The Close Relationship between DNA Replication and the Selection of Differentiation Lineages of Human Erythroleukemia Cell Lines K562, HEL, and TF1 into Either Erythroid or Megakaryocytic Lineages

Murate, Takashi; Saga, Shinsuke; Hotta, Tomomitsu; Asano, Haruhiko; Ito, Tomoki; Kato, Kohji; Tsushita, Keitaro; Kinoshita, Takafumi; Ichikawa, Akihiko; Yoshida, Shonen

doi:10.1006/excr.1993.1219

Cited by 43 publications

(38 citation statements)

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“…6 Megakaryocytic and erythroid differentiation has been shown to be a property of several established cell lines including K562, HEL and MEG-01 cells. [7][8][9][10] Differentiation of these cell lines can be modulated by treatment with PMA, hemin or butyric acid. 10 Megakaryocytic differentiation of K562 cells induced by PMA mimics, in part, the physiologic process that takes place in the bone marrow in response to a variety of stimuli.…”

Section: Introductionmentioning

confidence: 99%

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

Shelly¹,

Petruzzelli

Herrera³

1998

Leukemia

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Section: Introductionmentioning

confidence: 99%

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

Shelly¹,

Petruzzelli

Herrera³

1998

Leukemia

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“…MK-differentiating cells do not show the cytoplasmic von Willebrand factor expression or high nuclear lobation 27,28 (data not shown).…”

Section: Discussionmentioning

confidence: 88%

“…Notably, VEGF stimulation is sufficient per se to induce MK differentiation, indicating a key role for KDR in megakaryopoiesis. Accordingly, TF1 cells spontaneously secrete VEGF and express KDR, and can undergo moderate MK differentiation 22,27,28 (see also Figure 1, Tables 1-3). In particular, a moderate but significant proportion of untreated cells acquire the MK phenotype and show polylobated nuclei, indicating a spontaneous MK differentiation, possibly due to VEGF autocrine stimulation (Table 1).…”

Section: Discussionmentioning

confidence: 97%

“…TF1 cells differentiate into erythroid or MK lineage upon appropriate stimulation: TF1 cells are therefore suitable for the study of erythro-MK differentiation. 27,28 TF1 wt cells secrete significant amounts of VEGF in the culture medium (in a representative experiment, 170 pg/ml/10 5 cells of VEGF were detected in the cell culture supernatant) and express low levels of KDR protein and mRNA (Figure 1). Notably, TF1 wt cells do not express other VEGF receptors, such as Flt1 or Flt4, providing a useful tool to investigate the unique effects of KDR, independently of the other VEGF receptors (Figure 1a).…”

Section: Establishment Of Kdr-expressing Tf1 Cell Clones (Tf1-kdr)mentioning

confidence: 99%

“…Indeed, TF1 cells share the phenotype of normal HPCs (i.e., they express CD34 and c-kit) and are granulocyte macrophage-colonystimulating factor (GM-CSF) dependent. 27,28 TF1 cells are also suitable to study erythro-MK differentiation, as they can massively differentiate into erythroid or MK lineage upon appropriate stimulation: a low rate of spontaneous MK differentiation can also be observed. 27,28 In this study, we induced enforced expression of KDR in TF1 cell line to specifically study the effects of KDR stimulation on human early hematopoietic cells.…”

Section: Introductionmentioning

confidence: 99%

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Enforced expression of KDR receptor promotes proliferation, survival and megakaryocytic differentiation of TF1 progenitor cell line

et al. 2005

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A newly established megakaryoblastic/erythroid cell line that differentiates to red cells in the presence of erythropoietin and produces platelet‐like particles

et al. 1995

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In August, 1992, we established a leukemic cell line (NS-Meg) from a patient in megakaryoblastic transformation of Philadelphia chromosome-positive chronic myeloid leukemia. The NS-Meg cells were positive for alpha-naphthyl acetate esterase and periodic acid-Schiff (PAS) staining and for surface CD4, CD7, CD13, CD34, CD41a, and glycophorin A antigens. Ultrastructurally, the cells had alpha-granules, demarcation membranes, and platelet peroxidase activity. The NS-Meg cells spontaneously produced platelet-like particles which contained alpha-granules, mitochondria and dense bodies, strongly suggesting platelet production. Erythropoietin (Epo), granulocyte/macrophage colony stimulating factor(GM-CSF), and interleukin 3 (IL-3) promoted the growth of NS-Meg cells. Phorbol-12-myristate-13-acetate increased the expression of both CD41a and CD61 antigens. Ten-day exposure to Epo induced mature erythroblasts and red cells. These benzidine-positive cells were positive for hemoglobin F staining. Untreated NS-Meg cells expressed mRNA for the Epo receptor (EpoR), for GATA-1, and for alpha 1, alpha 2 and gamma globin genes. These results indicate that NS-Meg cells undergo terminal differentiation of both megakaryocytic and erythroid lineages. This cell line should be a very useful tool for the investigation of both megakaryocytic and erythroid maturation.

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The Close Relationship between DNA Replication and the Selection of Differentiation Lineages of Human Erythroleukemia Cell Lines K562, HEL, and TF1 into Either Erythroid or Megakaryocytic Lineages

Cited by 43 publications

References 0 publications

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

PMA-induced phenotypic changes in K562 cells: MAPK-dependent and -independent events

Enforced expression of KDR receptor promotes proliferation, survival and megakaryocytic differentiation of TF1 progenitor cell line

A newly established megakaryoblastic/erythroid cell line that differentiates to red cells in the presence of erythropoietin and produces platelet‐like particles

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