The complete mitochondrial genome analysis of the stonefly, Flavoperla sp. (Plecoptera: Perlidae)

Abstract

Flavoperla is a small genus in Acroneuriinae, which was established by Chu but was treated as a synonym for a long time until Uchida and Harper recognized Flavoperla as a valid genus. This genus includes 15 species in the world now, including six species from China. Here, we sequenced and annotated the mitochondrial genome of Flavoperla sp. under the next-generation sequence technology, the first representative of Genus Flavoperla. The complete mitochondrial genome of Flavoperla sp. is 15,796 bp in length with an A þ T content of 68.3% showed a positive AT-skew (0.037) and a negative GC-skew (À0.262). The gene order and organization of the mitochondrial genome is consistent with other stoneflies. The control region had the highest A þ T content. Eleven PCGs started with the typical codon (ATN), the remaining PCGs started with TTG (ND1and ND5). All PCGs terminated with TAA/TAG, except COII and ND5 used single T. The phylogenetic tree by maximum likelihood (ML) and Bayesian (BI) methods supported that Flavoperla sp. was the sister group to Niponiella limbatella.

Characterization of the complete mitochondrial genome of a stonefly, Perlesta teaysia (Plecoptera: Perlidae)

“…The genus Perlesta Banks, 1906 of the subfamily Acroneuriinae Klap alek, 1914 of Perlidae includes 33 species currently, of which only two are known from China (Wu 1938(Wu , 1948Mur anyi and Li 2016;DeWalt et al 2019). Although a few mitochondrial genomes of the Acroneuriinae have been previously published (Huang et al 2015;Cao, Wang, Zhang, et al 2019;Li et al 2019;Wang et al 2019) and listed in GenBank database, the mitochondrial genome of Perlesta is unavailable. Herein, we sequenced and annotated the complete mitochondrial genome sequence of the Nearctic P. teaysia Kirchner & Kondratieff, 1997 (GenBank accession number MN627432) by Illumina sequencing for the first time.…”

“… 2019 ; Wang et al. 2019 ) and listed in GenBank database, the mitochondrial genome of Perlesta is unavailable. Herein, we sequenced and annotated the complete mitochondrial genome sequence of the Nearctic P. teaysia Kirchner & Kondratieff, 1997 (GenBank accession number MN627432) by Illumina sequencing for the first time.…”