The Comparison of Heavy Metal Binding Properties and Tolerance between Transgenic Strains and Wild Type in Chlamydomonas reinhardtii

Chlamydomonas reinhardtii has many advantages compared with traditional systems for the molecular farming of recombinant proteins. These include low production costs, rapid scalability at pilot level, absence of human pathogens and the ability to fold and assemble complex proteins accurately. Currently, the successful expression of several proteins with pharmaceutical relevance has been reported from the nuclear and the chloroplastic genome of this alga, demonstrating its usefulness for biotechnological applications. However, several factors affect the level of recombinant protein expression in Chlamydomonas such as enhancer elements, codon dependency, sensitivity to proteases and transformation-associated genotypic modification. The present review outlines a number of strategies to increase protein yields and summarizes recent achievements in algal protein production including biopharmaceuticals such as vaccines, antibodies, hormones and enzymes with implications on health-related approaches. The current status of bioreactor developments for algal culture and the challenges of scale-up and optimization processes are also discussed.

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Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

Rosales‐Mendoza

2011

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Expression and function analysis of the metallothionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

Han

Lei

2008

SCI CHINA SER C

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The cDNA of the metallothionein-like (MT-like) gene from Festuca rubra cv. Merlin was optimized with bias codon of Chlamydomonas reinhardtii chloroplast genome. The optimized MT-like gene was delivered into C. reinhardtii chloroplast and the transgenic strains expressing MT-like gene was obtained. PCR-Southern blot and RT-PCR-Southern blot analysis demonstrated that the MT-like gene was integrated into chloroplast genome of C. reinhardtii and expressed at the transcriptional level. The cadmium binding capacity of the transgenic C. reinhardtii was determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) and the binding properties were analyzed. Results showed that the transgenic C. reinhardtii expressing the MT-like gene exhibited remarkably higher Cd(2+) binding capacity and grew to higher densities at toxic Cd(2+) concentrations (40-100 micromol/L) than the wild type strain, and that the IC(50) of Cd(2+) (3-d treating) to algal cell growth of transgenic strain was 55.43% higher than that of the wild type strain, indicating that the Cd(2+) binding capacity and Cd(2+) tolerance of C. reinhardtii was enhanced through the expression of the foreign MT-like gene in chloroplast.

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The Comparison of Heavy Metal Binding Properties and Tolerance between Transgenic Strains and Wild Type in Chlamydomonas reinhardtii

Cited by 2 publications

References 4 publications

Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

Expression and function analysis of the metallothionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast

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