The concordance between colposcopic biopsy and loop electrosurgical excision procedures (LEEP) in patients with known smear cytology and human papilloma virus results.

Gezer, Şener

doi:10.14744/nci.2021.80090

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…A LLETZ procedure covers the surgical excision of the transformation zone on the cervix by a loop to prevent tumor development for patients with CDB-confirmed CIN2+ diagnosis [ 37 , 38 ]. This technique removes a relatively large tissue part from the cervix and is considered the gold standard with utmost diagnostic accuracy [ 35 , 39 , 40 ] through histopathological analysis of the removed tissue. Considering this, we plotted the samples from the patients selected for the LLETZ procedure separately.…”

Section: Resultsmentioning

confidence: 99%

“…Two patients were diagnosed with CIN1 and CIN2, respectively, by CBD. However, the CDB method has been reported to have only a 60% consistency with the LLETZ histopathology result [ 34 , 35 , 40 , 46 ]. Additionally, despite abnormal cytology reports, two patients were not recruited for CDB (annotated as ‘no CDB procedure’).…”

Section: Discussionmentioning

confidence: 99%

“…The ThinPrep cell fixation solution, which contains 35–55% methanol, serves as a long-term stabilizer for biological material [ 40 , 46 , 47 , 54 ]. The samples were stored at room temperature after collection.…”

Section: Methodsmentioning

confidence: 99%

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Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer

Kose

Laar

Beekhuizen

et al. 2023

IJMS

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Triage methods for cervical cancer detection show moderate accuracy and present considerable false-negative and false-positive result rates. A complementary diagnostic parameter could help improve the accuracy of identifying patients who need treatment. A pilot study was performed using a targeted proteomics approach with opportunistic ThinPrep samples obtained from women collected at the hospital’s outpatient clinic to determine the concentration levels of minichromosome maintenance-3 (MCM3) and envoplakin (EVPL) proteins. Forty samples with ’negative for intraepithelial lesion or malignancy’ (NILM), 21 samples with ‘atypical squamous cells of undetermined significance’ (ASC-US), and 33 samples with ‘low-grade squamous intraepithelial lesion and worse’ (≥LSIL) were analyzed, using cytology and the patients’ histology reports. Highly accurate concordance was obtained for gold-standard-confirmed samples, demonstrating that the MCM3/EVPL ratio can discriminate between non-dysplastic and dysplastic samples. On that account, we propose that MCM3 and EVPL are promising candidate protein biomarkers for population-based cervical cancer screening.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer

Kose

Laar

Beekhuizen

et al. 2023

IJMS

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show abstract

“…[ 4 ] When Cervical Intraepithelial Neoplasia (CIN)2/3 is detected in CDB but there is still high clinical suspicion of cancer, then to rule out invasive malignancy, LEEP may be necessary. [ 5 ]…”

Section: Introductionmentioning

confidence: 99%

Cervical Cytology and Histology Correlation as an Analytic Quality Assurance Exercise: Experience from an Accredited Cytology Laboratory

Belekar,

Kamal,

Warke

2023

Journal of Cytology

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Context: The performance parameters of cervical cytology in any accredited cytology laboratory requires implementation of quality control exercise, which ensures acceptable performance by a laboratory. This study aims to assess the analytical aspect of quality control measures by evaluating the frequency and accuracy of epithelial abnormalities as detected in cervical Pap smears using histopathologic diagnosis as the gold standard. Methods: A retrospective diagnostic test study from 2018 to 2020 was conducted. Out of the total 6000 Pap smears, histopathologic correlation was available in 150 cases in the form of colposcopic-directed biopsy (CDB) and loop electrosurgical excision procedure (LEEP) tissue in 105 cases. The quality control measures analyzed were Atypical Squamous Cell: Squamous Intraepithelial Lesion (ASC: SIL) ratio, cyto-histo correlation, and study parameters like sensitivity, specificity, positive predictive value, and negative predictive value of Pap smears as against CDB and LEEP. Results: 4.5% smears were reported as inadequate, 92.3% as Negative for intraepithelial lesion or malignancy (NILM), followed by epithelial abnormality found in 3.21%. The ASC: SIL ratio was 1.3:1. Concordance rate against CDB was 100% in Squamous cell carcinoma (SCC), 82.35% in high-grade squamous intraepithelial lesion (HSIL), 82% in atypical squamous cells of undetermined significance (ASCUS), 65.6% in low-grade squamous intraepithelial lesion (LSIL), and 50% in Atypical Squamous Cell ,High grade Squamous Intraepithelial Lesion can not be ruled out (ASC-H). Total concordance rate was 84.15%. Sensitivity of Pap smear was 65% for LSIL and 82% for HSIL. Specificity, positive predictive value, and negative predictive value were 63.63%, 90%, and 75%, respectively. Concordance rate was 96% with LEEP. Conclusion: Quality control measures give an insight of performance of any accredited cytology laboratory. This exercise needs to be conducted on a regular basis, so that relevant steps can be taken in case of major discrepancy.

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Comparison of Three Methods, “Pap Smear, Conization and LEEP” in Women with Abnormal Pap Smear: A Ten-Year Retrospective Analytical Study

Taghipour Zahir,

Yazdkhasti,

Ghasempour

et al. 2023

Euras J Fam Med

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Aim: Considering that many cases of abnormal pap smears are reported, which lead to more aggressive procedures such as colposcopy and cervical Loop Electrosurgical Excision Procedure conization, therefore, we tried to investigate the results of conization and compare them with the results of pap smears, so that perhaps more aggressive procedures can be avoided. Methods: This cross-sectional retrospective analytical study was conducted on women who underwent pap smear and cervical conization in Mortaz and Shahid Sadoughi hospitals from 2011 to 2020. All data including age, Pap smear, and conization pathology results were extracted from medical records. P-value Results: Totally 1306 patients were included in this study. The mean age was 40.74±9.73 years old. The most commonly reported pap smear tests and loop electrosurgical excision procedure conization were atypical squamous cells of undetermined significance with 912 (69.8%) patients for a pap smear and chronic cervicitis with 860 (65.8%) patients for loop electrosurgical excision procedure, respectively. A significant difference was seen between the distribution of conization findings and the pap smear test. In addition, no significant difference was observed between the distribution of findings of pap smear tests and cervical conization in terms of age. Conclusion: According to the results, it can be said that there was no significant difference between different age groups, but there is a significant difference between the results of pap smear and cervical conization, it seems that pap smears should be reported more accurately to avoid unnecessary conization. Keywords: uterine cervical neoplasms, papanicolaou test, conization, cervix uteri

show abstract

The concordance between colposcopic biopsy and loop electrosurgical excision procedures (LEEP) in patients with known smear cytology and human papilloma virus results.

Cited by 3 publications

References 33 publications

Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer

Quantitative Proteomic Analysis of MCM3 in ThinPrep Samples of Patients with Cervical Preinvasive Cancer

Cervical Cytology and Histology Correlation as an Analytic Quality Assurance Exercise: Experience from an Accredited Cytology Laboratory

Comparison of Three Methods, “Pap Smear, Conization and LEEP” in Women with Abnormal Pap Smear: A Ten-Year Retrospective Analytical Study

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