The constitution of functional model rabbit bladder with acellular matrix

Abstract

In this study, for the useable of bladder acelluar matrix in urinary tissue engineering, we compared the urodynamic and histopathological results between normal, partial cystectomy and bladder acellular matrix allograft (BAMA) augmented cystoplasty groups in rabbit model.Material and methods: There were five groups each consisting of three rabbits. Urodynamic investigation was applied to all animals at the beginning of the study. Then, in the first control group, the rabbits were sacrified and the bladders were removed for histopathological examination. In the second control group the same procedures were realized at the end of 24th week. In the third group, partial cystectomy was performed to the animals and followed for 24 weeks. Partial cystectomy and BAMA augmented cystoplasty were performed to the animals in the 4th and 5th groups. The animals were sacrified at the end of 12th and 24th weeks, respectively. In the 3th, 4th and 5th groups urodynamic investigations were performed before sacrification. Then bladders underwent histopathological examination.Results: There was no significant difference in term of bladder capacity between both control and BAMA augmented groups. Detrusor presures were no statistical different in all groups. However bladder capacity has significantly reduced in partial cystectomy group. In histopathological evaluations there were no differences between control and partial cystectomy groups. In BAMA augmented groups urothelium and vascularization were nearly the same as control groups however muscle layer was less organized and was thinner than control groups. Collagen depositon and innervation in BAMA augmented bladder were more prominent than that of the control group. Conclusion:In our animal study, although the detrusor muscle had less organized with more collagen deposition, the BAMA augmented bladders had near normal capacity and compliance with normal detrusor presures.