“…However, many ASs, including saccharin and aspartame, the so-called first generation sweeteners, have safety issues (Olney et al, 1996;Reuber, 1978;Van den Eeden et al, 1994). Although new generation sweeteners, such as acesulfame K (Mukherjee and Chakrabarti, 1997) and sucralose (Sasaki et al, 2002), have recently been approved, the potential risks to human health have not been firmly established. Over the past 3 decades, the potential hazard of carcinogenicity has been raised, but remains controversial (Weihrauch and Diehl, 2004).…”