The Combination of B-Complex Vitamins and Ascorbic Acid in Aquedus Solutions**Research Department of Laborterapica S. A., Industrie Quimica e Farmactutica, Sao Paulo, Brazil

Gambier, Ana S.; Rahn, Erwin P.G.

doi:10.1002/jps.3030460216

Cited by 8 publications

(7 citation statements)

References 5 publications

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“…Important considerations that studies like this bring to light are the chain effects that can occur when multiple compounds are combined in a single set of conditions, aspects that are not necessarily clearly conveyed when looking at data from more simple mixtures. For example, thiamine/cyanocobalamin mixtures are stable at lower pH values, however, when riboflavin or nicotinamide are included, this degrades thiamine, whose degradation products now subsequently degrade cyanocobalamin (Gambier & Rahn, 1957). These chain reactions can be minimized by choice of component ratios in CCM.…”

Section: Discussionmentioning

confidence: 99%

“…Riboflavin is not usually affected by other CCM components but riboflavin will induce the degradation of many other CCM chloroflavin as the riboflavin degradation product (8) (Gambier & Rahn, 1957). This formation of chloroflavin from reaction with thiamine HCl is enhanced in the presence of ascorbic acid (Gambier & Rahn, 1957 (2012)); (b) key forms of riboflavin in electronic excitation and redox processes processes and products will depend on what types of ROS are generated/present in the system, that is, whether it involves type I or type II photooxidation. For further details on the photooxidative riboflavin-induced degradation processes and products of these particular AAs, we recommend the following resources: methionine (Tzeng, Lee, Chung, & DeVay, 1990;Yang, Ku, & Pratt, 1967);…”

Section: Interaction With Other Ccm Componentsmentioning

confidence: 99%

“…The most relevant interactions for CCM are the enhanced degradation by sulfites to pyrimidylmethane sulfonic acid 47 and thiazole 36 (Dwivedi & Arnold, 1973), oxidation by riboflavin to thiochrome (31) (Gambier & Rahn, 1957), and there is some suggestion of enhanced degradation through the reductive activity of ascorbic acid (Gambier & Rahn, 1957). Sulfur-based oxidants, such as cystine, enhance the degradation of thiamine, whereas some sulfur-based reductants, such as cysteine or other thiols, stabilize thiamine against degradation (Uprety, R., & Rao, 1961;Windheuser & Higuchi, 1963).…”

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confidence: 99%

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Vitamins in cell culture media: Stability and stabilization strategies

Schnellbaecher

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Bellmaine

et al. 2019

Biotech & Bioengineering

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Nowadays, chemically defined cell culture media (CCM) have replaced serum‐ and hydrolysate‐based media that rely on complex ingredients, such as yeast extracts or peptones. Benefits include a significantly lower lot‐to‐lot variability, more efficient manufacturing by reduction to essential components, and the ability to exclude components that may negatively influence growth, viability, or productivity. Even though current chemically defined CCMs provide an excellent basis for various mammalian biotechnological processes, vitamin instabilities are known to be a key factor contributing to the variabilities still present in liquid CCM as well as to short storage times. In this review, the chemical degradation pathways and products for the most relevant vitamins for CCM will be discussed, with a focus on the effects of light, oxygen, heat, and other CCM compounds. Different approaches to stabilize vitamins in solution, such as replacement with analogs, encapsulation, or the addition of stabilizing compounds will also be reviewed. While these vitamins and vitamin stabilization approaches are presented here as particular for CCM, the application of these concepts can also be considered relevant for pharmaceutical, medical, and food supplement purposes. More precise knowledge regarding vitamin instabilities will contribute to stabilize future formulations and thus decrease residual lot‐to‐lot variability.

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Section: Discussionmentioning

confidence: 99%

Section: Interaction With Other Ccm Componentsmentioning

confidence: 99%

mentioning

confidence: 99%

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Vitamins in cell culture media: Stability and stabilization strategies

Schnellbaecher

Binder

Bellmaine

et al. 2019

Biotech & Bioengineering

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“…All of these were U.S.P.-or N.F.-grade chemicals with the exception of the calcium sulfate which was in the form of CaS04.2Hz0. 4 The lubricant in all of the preparations was magnesium stearate, U.S.P.…”

Section: Study Of the Stability Of Multivitamin Chewablementioning

confidence: 99%

“…substrates with a cardiac glycoside or malonate it has been demonstrated that both may act through the removal of a rate-limiting step in the conversion of glycogen or glucose to pyruvate and thus, at least in part, affect energy production or utilization (4,5). Such an hypothesis does not account for the inhibition observed with high concentrations of malonate, but neither does it preclude the possibility of other concentration-related mechanisms.…”

mentioning

confidence: 99%