The comparisons in protective mechanisms and efficiencies among dietary α-lipoic acid, β-glucan and l-carnitine on Nile tilapia infected by Aeromonas hydrophila

Lu, Dongliang; Limbu, Samwel Mchele; Lv, Hong‐Bo; Ma, Qiang; Chen, Liqiao; Zhang, Meiling; Du, Zhen‐Yu

doi:10.1016/j.fsi.2018.12.023

Cited by 56 publications

(26 citation statements)

References 39 publications

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“…In O. mossambicus, only 1% β-glucan in the diet significantly increased weight gain after an 8-week cultivation [42]. Similar results were also found in Dicentrachus labrax [43], O. nilotics [44], and Carassius auratus by adding β-glucan [45]. Therefore, the supplementary dose of β-glucan in the diet has a key influence on growth performance, especially in a stressful environment.…”

Section: Discussionsupporting

confidence: 68%

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Suo

Wang

et al. 2020

Animals

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Long-term exposure to hyperosmotic environments can induce severe immune damage and increase risk in tilapia breeding. As an effective immunoregulator, β-glucan has attracted extensive attention in nutritional research and given rise to high expectations of improving health status and alleviating organismal damage in tilapia, Oreochromis niloticus, in brackish water. In this study, an 8-week cultivation experiment was conducted on tilapia fed a basal diet or diets with β-glucan supplementation in freshwater (control) and brackish water. Growth performance, hematological aspects, immune cytokine expression, and the intestinal microbiota of tilapia were analyzed. The results indicated that supplementation with β-glucan significantly reduced the enlarged spleen of tilapia resulting from hypersaline stress. Tilapia fed β-glucan showed significantly-greater decreases in the red blood cell count, hematocrit, red cell distribution width, platelet count, and plateletcrit than those fed the basal diet. β-glucan significantly decreased the high expression of immune-related genes in the spleen induced by hyperosmotic stress. In the intestine, the high migration inhibitory factor-2 (MIF-2) and IL-1β gene expression induced by hypersaline stress was significantly reduced. β-glucan supplementation also significantly increased the abundance of beneficial microbiota such as Lactobacillus, Phycicoccus, and Rikenellaceae. Therefore, dietary β-glucan supplementation can significantly reduce spleen enlargement and improve immune function in tilapia in brackish water. β-glucan intake can also optimize the intestinal microbiota of tilapia in brackish water and improve fish health.

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Section: Discussionsupporting

confidence: 68%

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Suo

Wang

et al. 2020

Animals

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“…Probable reasons include the species-specific reaction and experimental circumstances (Dalmo & Bøgwald, 2008). This could be due to intensification in non-specific immune aspects and immune and antioxidant-related gene expression (Amphan, Unajak, Printrakoon, & Areechon, 2019;Lu et al, 2019). However, in the ATZ group, the values were significantly lower than those in the CNT group such reduction could be due to negatively impact of ATZ on fish yield, gill and resistance against infection, immunity and health status and cause oxidative damage (Toughan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…This indicates that supplementation with βG is valuable for Nile tilapia faced with a challenge. This may be due to the increase in non‐specific immune factors and immune and antioxidant‐related gene expression (Amphan et al, ; Lu et al, ). Feasible explanation of the decrease in immune‐toxicity of ATZ may be the promotion of anti‐oxidative components.…”

Section: Discussionmentioning

confidence: 99%

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

2020

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Three hundred Oreochromis niloticus were divided into five groups; the control fish (CNT) were fed a basal diet, beta glucan (βG) group was fed 0.5 g/kg−1 βG, atrazine (ATZ) group was exposed to 1/5 96‐hr LC50 (1.39 mg/L) ATZ, the (βG/ATZ) group was fed βG while being exposed to ATZ, and the (βG then ATZ) group was supplemented with βG for fifteen days before exposed to ATZ. ATZ exposure caused a decline in growth that was ameliorated by βG. ATZ reduced the levels of total and different types of leucocytes. Additionally, ATZ exposure caused reductions in total proteins, globulins, α 1‐globulin, α 2‐globulin, ɤ‐globulin, immunoglobulin M, lysozymes, superoxide dismutase, nitric oxide and catalase but increases in hepatic transaminases and malondialdehyde without any variations in albumin and β‐globulin. Exposure to ATZ also resulted in a rise in the mRNA level of IL‐8. In contrast, expression of IgM, SOD and CAT were decreased in the tilapias exposed to ATZ. Exposure to ATZ increases the susceptibility response to Aeromonas sobria challenge, as indicated by an increase in cumulative mortality post‐challenge. Supplementation with βG fifteen days before (βG then ATZ group), counteracted the adverse effects of ATZ on the immune, biochemical and antioxidants values, though only slight alleviation was observed with simultaneous treatment (βG/ATZ group). Our results established that ATZ has adverse impacts on immune responses, antioxidant equilibrium and its related genes. While, supplementation with βG before exposure to ATZ may be valuable for counteracting the possible damage caused by ATZ water pollution than its simultaneous treatment with ATZ.

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“…In order to evaluate the application of the compounds against bacteria, the antibacterial potential was tested in human pathogenic bacteria: S. aureus, E. faecalis, and E. coli, as well as in some bacteria species that cause disease in fish and humans (S. agalactiae and A. PLOS ONE hydrophila). The bacteria species S. agalacitiae and A. hydrophila are pathogenic for the fish Nile tilapia (Oreochromis niloticus), causing a foodborne disease in humans [81,82]. Often found on the skin and nasal fossae of healthy individuals S. aureus is a Gram-positive bacterium, considered an opportunistic microorganism and is one of the major causative agents of community and hospital infections worldwide [83] Several diseases are related to this bacterium, from superficial infections of the skin to more serious and deep infections, such as endocarditis, bacteremia, peritonitis, meningitis, necrotizing pneumonia and septic arthritis [84].…”

Section: Plos Onementioning

confidence: 99%

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

et al. 2020

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Parasitic diseases are a neglected and serious problem, especially in underdeveloped countries. Among the major parasitic diseases, Leishmaniasis figures as an urgent challenge due to its high incidence and severity. At the same time, the indiscriminate use of antibiotics by the population is increasing together with resistance to medicines. To address this problem, new antibiotic-like molecules that directly kill or inhibit the growth of microorganisms are necessary, where antimicrobial peptides (AMPs) can be of great help. In this work, the ferrocene molecule, one active compound with low levels of in vivo toxicity, was coupled to the N-terminus of the RP1 peptide (derived from the human chemokine CXCL4), aiming to evaluate how this change modifies the structure, biological activity, and toxicity of the peptide. The peptide and the conjugate were synthesized using the solid phase peptide synthesis (SPPS). Circular dichroism assays in PBS showed that the RP1 peptide and its conjugate had a typical spectrum for disordered structures. The Fc-RP1 presented antiamastigote activity against Leishmania amazonensis (IC 50 = 0.25 μmol L -1 ). In comparison with amphotericin B, a second-line drug approved for leishmaniasis treatment, (IC 50 = 0.63 μmol L -1 ), Fc-RP1 was more active and showed a 2.5-fold higher selectivity index. The RP1 peptide presented a MIC of 4.3 μmol L -1 against S. agalactiae, whilst Fc-RP1 was four times more active (MIC = 0.96 μmol L -1 ), indicating that ferrocene improved the antimicrobial activity against Gram-positive bacteria. The Fc-RP1 peptide also decreased the minimum inhibitory concentration (MIC) in the assays against E. faecalis (MIC = 7.9 μmol L -1 ), E. coli (MIC = 3.9 μmol L -1 ) and S. aureus (MIC = 3.9 μmol L -1 ). The cytotoxicity of the compounds was tested against HaCaT cells, and no significant activity at the highest concentration tested (500 μg. mL -1 ) was observed, showing the high potential of this new compound as a possible new drug. The coupling of ferrocene also increased the vesicle permeabilization of the peptide, showing a direct relation between high peptide concentration and high carboxyfluorescein release, which indicates the action mechanism by pore formation on the vesicles. Several studies have shown that ferrocene destabilizes cell membranes through lipid peroxidation, leading to cell lysis. It is noteworthy that the Fc-RP1 peptide synthesized here PLOS ONE

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The comparisons in protective mechanisms and efficiencies among dietary α-lipoic acid, β-glucan and l-carnitine on Nile tilapia infected by Aeromonas hydrophila

Cited by 56 publications

References 39 publications

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Alleviating effects of β‐glucan inOreochromis niloticuson growth performance, immune reactions, antioxidant, transcriptomics disorders and resistance toAeromonas sobriacaused by atrazine

Antimicrobial activity of RP-1 peptide conjugate with ferrocene group

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