The Complete Sequence of the Mitochondrial Genome of the Honeybee Ectoparasite Mite Varroa destructor (Acari: Mesostigmata)

Navajas, María; Conte, Yves Le; Solignac, Michel; Cros‐Arteil, Sandrine; Cornuet, Jean‐Marie

doi:10.1093/oxfordjournals.molbev.a004055

Cited by 85 publications

(79 citation statements)

References 25 publications

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“…DQ059790]. It also showed 76.7% similarity with a CO-I gene sequence of Varroa destructor submitted by Navajas et al (2002) [VDE493124] and 77.3% similarity with a CO-I gene sequence obtained in an unpublished study by the senior author from V. jacobsoni [EF025469]. These Varroa sequences diVered by 6.6% and were used as outgroup in our phylogenetic analyses.…”

Section: Analysis Of Co-i Gene Sequencesmentioning

confidence: 68%

Genetic and morphological variation of bee-parasitic Tropilaelaps mites (Acari: Laelapidae): new and re-defined species

2007

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Mites in the genus Tropilaelaps are parasites of social honeybees. Two species, Tropilaelaps clareae and T. koenigerum, have been recorded and their primary hosts are presumed to be the giant honeybees of Asia, Apis dorsata and A. laboriosa. The most common species, T. clareae, is also an economically important pest of the introduced Western honeybee (A. mellifera) throughout Asia and is considered an emerging threat to world apiculture. In the studies reported here, genetic (mtDNA CO-I and nuclear ITS1-5.8S-ITS2 gene sequence) and morphological variation and host associations were examined among Tropilaelaps isolates collected from A. dorsata, A. laboriosa and A. mellifera throughout Asia and neighbouring regions. The results clearly indicate that the genus contains at least four species. Tropilaelaps clareae, previously assumed to be ubiquitous in Asia, was found to be two species, and it is here redefined as encompassing haplotypes (mites with distinct mtDNA gene sequences) that parasitise native A. dorsata breviligula and introduced A. mellifera in the Philippines and also native A. d. binghami on Sulawesi Island in Indonesia. Tropilaelaps mercedesae n. sp., which until now has been mistaken for T. clareae, encompasses haplotypes that, together with haplotypes of T. koenigerum, parasitise native A. d. dorsata in mainland Asia and Indonesia (except Sulawesi Island). It also parasitises introduced A. mellifera in these and surrounding regions and, with another new species, T. thaii n. sp., also parasitises A. laboriosa in mountainous Himalayan regions. Methods are described for identifying each species. These studies help to clarify the emerging threat of Tropilaelaps to world apiculture and will necessitate a revision of quarantine protocols for countries that import and export honeybees.

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Section: Analysis Of Co-i Gene Sequencesmentioning

confidence: 68%

Genetic and morphological variation of bee-parasitic Tropilaelaps mites (Acari: Laelapidae): new and re-defined species

2007

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“…Swarming activity did not seem to play a major role (Fries et al, 2003), as there was no marked difference between VSB and control bees in our study. Further, it seems unlikely that more benign mites strains have been selected, as after the development of molecular markers (Navajas et al, 2002;Solignac et al, 2003) we concluded that the Korean haplotype representing the Varroa mite population in Europe is a single invasive clone (Solignac et al, 2005), thus probably showing little genetic variation.…”

Section: Discussionmentioning

confidence: 88%

Honey bee colonies that have survivedVarroa destructor

Conte¹,

Vaublanc²,

Crauser³

et al. 2007

Apidologie

158

129

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-We document the ability of a population of honey bee colonies to survive in France without Varroa suppression measures. We compared the mortality of collected Varroa surviving bee (VSB) stock with that of miticide-treated Varroa-susceptible colonies. Varroa infestation did not induce mortality in the VSB colonies. Some of the original colonies survived more than 11 years without treatment and the average survival of the experimental colonies was 6.54 ± 0.25 years. Swarming was variable (41.50 ± 9.94%) depending on the year. Honey production was significantly higher (1.7 times) in treated than in VSB colonies. For the first time since Varroa invaded France, our results provide evidence that untreated local honey bee colonies can survive the mite, which may be the basis for integrated Varroa management.Apis mellifera / honey bee / Varroa destructor / host-parasite relationship / natural selection / tolerance

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“…The sequences of the six fragments spanning 5185 bp proved to be completely identical within each of the K and J types (table 2). Sequences of the K type are identical to the previously published complete sequence of the mitochondrial genome (Varroa from Avignon, France; EMBL accession number AJ493124; Navajas et al (2002)). EMBL accession numbers for the sequences of the J haplotype are: (accession number: AJ784872).…”

Section: (V) Double Infestation Of Cells By Varroa Foundressesmentioning

confidence: 83%

The invasive Korea and Japan types ofVarroa destructor, ectoparasitic mites of the Western honeybee (Apis mellifera), are two partly isolated clones

et al. 2005

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Varroa destructor , now a major pest of the Western honeybee, Apis mellifera , switched from its original host, the Eastern honeybee, A. cerana , ca . 50 years ago. So far, only two out of several known mitochondrial haplotypes of V. destructor have been found to be capable of reproducing on A. mellifera (Korea and Japan). These haplotypes are associated in almost complete cytonuclear disequilibrium to diagnostic alleles at 11 microsatellite loci. By contrast, microsatellite polymorphism within each type is virtually absent, because of a severe bottleneck at the time of host change. Accordingly, 12 mitochondrial sequences of 5185 nucleotides displayed 0.40% of nucleotide divergence between haplotypes and no intra haplotype variation. Hence, each type has a quasi–clonal structure. The nascent intratype variability is subsequent to the clone formation 50 years ago: in both types the variant alleles differ from the most common by one (in 10 cases), two (five cases) or three (one case) repeated motifs. In addition to individuals of the two ‘pure’ types, five F 1 hybrids and 19 recombinant individuals (Japan alleles introgressed into the Korea genetic background) were detected. The existence of F 1 and recombinant individuals in admixed populations requires that double infestations of honeybee cells occur in a high proportion but the persistence of pure types suggests a post–zygotic isolation between the two clones.

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The Complete Sequence of the Mitochondrial Genome of the Honeybee Ectoparasite Mite Varroa destructor (Acari: Mesostigmata)

Cited by 85 publications

References 25 publications

Genetic and morphological variation of bee-parasitic Tropilaelaps mites (Acari: Laelapidae): new and re-defined species

Genetic and morphological variation of bee-parasitic Tropilaelaps mites (Acari: Laelapidae): new and re-defined species

Honey bee colonies that have survivedVarroa destructor

The invasive Korea and Japan types ofVarroa destructor, ectoparasitic mites of the Western honeybee (Apis mellifera), are two partly isolated clones

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