Proceedings of the 4th International Symposium on Current Progress in Mathematics and Sciences (Iscpms2018) 2019
DOI: 10.1063/1.5132521
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The combination effect 6 % of glycerol and skim milk on spermatozoa motility of giant grouper Epinephelus lanceolatus (Bloch 1970) after frozen

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“…Studi terkait penerapan protokol pada kriopreservasi sperma ikan lokal Indonesia telah dilaporkan pada beberapa spesies ikan air tawar, diantaranya gabus Channa striata (Mangkunegara et al, 2019), botia Chromobotia macracanthus , bader Barbonymus gonionotus (Abinawanto et al, 2013;Abinawanto et al, 2016;Abinawanto et al, 2018), baung Mystus nemurus (Muchlisin dan Siti-Azizah, 2009), gurami Osphronemus goramy (Abinawanto et al, 2011;Abinawanto et al, 2012a;Abinawanto et al, 2012b;Abinawanto et al, 2015;Abinawanto dan Putri, 2017;Abinawanto et al, 2017b), nilem Osteochilus hasseltii atau Osteochilus vittatus (Sunarma et al, 2007;Sunarma et al, 2008), kawan Poropontius tawarensis (Muthmainnah et al, 2019), depik Rasbora tawarensis (Muchlisin et al, 2020), keureling Tor soro (Zairin et al, 2005;Alifiani et al, 2020;Fatriani et al, 2020;Harjanti et al, 2020;Laeni et al, 2020;Pamungkas et al, 2020;Putri et al, 2020;Vardini et al, 2020;Wulandari et al, 2020), dan ikan air laut yaitu ikan kerapu, Ephinephelus lanceolatus (Afni et al, 2019;Pratiwi et al, 2019;Sudrajat et al, 2019;Widyaningsih et al, 2019).…”
Section: Extender Dan Krioprotektanunclassified
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“…Studi terkait penerapan protokol pada kriopreservasi sperma ikan lokal Indonesia telah dilaporkan pada beberapa spesies ikan air tawar, diantaranya gabus Channa striata (Mangkunegara et al, 2019), botia Chromobotia macracanthus , bader Barbonymus gonionotus (Abinawanto et al, 2013;Abinawanto et al, 2016;Abinawanto et al, 2018), baung Mystus nemurus (Muchlisin dan Siti-Azizah, 2009), gurami Osphronemus goramy (Abinawanto et al, 2011;Abinawanto et al, 2012a;Abinawanto et al, 2012b;Abinawanto et al, 2015;Abinawanto dan Putri, 2017;Abinawanto et al, 2017b), nilem Osteochilus hasseltii atau Osteochilus vittatus (Sunarma et al, 2007;Sunarma et al, 2008), kawan Poropontius tawarensis (Muthmainnah et al, 2019), depik Rasbora tawarensis (Muchlisin et al, 2020), keureling Tor soro (Zairin et al, 2005;Alifiani et al, 2020;Fatriani et al, 2020;Harjanti et al, 2020;Laeni et al, 2020;Pamungkas et al, 2020;Putri et al, 2020;Vardini et al, 2020;Wulandari et al, 2020), dan ikan air laut yaitu ikan kerapu, Ephinephelus lanceolatus (Afni et al, 2019;Pratiwi et al, 2019;Sudrajat et al, 2019;Widyaningsih et al, 2019).…”
Section: Extender Dan Krioprotektanunclassified
“…Penelitian kriopreservasi sperma E. lanceolatus telah dilaporkan oleh beberapa peneliti diantaranya, Afni et al (2019); Pratiwi et al (2019); Sudrajat et al (2019); Widyaningsih et al (2019). Bahan extender dan krioprotektan yang umum digunakan yaitu Marine Fish Ringer (MFR) (Afni et al, 2019;Pratiwi et al, 2019;Sudrajat et al, 2019;Widyaningsih et al, 2019) sebagai bahan extender dan gliserol 6% (Afni et al, 2019;Pratiwi et al, 2019;Sudrajat et al, 2019;Widyaningsih et al, 2019), susu soybean 5-25% (Afni et al, 2019), susu skim 5-25% (Sudrajat et al, 2019), kurma 5-25% (Widyaningsih et al, 2019), kuning telur 5-25% (Pratiwi et al, 2019) sebagai krioprotektan. Proses pembekuan sperma E. lanceolatus dilakukan pada suhu -20 ºC selama 48 jam dan di thawing pada suhu 45 ºC selama 0,5-10 menit.…”
Section: Kerapu Kertang (Ephinephelus Lanceolatus)unclassified