The Chromatin Remodeling Factor BrCHR39 Targets DNA Methylation to Positively Regulate Apical Dominance in Brassica rapa

Zhu, Wei; Xie, Zhengqing; Chu, Zhenni; Ding, Yakun; Shi, Guiyang; Chen, Weiwei; Wei, Xiaochun; Yuan, Yuxiang; Wei, Fang; Tian, Bin

doi:10.3390/plants12061384

Cited by 1 publication

(1 citation statement)

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“…There are many potential mechanisms causing transcriptome shock, such as transposable elements [71], alternative splicing [72], and epigenetic modification [73,74], which have yet to be fully elucidated so far. Cis-and trans-regulation are among the most critical causes of transcriptome shock, and transcription factors significantly influence the generation of transcriptome shock [18].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

Zhou,

Zhang,

et al. 2023

IJMS

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Interspecific crosses that fuse the genomes of two different species may result in overall gene expression changes in the hybrid progeny, called ‘transcriptome shock’. To better understand the expression pattern after genome merging during the early stages of allopolyploid formation, we performed RNA sequencing analysis on developing embryos of Brassica rapa, B. napus, and their synthesized allotriploid hybrids. Here, we show that the transcriptome shock occurs in the developing seeds of the hybrids. Of the homoeologous gene pairs, 17.1% exhibit expression bias, with an overall expression bias toward B. rapa. The expression level dominance also biases toward B. rapa, mainly induced by the expression change in homoeologous genes from B. napus. Functional enrichment analysis revealed significant differences in differentially expressed genes (DEGs) related to photosynthesis, hormone synthesis, and other pathways. Further study showed that significant changes in the expression levels of the key transcription factors (TFs) could regulate the overall interaction network in the developing embryo, which might be an essential cause of phenotype change. In conclusion, the present results have revealed the global changes in gene expression patterns in developing seeds of the hybrid between B. rapa and B. napus, and provided novel insights into the occurrence of transcriptome shock for harnessing heterosis.

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