The complete mitochondrial genome sequence of Aspergillus flavus

Abstract

Aspergillus flavus is a haploid filamentous fungus that is common in the environment and has been implicated in human infections. The complete mitochondrial genome of A. flavus has been determined by high-throughput sequencing technology in this work. Our study revealed that the mitochondrial genome of A. flavus is 31,602 bp long, with an A + T content of 74.83%, which consists of a usual set of mitochondrial proteins and RNA genes, including large and small ribosomal RNAs, 15 proteins, and 20 tRNA genes and contains two introns. Notably, it also contains two hypothetical proteins without obvious homology to any known proteins. All structural genes are located on one strand and are apparently transcribed in one direction. Codon usage analysis indicated that all protein coding genes employ the standard fungal mitochondrial start and stop codons; and the nucleotide bias toward AT was also reflected in the codon usage. The complete mitochondrial genomes of A. flavus would be useful for future investigation of the genetic, evolution, and clinical identification of Aspergillus species.

Complete Mitochondrial Genome Sequences of Nine Aspergillus flavus Strains

“…All mitogenomes also contained ribosomal protein s5 and an intron-encoded LAGLIDADG endonuclease. Two mitochondrial rRNA genes encoding small and large ribosomal subunits and 27 tRNAs were identified in each mitogenome, as expected based on previously assembled Aspergillus mitogenome sequences ( 3 – 5 ).…”

“… Aspergillus flavus is a potent producer of aflatoxin B, a carcinogenic mycotoxin and a plant and opportunistic human pathogen ( 2 ). Despite the widespread availability of A. flavus genomic sequences, few assembled and annotated mitochondrial genome (mitogenome) sequences are available ( 3 – 5 ). Mitochondrial genes are linked to processes including metabolism, cell differentiation, and drug resistance ( 6 , 7 ).…”

Complete mitochondrial genome sequence of Aspergillus flavus SRRC1009: insight of intraspecific variations on A. flavus mitochondrial genomes

“…Aspergillus flavus produces aflatoxin, a notorious mycotoxin that can cause liver cancer (Squire 1981). To date many genome sequences of A. flavus were sequenced (Payne et al 2006;Nierman et al 2015;Weaver et al 2017); however, only three mitogenomes are available (Joardar et al 2012;Yan et al 2016;Park et al, under review). To understand intraspecific variations on A. flavus mitogenomes, we completed the mitogenome of A. flavus SRRC1009.…”

“… 2012 ; Yan et al. 2016 ; Park et al., under review ). To understand intraspecific variations on A. flavus mitogenomes, we completed the mitogenome of A. flavus SRRC1009.…”

“…The first mitogenome of A. flavus (Yan et al. 2016 ) presented different features in various aspects including length (31,602 bp) and GC ratio (25.2%). Based on our mitogenome, this might be another Aspergillus species together with the recent study (Park et al., under review ) and phylogenetic tree ( Figure 1(A) ).…”