The complete chloroplast genome sequence of <i>Spiraea</i>×<i>vanhouttei</i> (Briot) Zabel (Rosaceae)

Chen, Min-Min; Wang, Ruihong; Sha, Hong-kun; Liu, Mingzhi; Tong, Jian-quan; He, Qiuling

doi:10.1080/23802359.2022.2052369

Cited by 1 publication

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“…To characterize the feature of among endemic species of Uncaria in China, five members of the Uncaria family were collected from Guangxi Province in China in July 2021 and subsequently deposited in the College of Life Sciences and Medicine, Zhejiang Sci-Tech University (Hangzhou, China), with herbarium numbers ZSTU70128 ( Uncaria rhynchophylla ), ZSTU09554 ( Uncaria macrophylla ), ZSTU01022 ( Uncaria hirsuta ), ZSTU09751 ( Uncaria sessilifructus ), and ZSTU70322 ( Uncaria scandens ). Following the manufacturer’s directions, DNA Plantzol Reagent (Invitrogen, Carlsbad, CA, USA) was used to extract the total DNA from the plant’s leaves [ 75 , 76 , 77 ]. Utilizing Illumina’s TruSeq Nano DNA Library Preparation kit (350 bp median insert), sequencing libraries were created in accordance with the manufacturer’s instructions [ 78 , 79 , 80 ].…”

Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Analysis of Chloroplast Genomes in Five Uncaria Species Endemic to China

Chen

Zhang

Liang

et al. 2022

IJMS

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Uncaria, a perennial vine from the Rubiaceae family, is a typical Chinese traditional medicine. Currently, uncertainty exists over the Uncaria genus’ evolutionary relationships and germplasm identification. The complete chloroplast genomes of four Uncaria species mentioned in the Chinese Pharmacopoeia and Uncaria scandens (an easily confused counterfeit) were sequenced and annotated. The findings demonstrated that the whole chloroplast genome of Uncaria genus is 153,780–155,138 bp in full length, encoding a total of 128–131 genes, containing 83–86 protein-coding genes, eight rRNAs and 37 tRNAs. These regions, which include eleven highly variable loci and 31–49 SSRs, can be used to create significant molecular markers for the Uncaria genus. The phylogenetic tree was constructed according to protein-coding genes and the whole chloroplast genome sequences of five Uncaria species using four methods. The topology of the two phylogenetic trees showed no difference. The sequences of U. rhynchophylla and U. scandens are clustered in one group, while the U. hirsuta and U. macrophylla are clustered in another group. U. sessilifructus is clustered together with the above two small clades. New insights on the relationship were revealed via phylogenetic research in five Uncaria species. This study will provide a theoretical basis for identifying U. rhynchophylla and its counterfeits, as well as the species of the Uncaria genus. This research provides the initial chloroplast genome report of Uncaria, contributes to elucidating the chloroplast genome evolution of Uncaria in China.

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