The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA In higher eukaryotes

Abstract

We have determined the complete nucleotide sequence (4712 nucleotides) of the mouse 28S rRNA gene. Comparison with all other homologs indicates that the potential for major variations in size during the evolution has been restricted to a unique set of a few sites within a largely conserved secondary structure core. The D (divergent) domains, responsible for the large increase in size of the molecule from procaryotes to higher eukaryotes, represent half the mouse 28S rRNA length. They show a clear potential to form self-contained secondary structures. Their high GC content in vertebrates is correlated with the folding of very long stable stems. Their comparison with the two other vertebrates, xenopus and rat, reveals an history of repeated insertions and deletions. During the evolution of vertebrates, insertion or deletion of new sequence tracts preferentially takes place in the subareas of D domains where the more recently fixed insertions/deletions were located in the ancestor sequence. These D domains appear closely related to the transcribed spacers of rRNA precursor but a sizable fraction displays a much slower rate of sequence variation.

Reverse taxonomy: an approach towards determining the diversity of meiobenthic organisms based on ribosomal RNA signature sequences

“…Divergence profile of the sequences in our analysis correlated with secondary structure. The divergence measures are based on similarity values provided by the ARB program, the naming of the helices and loops follows Hassouna et al (1984). The depiction does not include indel differences, which can be substantial.…”

MIDA1 is a sequence specific DNA binding protein with novel DNA binding properties

“…Probes corresponding to the putative MIDA1 binding sites in the Hsp70.2 gene promoter [Hsp70.2(22 bp)] (Wisniewski et al 1990), the 28S rRNA gene [28S rRNA(22 bp)] (Hassouna et al 1984) and the core sequence of the selected clone #2 [#2(22 bp)], a member of the ( ) group as a positive control, were not bound by GST-DMIDA1 (Fig. 5B left).…”

A preliminary phylogenetic analysis of the Capsalidae (Platyhelminthes: Monogenea: Monopisthocotylea) inferred from large subunit rDNA sequences

“…Nucleotide data from the lsrDNA region of the 7 newly sequenced capsalid species produced an alignment of approximately 850 characters including domains C2 and D1 and partial domains C1 and D2 as defined by Hassouna, Michot & Bachellerie (1984).…”