The clinical and prognostic value of CXCL8 in cervical carcinoma patients: immunohistochemical analysis

Yan, Ruiling; Shuai, Han-Lin; Luo, Xin; Wang, Xueqin; Guan, Baozhang

doi:10.1042/bsr20171021

Cited by 31 publications

(23 citation statements)

References 36 publications

(54 reference statements)

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“…Quercetin reduces the expression of CXCL8, IL2, IL8 and IL6. CXCL8, a proinflammatory oncogene is highly expressed in cervical cancer tissues [83]. IL-2, IL-8 and IL-6 expression is significantly correlated with poor prognosis [84–86].…”

Section: Discussionmentioning

confidence: 99%

Quercetin modulates signaling pathways and induces apoptosis in cervical cancer cells

Sundaram¹,

Raina²,

Afroze³

et al. 2019

Bioscience Reports

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Cancer cells have the unique ability to overcome natural defense mechanisms, undergo unchecked proliferation and evade apoptosis. While chemotherapeutic drugs address this, they are plagued by a long list of side effects and have a poor success rate. This has spurred researchers to identify safer bioactive compounds that possess chemopreventive and therapeutic properties. A wide range of experimental as well as epidemiological data encourage the use of dietary agents to impede or delay different stages of cancer. In the present study, we have examined the anti-ancer property of ubiquitous phytochemical quercetin by using cell viability assay, flow cytometry, nuclear morphology, colony formation, scratch wound assay, DNA fragmentation and comet assay. Further, qPCR analysis of various genes involved in apoptosis, cell cycle regulation, metastasis and different signal transduction pathways was performed. Proteome profiler was used to quantitate the expression of several of these proteins. We find that quercetin decreases cell viability, reduces colony formation, promotes G2-M cell cycle arrest, induces DNA damage and encourages apoptosis. Quercetin induces apoptosis via activating both apoptotic pathways with a stronger effect of the extrinsic pathway relying on the combined power of TRAIL, FASL and TNF with up-regulation of caspases and pro-apoptotic genes. Quercetin could inhibit anti-apoptotic proteins by docking studies. Further, quercetin blocks PI3K, MAPK and WNT pathways. Anticancer effect of quercetin observed in cell-based assays were corroborated by molecular biology studies and yielded valuable mechanistic information. Quercetin appears to be a promising candidate with chemopreventive and chemotherapeutic potential and warrants further research.

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Section: Discussionmentioning

confidence: 99%

Quercetin modulates signaling pathways and induces apoptosis in cervical cancer cells

Sundaram¹,

Raina²,

Afroze³

et al. 2019

Bioscience Reports

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“…CXCL8 also promotes angiogenesis and cell migration, induces EMT in CRC [183,186], attracts and activates MDSCs to form NETs, and helps tumors evade the immune system [187]. Due to the above protumor effects, high expression of CXCL8 potentially serves as an unfavorable prognostic marker in numerous human cancers, including CRC [167,188], cervical cancer [189], and lung adenocarcinoma [190]. Consistently, CXCL8 has also been a strong predictor for poor outcome in various treatments including chemo-immunotherapy [191] in pancreatic cancer or aflibercept therapy in metastatic CRC [192].…”

Section: Cxcl8mentioning

confidence: 99%

Chemokines and their Receptors: Multifaceted Roles in Cancer Progression and Potential Value as Cancer Prognostic Markers

Thu

Lee

Cho

2020

Cancers

167

131

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Chemokines are chemotactic cytokines that mediate immune cell chemotaxis and lymphoid tissue development. Recent advances have indicated that chemokines and their cognate receptors play critical roles in cancer-related inflammation and cancer progression. On the basis of these findings, the chemokine system has become a new potential drug target for cancer immunotherapy. In this review, we summarize the essential roles of the complex network of chemokines and their receptors in cancer progression. Furthermore, we discuss the potential value of the chemokine system as a cancer prognostic marker. The chemokine system regulates the infiltration of immune cells into the tumor microenvironment, which induces both pro- and anti-immunity and promotes or suppresses tumor growth and proliferation, angiogenesis, and metastasis. Increasing evidence indicates the promising prognostic value of the chemokine system in cancer patients. While CCL2, CXCL10, and CX3CL1/CX3CR1 can serve as favorable or unfavorable prognostic factors depending on the cancer types, CCL14 and XCL1 possess good prognostic value. Other chemokines such as CXCL1, CXCL8, and CXCL12 are poor prognostic markers. Despite vast advances in our understanding of the complex nature of the chemokine system in tumor biology, knowledge about the multifaceted roles of the chemokine system in different types of cancers is still limited. Further studies are necessary to decipher distinct roles within the chemokine system in terms of cancer progression and to validate their potential value in cancer prognosis.

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“…Interestingly, all of the above genes have been shown to be associated with cervical cancer. Yan R et al found that CXCL8 had prognostic value in cervical carcinoma patients [32]. Tian R et al identi ed the role of MMP1 in cervical cancer [33].…”

Section: Similar To Our Ndingsstudies Have Reported That Microtubulementioning

confidence: 99%

Identification of Two Novel Genes SNX10 and PTGDS with Cervical Squamous Cell Carcinoma Prognosis

Jiang¹,

Cao²,

Gao³

et al. 2020

Preprint

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Background: Cervical cancer (CC) is the primary cause of death in women. This study sought to investigate the therapeutic targets of CC. Methods: We downloaded four gene expression profiles from GEO. The RRA method was used to integrate and screen DEGs between CC and normal samples. Functional analysis was performed by clusterprofiler. We built PPI network by STRING and selected hub modules via MCODE. CMap was used to find molecules with therapeutic potential for CC. We also validated hub genes in GEO datasets, GEPIA, immunohistochemistry. Cox regression analysis, TCGA methylation analysis and ONCOMINE were carried out. ROC curve analysis and GSEA were also done to dig out the significance of hub genes. Results: Functional analysis revealed that DEGs were significantly enriched in binding, cell proliferation, transcriptional activity and cell cycle regulation. PPI network screened 30 prominent proteins, with CDK1 having the strongest association with CC. Cmap showed that apigenin, thioguanine and trichostatin A might be used to treat CC. Eight genes were screened out through GEPIA. Of them, only PTGDS and SNX10 have not been reported in CC related articles. The validation in GEO showed that PTGDS showed low expression in tumor tissues while SNX10 showed high expression in tumor tissues. Their expression profiles were consistent with the results in immunohistochemistry. They can distinguish CC and normal tissue and have good diagnostic efficiency. GSEA showed that the two genes were associated with the chemokine signaling pathway. TCGA methylation analysis showed that patients with low-expressed and hyper-methylated PTGDS had a bad prognosis than the patients with high-expressed and hypo-methylated PTGDS. Cox regression analysis showed that SNX10 and PTGDS were independent prognostic indicators for OS among CC patients. Conclusions: In conclusion, PTGDS and SNX10 showed abnormal expression and methylation in CC. Both genes could be used to develop new target treatments for CC.

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The clinical and prognostic value of CXCL8 in cervical carcinoma patients: immunohistochemical analysis

Cited by 31 publications

References 36 publications

Quercetin modulates signaling pathways and induces apoptosis in cervical cancer cells

Quercetin modulates signaling pathways and induces apoptosis in cervical cancer cells

Chemokines and their Receptors: Multifaceted Roles in Cancer Progression and Potential Value as Cancer Prognostic Markers

Identification of Two Novel Genes SNX10 and PTGDS with Cervical Squamous Cell Carcinoma Prognosis

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