The Comparative Accuracy of 8 Commercial Rapid Immunochromatographic Assays for the Diagnosis of Acute Dengue Virus Infection

Abstract

Users of RDTs for dengue should be aware that many of these tests have a diagnostic accuracy that falls well below the manufacturers' claims. If an acute specimen yields a negative result, a convalescent serum sample should be tested to confirm the result. No RDT adequately differentiated primary and secondary dengue infections, and the tests should not be used for this purpose.

Multicountry Prospective Clinical Evaluation of Two Enzyme-Linked Immunosorbent Assays and Two Rapid Diagnostic Tests for Diagnosing Dengue Fever

“…Such differences may be related to site-specific interpretation of the manufacturer's instructions. A loss in sensitivity at an individual field site could also be reflective of serotype-or strain-specific differences at that particular site, as not all serotypes were seen at all sites, and serotype-specific variations have been previously reported (46)(47)(48)55). This variability demonstrates the importance of prospective real-world trials of diagnostic devices, particularly RDTs which are interpreted using the naked eye, as different operators and/or clinic conditions can affect the perceived product performance.…”

Evaluation of Commercially Available Anti–Dengue Virus Immunoglobulin M Tests

“…Limitations of anti-DENV IgM tests include their inability to identify the infecting DENV type and potential antibody cross-reactivity with other fl aviviruses (11,12). However, cross-reactivity to related viruses did not appear to be a problem with these tests.…”

Dengue myocarditis in Singapore: two case reports

“…A recent dengue secondary infection cannot be excluded in both cases due to the presence of positive IgM and IgG serology collected in the acute phase (sixth day of illness) [9]. The identification of DENV-2 in the second case is consistent with DENV-2 as the most prevalent dengue serotype circulating in Singapore, responsible for around 70 % of cases [1].…”