The conserved RNA-binding protein Imp is required for the specification and function of olfactory navigation circuitry in Drosophila

Hamid, Aisha; Gattuso, Hannah; Caglar, Aysu Nora; Pillai, Midhula; Steele, Theresa; Gonzalez, Alexa; Nagel, Katherine; Syed, Mubarak Hussain

doi:10.1016/j.cub.2023.12.020

Cited by 9 publications

(1 citation statement)

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“…Imp and Syp levels are known to influence specification of the mushroom body (Liu et al, 2020, 2015), motor neurons (Guan et al, 2022), the central complex (Hamid et al, 2023), and the visual system (Arain et al, 2022). Beyond fate patterning, they also regulate NB quiescence exit (Munroe et al, 2022), NB growth and self-renewal (Hailstone et al, 2020; Landskron et al, 2018; Samuels et al, 2020b), NB decommissioning (Pahl et al, 2019; C. P. Yang et al, 2017), synaptic transmission (Boylan et al, 2008; Halstead et al, 2014; Hansen et al, 2015; Medioni et al, 2014; Titlow et al, 2020), and fly behaviour (Hamid et al, 2023; Samuels et al, 2020a). Furthermore, the protracted nature of Imp and Syp gradients allows integration of extrinsic signals into the intrinsic patterning programme (e.g.…”

Section: Introductionmentioning

confidence: 99%

Imp and Sypin vivotemporal RNA interactomes uncover networks of temporal regulators ofDrosophilabrain development

Lee,

Huang,

Samuels

et al. 2024

Preprint

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Temporal patterning of neural progenitors is an evolutionarily conserved mechanism generating neural diversity. InDrosophila, post-embryonic neurogenesis requires the RNA-binding proteins (RBPs) Imp/IGF2BP and Syp/SYNCRIP. However, how they co-achieve their function is not well understood. Here, we elucidate thein vivotemporal RNA interactome landscapes of Imp and Syp during larval brain development. Imp and Syp bind a highly overlapping set of conserved mRNAs encoding proteins involved in neurodevelopment. We identify transcripts differentially occupied by Imp/Syp over time, featuring a network of known and novel candidate temporal regulators that are post-transcriptionally regulated by Imp/Syp. Furthermore, the physical and co-evolutionary relationships between Imp and Syp binding sites reveal a combinatorial, rather than competitive, mode of molecular interplay. Our study establishes a newin vivoframework for dissecting the temporal co-regulation of RBP networks as well as providing a resource for understanding neural fate specification.

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