The complete DNA sequence of the mitochondrial genome of Sinonovacula constricta (Bivalvia: Solecurtidae)

Abstract

The nucleotide sequence of the mitochondrial genome of the solecurtidae Bivalvia mollusca Sinonovacula constricta (GenBank accession number EU880278) has been determined and is reported here. We determined the complete mitochondrial genome sequence using long-PCR and Shot Gun Sequencing. Contained within the 17 225 base pairs (bp) are the two ribosomal RNA genes and 12 protein coding genes typical of metazoan mitochondrial genomes. The S. constricta mitochondrial DNA (mtDNA) did not contain a gene for atp8, similar to the mtDNA of Crassostrea virginica, Crassostrea giga and Mytilus edulis. The S. constricta mtDNA is 67.0% A+T (A 25.9%, C 10.5%, G 22.5%, and T 41.1%). This value is higher than that for many invertebrate mitochondrial genomes. Only 19 putative tRNA genes are present in S. constricta and 27 noncoding regions, of which two are large in size. The trnE and trnW genes as well as a second trnS were absent in S. constricta. The gene arrangement of S. constricta is different from the other Bivalvia genomes.

The complete mitochondrial genome of the clamMactra veneriformis(Bivalvia: Mactridae): Has a unique non-coding region, missingatp8and typicaltRNASer

“…The complete mitochondrial (mt) genome of M. veneriformis is 16,854 bp in length and encodes 34 genes on the same strand, including 12 protein-coding genes (PCGs), 2 ribosomal RNA genes and 20 transfer RNA genes ( Figure 1A). The mt genome size is similar to that of Loripes lacteus (Dreyer and Steiner 2006), Sinonovacula constricta (Zheng et al 2010) and RZ Coelomactra antiquata (Meng et al 2012). Meanwhile, the mt genome size is shorter than that of other veneroids (Xu et al 2012) and within the size range of the bivalve mt genomes published to date (Smith and Snyder 2007).…”

Novel gene rearrangement in the mitochondrial genome of Siliqua minima (Bivalvia, Adapedonta) and phylogenetic implications for Imparidentia

“…constricta , of Pharidae, whose mitogenome was 17,225 bp in length, similar to that of S . minima [ 45 ]. S .…”

“…minima had 22 putative tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA), 12 PCGs and one control region (CR) including an origin of the light-strand replication (OL) region. According to our statistics, all species of Adapedonta we downloaded contained the atp8 gene, except for species of the family Pharidae [ 45 , 47 – 51 ]. The gene arrangement of the mitogenome of S .…”

The complete mitochondrial genome of Macrobrachium nipponense

“…The boundaries of the protein-coding genes (PCGs) and rRNA genes were inferred by comparisons with the amino acid or nucleotide sequences of other metazoans (Zheng et al, 2010). The 5′ and 3′ ends of the 16S rRNA and 12S rRNA genes were assumed to be adjacent to the flanking tRNA genes or protein genes.…”