The clinical presentation and management of a naturally occurring Bluetongue virus infection in a pregnant Rottweiler dog

Hanekom, Josef; Hoepner, Sara Nicolene; Preez, Kelly du; Leisewitz, Andrew L.

doi:10.36303/jsava.509

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Bluetongue virus is also typically transmitted by culicoid midges to diverse vertebrates (primarily ruminants) around the world [92,96]. Like African horse sickness virus, it can also infect dogs and cause clinical disease through vector-borne transmission, possibly through ingestion of infected meat, or through contaminated vaccines [97][98][99][100][101][102], and it also appears to infect wild carnivores widely [103]. Importantly, several other lesser-studied orbiviruses are zoonotic (e.g.…”

Section: Discussionmentioning

confidence: 99%

Viruses of free-roaming and hunting dogs in Uganda show elevated prevalence, richness and abundance across a gradient of contact with wildlife

Tayebwa,

Hyeroba,

Dunn

et al. 2024

Journal of General Virology

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Domestic dogs (Canis lupus familiaris) live with humans, frequently contact other animals and may serve as intermediary hosts for the transmission of viruses. Free-roaming dogs, which account for over 70% of the world’s domestic dog population, may pose a particularly high risk in this regard. We conducted an epidemiological study of dog viromes in three locations in Uganda, representing low, medium and high rates of contact with wildlife, ranging from dogs owned specifically for traditional hunting in a biodiversity and disease ‘hotspot’ to pets in an affluent suburb. We quantified rates of contact between dogs and wildlife through owner interviews and conducted canine veterinary health assessments. We then applied broad-spectrum viral metagenomics to blood plasma samples, from which we identified 46 viruses, 44 of which were previously undescribed, in three viral families, Sedoreoviridae, Parvoviridae and Anelloviridae. All 46 viruses (100 %) occurred in the high-contact population of dogs compared to 63 % and 39 % in the medium- and low-contact populations, respectively. Viral prevalence ranged from 2.1 % to 92.0 % among viruses and was highest, on average, in the high-contact population (22.3 %), followed by the medium-contact (12.3 %) and low-contact (4.8 %) populations. Viral richness (number of viruses per dog) ranged from 0 to 27 and was markedly higher, on average, in the high-contact population (10.2) than in the medium-contact (5.7) or low-contact (2.3) populations. Viral richness was strongly positively correlated with the number of times per year that a dog was fed wildlife and negatively correlated with the body condition score, body temperature and packed cell volume. Viral abundance (cumulative normalized metagenomic read density) varied 124-fold among dogs and was, on average, 4.1-fold higher and 2.4-fold higher in the high-contact population of dogs than in the low-contact or medium-contact populations, respectively. Viral abundance was also strongly positively correlated with the number of times per year that a dog was fed wildlife, negatively correlated with packed cell volume and positively correlated with white blood cell count. These trends were driven by nine viruses in the family Anelloviridae, genus Thetatorquevirus, and by one novel virus in the family Sedoreoviridae, genus Orbivirus. The genus Orbivirus contains zoonotic viruses and viruses that dogs can acquire through ingestion of infected meat. Overall, our findings show that viral prevalence, richness and abundance increased across a gradient of contact between dogs and wildlife and that the health status of the dog modified viral infection. Other ecological, geographic and social factors may also have contributed to these trends. Our finding of a novel orbivirus in dogs with high wildlife contact supports the idea that free-roaming dogs may serve as intermediary hosts for viruses of medical importance to humans and other animals.

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Section: Discussionmentioning

confidence: 99%

Viruses of free-roaming and hunting dogs in Uganda show elevated prevalence, richness and abundance across a gradient of contact with wildlife

Tayebwa,

Hyeroba,

Dunn

et al. 2024

Journal of General Virology

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Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco

Zahri,

Ahlamine,

Abou-Elaaz

et al. 2024

Parasite

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Blood-feeding arthropods are involved in the transmission of several pathogens that have a major impact on public health. Entomological investigations highlighted the composition, abundance, and diversity of flying hematophagous arthropods at four dog shelters located in central Morocco during an eight-month study, with the aim of discussing their vectorial roles and assessing the risk of these shelters as foci for zoonotic diseases. Monitoring of the arthropod fauna for 64 catch nights resulted in the collection of 2,321 biting midges (Ceratopogonidae), 570 mosquitoes (Culicidae), and 475 sand flies (Psychodidae). Fourteen Culicoides species were recorded and dominant species were Culicoides imicola (55.96%), C. paolae (16.07%), C. circumscriptus (10.29%), and C. newsteadi (5.77%). Three mosquito species were collected, including Culex pipiens s.l. (96.84%), Culiseta longiareolata (2.80%), and Cx. perexiguus (0.36%). Ten sand fly species were collected, including seven Phlebotomus species (62.70%) and three Sergentomyia species (37.30%); Sergentomyia minuta was the most dominant species (34.31%), followed by Phlebotomus sergenti (32.42%), typical Ph. perniciosus (8.63%), Ph. alexandri (6.94%), and Ph. riouxi (6.52%). The coexistence of several vectors in these study areas indicates the potential circulation of a wide range of pathogens, including zoonotic ones, thus requiring the implementation of surveillance and control programs to prevent the emergence and spread of disease outbreaks.

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Bluetongue Virus Infection in Farm Dogs Exposed to an Infected Sheep Flock in South Africa

Hanekom,

Ebersohn,

Penzhorn

et al. 2024

Transboundary and Emerging Diseases

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In 2021, a pregnant Rottweiler dog living on a sheep farm was diagnosed with clinical bluetongue (BT) infection. This study reports on the investigation of this farm where bluetongue virus (BTV) infection was diagnosed in this atypical host species. Samples were collected during farm visits 14, 28, 60, and 89 days after the onset of clinical signs in the pregnant Rottweiler. Blood was collected from all farm dogs (n = 6) and tested for BTV genome using a reverse‐transcriptase quantitative PCR (RT‐qPCR) assay and BTV antibodies with the competitive ELISA (cELISA) and dogs positive by RT‐qPCR were further tested using virus neutralization (VN) serological testing. Blood was also collected from 16 sick sheep and tested using RT‐qPCR. Midges were trapped on the study farm using an Onderstepoort UV light trap placed above a sheep pen for 36 hr at the first farm (14 days) visit. Parous/gravid midges were tested by BTV RT‐qPCR in batches of up to 200 midges per species. Blood‐fed midges (n = 308) were tested using a PCR species probe (KAPA Multiplex Master Mix) to identify the host species on which the midge had fed. Three dogs (n = 3/6) had detectable BTV RNA with RT‐qPCR and high VN antibody titers to BTV. All RT‐qPCR‐positive dogs and one additional dog tested cELISA seropositive (n = 4/6). Bluetongue virus RNA was detected in 5/16 sheep tested. The most abundant midge species was Culicoides imicola (99.3%) and BTV was only detected in this species (n = 3/4 batches of 200 parous midges). Dog blood was not detected in any blood‐fed midges tested. The occurrence of natural BT viraemia in exposed dogs creates a potential risk of BTV entry into BT‐free countries through dog importation. It remains unclear whether BT viremia in dogs is capable of onward transmission.

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The clinical presentation and management of a naturally occurring Bluetongue virus infection in a pregnant Rottweiler dog

Cited by 3 publications

References 22 publications

Viruses of free-roaming and hunting dogs in Uganda show elevated prevalence, richness and abundance across a gradient of contact with wildlife

Viruses of free-roaming and hunting dogs in Uganda show elevated prevalence, richness and abundance across a gradient of contact with wildlife

Diversity of biting midges, mosquitoes and sand flies at four dog shelters in rural and peri-urban areas of Central Morocco

Bluetongue Virus Infection in Farm Dogs Exposed to an Infected Sheep Flock in South Africa

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