays is the nonspecific inflammatory reactions that may develop as a result of grafting, inducing a secondary vasoproliferative response and impeding quantification of the primary response (Jakob et al, 1978;Spanel-Burowksi et al, 1988). Investigation of histological CAM sections would help to detect the presence of a perivascular inflammatory infiltrate, together with a hyperplastic reaction, if any, of the chorionic epithelium.…”

Section: Limitation Of the Cam Assaymentioning

confidence: 99%

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

et al. 2001

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The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis. Anat Rec 264: [317][318][319][320][321][322][323][324] 2001.

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“…The most commonly used in vivo assays are the corneal micropocket assay, the chorioallantoic membrane (CAM) assay, and the dorsal skin-fold assay (Fournier et al, 1981;Jakob et al, 1978;Kenyon et al, 1996). To study the efficacy of angiogenic compounds, the corneal micropocket assay, which consists of an avascular environment, is often used.…”

mentioning

confidence: 99%

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Deckers

Pluijm

Dooijewaard

et al. 2001

Laboratory Investigation

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SUMMARY:Fetal mouse metatarsals are well-known models to study cartilage differentiation and osteoclastic resorption. We show here the outgrowth of PECAM-1 positive tubelike structures from the bone rudiments. This feature can be used to study angiogenesis in vitro. The area of outgrowth significantly increased with culture time, as shown by computerized image analysis of PECAM-1 positive tubelike structures. Treatment with recombinant human vascular endothelial growth factor (rhVEGF-A) stimulated the formation of tubelike structures. Treatment of explants with the angiogenesis inhibitor endostatin, the chemokine IP-10, and the thalidomide derivative phatolyl glutamic acid (PG-acid) resulted in an inhibition of the formation of PECAM-1 positive tubelike structures of 48.8% (Ϯ 4%), 50.2% (Ϯ12%), and 80.8% (Ϯ3%), respectively. Outgrowth of tubelike structures was partly dependent on endogenous VEGF-A because treatment with anti-mVEGF-A and truncated VEGF receptor 1 (soluble fms-like tyrosine kinase 1, sFlt1) strongly inhibited the formation of tubelike structures 74% (Ϯ 4%) and 38% (Ϯ 5%), respectively. Neither onset of tube formation nor total area of tubelike structures were changed when metatarsals were cultured on a fibrin gel or collagen type I gel. Tube formation required activation of matrix metalloproteinases because treatment of the bones with an inhibitor of matrix metalloproteinases completely inhibited migration and tube formation, whereas treatment with an inhibitor of plasmin had no effect. In conclusion, we describe a new in vitro model to study angiogenesis that can be used to test the angiogenic or antiangiogenic potential of novel test compounds that also combines the multicellularity of in vivo assays with the accessibility and flexibility of in vitro assays. (Lab Invest 2001, 81:5-15).

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The chick embryo chorioallantoic membrane as a bioassay for angiogenesis factors: Reactions induced by carrier materials

Cited by 48 publications

References 7 publications

Angiogenesis and Fibroblast Growth Factors (FGFs) in a Three-Dimensional Collagen Gel Culture

Angiogenesis and Fibroblast Growth Factors (FGFs) in a Three-Dimensional Collagen Gel Culture

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

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