The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus

Solís, Ignacio; García‐Arenal, Fernando

doi:10.1016/0042-6822(90)90520-2

Cited by 86 publications

(56 citation statements)

References 30 publications

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“…The genome of PMMV-S is 6357 nt long. It shares an overall sequence identity of 69.4% with the RNA of ToMV (Ohno et al, 1984), 68.5% with that of TMV (Goelet et al, 1982) and 64% with TMGMV (Solis & Garcia-Arenal, 1990), the other members of the tobamovirus group whose entire RNA sequences are already known. As shown in Table 1, also, PMMV-S shares a higher degree of amino acid sequence identity with ToMV than with TMV and TMGMV.…”

Section: Sequence Determination and Terminal Non-coding Regionsmentioning

confidence: 98%

“…( , 1982, Saito et aL (1988) and Meshi et al (1983), for ToMV, TMV, SHMV (30K and coat protein), CGMMV 30K and CGMMV-W CP, respectively. Data reported for two isolates of TMGMV (TMGMV a and TMGMV b) are from Solis & Garcia-Arenal (1990) and Nejidat et al (1991), respectively. t The sequences analysed correspond only to the readthrough part of the 183K protein.…”

Section: Organization Of the 126k/183k Gene Sequencementioning

confidence: 99%

“…Its putative translation product is 257 amino acids long with a calculated Mr of 28 347. Therefore, this is the first tobamovirus in which none of the reading frames overlap, since in ToMV, TMV, cucumber green mottle mosaic virus (CGMMV) and TMGMV the 5' end of the genes encoding the 30K protein overlap with the 3' end of those encoding the 183K proteins (Goelet et al, 1982;Ohno et al, 1984;Saito et al, 1988;Solis & Garcia-Arenal, 1990), and in sunn-hemp mosaic virus (SHMV) as well as in CGMMV (Meshi et al, 1982;Saito et al, 1988) their 30K-coding regions overlap at the 3' end with their coat protein genes.…”

Section: Organization Of the 30k Protein Genementioning

confidence: 99%

“…The complete nucleotide sequence of three other tobamoviruses, tobacco mosaic virus (TMV) (Goelet et al, 1982), tomato mosaic virus (ToMV) (Ohno et al, 1984) and tobacco mild green mottle virus (TMGMV) (Solis & Garcia-Arenal, 1990) have already been reported. The tobamoviral RNA encodes four different proteins: 126K, 183K, 30K and 17.5K, in that order.…”

Section: Introductionmentioning

confidence: 99%

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Nucleotide Sequence of the Genomic RNA of Pepper Mild Mottle Virus, a Resistance-breaking Tobamovirus in Pepper

Alonso

García-Luque

Cruz

et al. 1991

Journal of General Virology

104

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The entire genomic RNA of a Spanish isolate of pepper mild mottle virus (PMMV-S), a resistance-breaking virus in pepper, was cloned and sequenced and shown to be similar to other tobamoviruses in its genomic organization. It consisted of 6357 nucleotides (nt) and contained four open reading frames (ORFs) which encode a 126K protein and a readthrough 183K protein (nt 70 to 4908), a 28K protein (nt 4909 to 5682) and a 17.5K coat protein (nt 5685 to 6158). This is the first tobamovirus in which none of the ORFs overlap. Both its nucleic acid and predicted protein sequences were compared with the previously determined sequences of other tobamoviruses. The variations and similarities found and their relationship with the pathogenicity of this virus are discussed.

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Section: Sequence Determination and Terminal Non-coding Regionsmentioning

confidence: 98%

Section: Organization Of the 126k/183k Gene Sequencementioning

confidence: 99%

Section: Organization Of the 30k Protein Genementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nucleotide Sequence of the Genomic RNA of Pepper Mild Mottle Virus, a Resistance-breaking Tobamovirus in Pepper

Alonso

García-Luque

Cruz

et al. 1991

Journal of General Virology

104

View full text Add to dashboard Cite

show abstract

“…The closer sequence similarity of the STMV tRNAlike structure to that of TMV rather than to the helper TMGMV has been noted (Solis & Garcia-Arenal, 1990). The pseudoknot stalk region that is located upstream of the tRNA-like structure in STMV RNA (nucleotides 874 to 950) also shows more sequence and structural similarity with TMV RNA than with that of TMGMV.…”

mentioning

confidence: 99%

Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Gultyaev

Batenburg

Pleij

1994

Journal of General Virology

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Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications

Chan

Steinmetz

2022

ChemBioChem

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Tobacco mosaic virus (TMV) was the first virus to be discovered and it is now widely used as a tool for biological research and biotechnology applications. TMV particles can be decorated with functional molecules by genetic engineering or bioconjugation. However, this can destabilize the nanoparticles, and/or multiple rounds of modification may be necessary, reducing product yields and preventing the display of certain cargo molecules. To overcome these challenges, we used phage display technology and biopanning to isolate a TMV-binding peptide (TBP T25 ) with strong binding properties (IC 50 = 0.73 μM, K D = 0.16 μM), allowing the display of model cargos via a single mixing step. The TMV-binding peptide is specific for TMV but does not recognize free coat proteins and can therefore be used to decorate intact TMV or detect intact TMV particles in crude plant sap.

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The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus

Cited by 86 publications

References 30 publications

Nucleotide Sequence of the Genomic RNA of Pepper Mild Mottle Virus, a Resistance-breaking Tobamovirus in Pepper

Nucleotide Sequence of the Genomic RNA of Pepper Mild Mottle Virus, a Resistance-breaking Tobamovirus in Pepper

Similarities between the secondary structure of satellite tobacco mosaic virus and tobamovirus RNAs

Isolation of Tobacco Mosaic Virus‐Binding Peptides for Biotechnology Applications

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