The complete mitochondrial DNA sequence of the short mackerel (Rastrelliger brachysoma), and its phylogenetic position within Scombroidei, Perciformes

Abstract

In order to support studies of short mackerel population genetic structure in the Gulf of Thailand and phylogenetic relationships, the mitochondrial genome of the short mackerel, Rastrelliger brachysoma, has recently been determined by a partial cloning technique, long PCR with three pairs of newly designed primers and primer walking sequencing. The complete mitochondrial genome is 16,539 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) and a control region (CR) as in other bony fishes. Within the 845-bp CR, we identified several conserved motifs. The phylogeny obtained by Bayesian analyses based on two nucleotide datasets corresponding to the cytb and nd2 mitochondrial genes strongly support the inclusion of R. brachysoma within the monophyletic tribe of Scombrini in the family Scombridae. The obtained phylogeny also reveals high-statistical support for the existence of two distinct groups indicating that Scombroidei and Xiphioidei are two separate suborders.

The First Genome Survey and De Novo Assembly of the Short Mackerel (Rastrelliger brachysoma) and Indian Mackerel (Rastrelliger kanagurta)

“…However, the mtDNA sequence of the island mackerel ( Rastrelliger faughni ) was not yet complete at the time of the study and only DNA fragments from some genes (e.g., cytB , COX1 ) were present in the database. Moreover, R. brachysoma shared 99.9% similarities between this study and a previous study [ 34 ]. Only 10 single-nucleotide polymorphisms (SNPs) were found in the protein-coding genes.…”

Hydraulic control of tuna fins: A role for the lymphatic system in vertebrate locomotion

“…4A). Although the phylogeny of the Scombridae family tribes has been questioned (28), our finding corroborates a more recent whole–mitochondrial genome analysis (29) showing the proximity of tuna, bonito, and Spanish mackerel tribes (Fig. 4B).…”

Complete mitochondrial genome analysis of Japanese Spanish mackerel (Scomberomorus niphonius) with phylogenetic consideration

“…Species from family Scombridae, Nomeidae, Centrolophidaea, and Trichiuridae of Scombriformes were clustered into different sister groups (Figure 1). S. niphonius and the other two species from Scomberomorus (Scombridae) formed a cluster apart from the one comprising other genus from Scombridae in consistent with the study of Jondeung and Karinthanyakit (2010).…”