Purpose: Muscle architecture is associated with motor performance and muscle injury. While muscle architecture and knee-flexor eccentric strength change with growth, the influence of anthropometric measures on these properties is rarely considered. This study aimed to investigate the relationship between hamstring muscle architecture and knee-flexor eccentric strength with anthropometric measurements. Methods: Sixty male footballers (16.6 [1.05] y) from the U16, U17, and U19 teams of an elite soccer club were included in this study. Fascicle length, pennation angle, and muscle thickness of the biceps femoris long head (BFlh) and semimembranosus muscles were measured in both legs using ultrasound. Knee-flexor eccentric strength, height, body mass, leg length, femur length, and peak height velocity (PHV) were measured within 1 week of the ultrasound images. A stepwise regression and 1-way analysis of variance tests were used to evaluate the effects of age, maturity, and anthropometric measurements on muscle properties. Results: Variance within BFlh and semimembranosus muscle thickness (r < .61), semimembranosus pennation angle (r < .58), and knee-flexor eccentric strength (r = .50) were highly related to body mass. We observed no significant correlations between muscle architecture and age (P > .29). However, moderately greater BFlh muscle thickness was shown for the post-PHV compared with the PHV group (effect size ± 90% CI: 0.72 ± 0.49). Conclusions: In conclusion, weak correlations between muscle architecture and anthropometric measurements suggest that other factors (ie, genetics, training regimen) influence muscle architecture. The moderate effect of maturity on BFlh muscle thickness strongly suggests post-PHV hypertrophy of the BFlh muscle. Our results confirmed previous findings that eccentric knee-flexor strength is influenced by body mass.