The complete chloroplast genome sequence of Dendrobium thyrsiflorum (Orchidaceae) and its phylogenetic analysis

Abstract

Dendrobium thyrsiflorum Rchb.f., a native species to China, is widely used as an important garden flower and a traditional Chinese medicine. Herein, the complete chloroplast (cp) genome of D. thyrsiflorum was deciphered by high-throughput sequencing. The cp genome exhibited a typical quadripartite cycle of 151,686 bp in length, comprising of a pair of inverted repeats (IRa and IRb) of 26,293 bp which were intersected by a large single copy (LSC) region of 84,749 bp and a small single copy (SSC) region of 14,351 bp. A total of 126 genes were de novo assembled in this cp genome, including 78 protein genes, 40 tRNA genes and 8 rRNA genes. Among these genes, 86 genes (22 tRNAs and 64 coding genes) were single copy, the rest were two-copy genes, and the average of GC content of the whole genome is 37.55%. Phylogenetic trees showed that the D. thyrsiflorum was closely related to D. devonianum . This study provides molecular information for future evolution, genetic and molecular biology studies of Dendrobium .

Secondary Metabolomic Analysis and In Vitro Bioactivity Evaluation of Stems Provide a Comprehensive Comparison between Dendrobium chrysotoxum and Dendrobium thyrsiflorum

“…However, the DC stems are in short supply due to the scarcity of resources, and D. thyrsiflorum (DT) stems are consciously or unconsciously regarded as alternatives due to the high similarity in morphologies of species recorded in the Pharmacopoeia [ 6 ]. At present, the DT species is known as an endemic herb and important garden flower distributed in southeast China [ 7 ]. The phytochemical and bioactive information of DT stems is inadequate, hindering the development of the materials.…”

Effects of plant preservative mixture™ on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation

“…As a model, Dendrobium thyrsiflorum Rchb.f. is affected and threatened in its natural habitat due to its ornamental value and over-exploitation (Zhu et al 2019). We successfully applied these results in the culture establishment and conservation of other rare orchids, such as Dendrobium officinale and Nervilia spp.…”

The first complete chloroplast genome of Gentiana rigescens and its phylogenetic position in Gentianaceae

“…(Qi et al 2017). Due to the over-exploitation of wild resources and narrow distribution, the wild populations of G. rigescens are severely threatened (Zhu et al 2019). To date, the previous studies were mainly focused on its resource, chemical composition, genetic diversity, and pharmacodynamics (Xu et al 2009;Wu et al 2017), rarely involved in its genomes.…”