The complete nucleotide sequence of the tobacco chloroplast genome

Shinozaki, Kazuo; Ohme, Masaru; Tanaka, Minoru; Wakasugi, Tatsuya; Hayshida, N.; Matsubayasha, T.; Zaita, N.; Chunwongse, Julapark; Obokata, Junichi; Yamaguchi‐Shinozaki, Kazuko; Ohto, Chikara; Torazawa, Keita; Meng, Bingkun; Sugita, Mamoru; Deno, Hiroshi; Kamogashira, Takashi; Yamada, Kyoji; Kusuda, Jun; Takaiwa, F.; Kata, Aleksandra; Tohdoh, Naoki; Shimada, Hiroaki; Sugiura, Masahiro

doi:10.1007/bf02669253

Cited by 147 publications

(96 citation statements)

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“…The comparison with those reveals that the N-terminal part is similar: the first amino acid residue (M) is cleaved off and the NH2-A-T(P)-X-T-sequence was found in all sources [6][7][8]18,19,26]. An SKXXP motive was also recognized as a conserved sequence at the N-terminus (fig.4).…”

Section: Discussionmentioning

confidence: 97%

“…The sequence of the 8 kDa protein from a eucaryotic alga, like C. reinhardii, provides information about structural variability of the psb H gene products, since so far only sequences of higher plants are available [7,8,18,19,26]. The comparison with those reveals that the N-terminal part is similar: the first amino acid residue (M) is cleaved off and the NH2-A-T(P)-X-T-sequence was found in all sources [6][7][8]18,19,26].…”

Section: Discussionmentioning

confidence: 99%

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N‐terminal sequence analysis of the 8 kDa protein inChlamydomonas reinhardiiLocalization of the phosphothreonine

Dedner

Meyer

Ashton³

et al. 1988

FEBS Letters

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A phosphorylated 8 kDa protein of Chlamydomonas reinhurdii thylakoids has been isolated and its N-terminal amino acid sequence determined by gas-phase sequencing. The sequence analysis of the 48 amino acid residues revealed that this protein is about 50% homologous to the psb H gene products of higher plants. In contrast to them, it contains an insert of seven amino acid residues (Ser-5 to Lys-1 1). The first threonine residue was phosphorylated as determined by 32P detection during sequencing and also by analysis of the modified degradation products in the chemical reaction of the Edman degradation process. This latter method allows the identification of phosphorylated threonine residues without radiolabelling the protein. (Chlamydomonus reinhardiz)

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

N‐terminal sequence analysis of the 8 kDa protein inChlamydomonas reinhardiiLocalization of the phosphothreonine

Dedner

Meyer

Ashton³

et al. 1988

FEBS Letters

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“…A preliminary sequence analysis of N. plumbaginifolia ptDNA in the region of the resistance mutations revealed a missing G at position 890 of the 16s rDNA, present in both the original SR1 mutant (Etzold et a/., 1987) and the wild-type (Shinozaki et a/., 1986b) of N. tabacum. Comparative sequence analysis of different ptDNA regions of N. tabacumand N. plumbaginifolia indicated that at least one nucleotide difference can be expected in a 200-300 bp region (Fejes eta/., 1990).…”

Section: Spectinomycin Resistance Mutations Can Appear In At Leastmentioning

confidence: 99%

Chloroplast transformation in plants: polyethylene glycol (PEG) treatment of protoplasts is an alternative to biolistic delivery systems

O’Neill¹,

Horváth²,

Horváth³

et al. 1993

Plant J

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SummaryNicotiana plumbaginifolia protoplasts were directly transformed by PEG treatment with a cloned 16s rRNA gene isolated from a double antibiotic-resistant Nicotiana tabacum plastid mutant. Putative plastid transformants were selected in cell culture by their spectinomycin resistance and identified by their unselected streptomycin resistance. Alternatively, cell lines were selected in the presence of both antibiotics. The cell line (and its regenerated plants) selected solely for spectinomycin resistance demonstrated an extensive segregation of streptomycin resistance in subsequent tests, while the double-selected line showed stable resistance for both antibiotics. The resistance markers were inherited maternally. In the putative plastid transformants the origin of the resistance mutations was identified by the absence of an Aatll site, missing in the donor N. tabacum plastid gene (spectinomycin resistance site) but present in that of wild-type N. plumbaginifolia, and a sequence analysis of the particular nucleotide changes in both resistance sites. Restriction enzyme analysis of total plastid DNA (ptDNA), and the recloning and full sequencing of the fragment introduced, investigated in one of the plastid transformants, showed no DNA rearrangements accompanied with the integration process. Sequence analysis indicated a targeted, homologous integration of the DNA fragment introduced but an unexpectedly complete homology of the parental ptDNA sequences in this region prevented the

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“…2. Comparison of the leucine repeat containing regions in the photosystem I reaction-centre polypeptides deduced from the psuA and psuB gene sequences from Synechococcus [17], Murchantiu [a], Chlamydomonas [18], tobacco [19], spinach [20], pea [213 and maize [22]. The conserved leucine repeats and iron-sulphur binding regions are in bold.…”

Section: Hypothesismentioning

confidence: 99%

Photosystem I reaction‐centre proteins contain leucine zipper motifs

Webber¹,

Malkin²

1990

FEBS Letters

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The photosystem I (PS I) reaction-centre polypeptides, encoded by the psaA and psaB genes, are shown to contain several highly conserved leucine repeats, consisting of a leucine residue every seventh amino acid, similar to the leucine zipper motifs known to mediate DNA-binding polypeptide dimerisation. In each of the PSI reaction-centre subunits the leucine zipper motif precedes highly conserved cysteine residues which have been proposed to ligate the interpolypeptide [4Fe-4S] centre, Fx. We propose that PS I reaction-centre dimerisation and [4Fe-4S] centre formation are mediated through the leucine zipper.

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The complete nucleotide sequence of the tobacco chloroplast genome

Cited by 147 publications

References 1 publication

N‐terminal sequence analysis of the 8 kDa protein inChlamydomonas reinhardiiLocalization of the phosphothreonine

N‐terminal sequence analysis of the 8 kDa protein inChlamydomonas reinhardiiLocalization of the phosphothreonine

Chloroplast transformation in plants: polyethylene glycol (PEG) treatment of protoplasts is an alternative to biolistic delivery systems

Photosystem I reaction‐centre proteins contain leucine zipper motifs

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