The comparison between AgB-ELISA and a new method of Nano-ELISA for diagnosis of hydatidosis in sheep

Abstract

Hydatidosis is one of the most important zoonotic diseases, and it is transmitted via dogs to intermediate hosts such as humans and domestic animals including sheep and cattle. Epidemiological studies and genetic investigations indicate that the sheep strain is the most common species causing hydatid cysts in humans. The prevalence and incidence of this disease are increasing. According to surveys, economic losses due to this parasite in intermediate hosts are significant. In this survey, 25 serum samples were obtained from newborn lambs as negative control and obtained 25 serum samples from infected sheep to hydatidosis as the positive control. Antigen B isolated from hydatid cysts fluid was used for designing ELISA methods. Using Antigen B in ELISA design for hydatidosis diagnosis has attracted researchers in recent years. During this study, an Iranian native B antigen was used to design the specific detection of hydatidosis in sheep using a specific ELISA technique. The first method used the anti-Sheep conjugate (SIGMA, at 1:3000 dilution), and the second method used gold nanoparticles in combination with anti-sheep conjugate. According to the results, sensitivity and specificity in sheep of the AgB-ELISA method were both 92% and for the Nano-ELISA with Gold Nanoparticles 100% and 96%, respectively. Moreover, results indicated that using Antigen B in ELISA design is valuable but specificity and sensitivity will increase significantly, especially in lower titer, when gold nanoparticles with anti-sheep conjugate are used.

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“…Both methods were highly sensitive (92% and 100%) and specific (96% and 96%) for sheep. Antigen B ELISAs with gold nanoparticles improved specificity and sensitivity, especially when gold nanoparticles were incorporated into the design [ 51 ].…”

“…Several surveys indicate that this parasite causes significant economic losses to all intermediate hosts [ 87 ]. In a study by Shirazi et al (2022) [ 51 ], serum samples were collected from newborns as negative controls and sheep with CE as positive controls. A specific ELISA technique was used to detect CE in sheep using an Iranian native B antigen.…”

“…In comparison, Nano-ELISAs with gold nanoparticles were 100% sensitive and 96% specific. When gold nanoparticles are conjugated with anti-sheep antibodies in an ELISA design, specificity and sensitivity will increase significantly, especially at low titers [ 51 ].…”

“…In comparison, Nano-ELISAs with gold nanoparticles were 100% sensitive and 96% specific. When gold nanoparticles are conjugated with anti-sheep antibodies in an ELISA design, specificity and sensitivity will increase significantly, especially at low titers [51]. Nanoparticles, such as silver or gold nanoparticles, have been integrated into the enzymelinked immunosorbent assay (ELISA) to boost its sensitivity and detection capabilities.…”