The contribution of CMP kinase to the efficiency of DNA repair

Tsao, Ning; Lee, Ming-Hsiang; Zhang, Wei; Cheng, Yung‐Chi; Chang, Zee-Fen

doi:10.4161/15384101.2014.987618

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…A knockdown of CMPK remarkably delays DNA repair during recovery from UV damage (Tsao et al . ). Intriguingly, CMPK1 has been identified as being under selection in Egyptian sheep populations adapted to hot dryland (Mwacharo ).…”

Section: Resultsmentioning

confidence: 97%

Genome‐wide scan reveals divergent selection among taurine and zebu cattle populations from different regions

Edea

Dadi

Dessie³

et al. 2018

Animal Genetics

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In this study, to identify genomic signatures of divergent selection, we genotyped 10 cattle breeds/populations (n = 275), representing eight Ethiopian cattle populations (n = 229) and two zebu populations (n = 46) adapted to tropical and sub-tropical environments, using the high-density single-nucleotide polymorphisms (SNPs) derived mainly from Bos indicus breeds, and using five reference taurine breeds (n = 212). Population genetic differentiation (F ST ) values across sliding windows were estimated between zebu and reference combined taurine breeds. The most differentiated regions (F ST ≥ 0.53), representing the top 1% smoothed F ST values, were considered to represent regions under diversifying selection. In total, 285 and 317 genes were identified in the comparisons of Ethiopian cattle with taurine and Asian zebu with taurine respectively. Some of these genes are involved in stress responses/thermo-tolerance and DNA damage repair (HSPA4, HSF1, CMPK1 and EIF2AK4), pigmentation (ERBB3 and MYO1A), reproduction/fertility (UBE2D3, ID3 and PSPC1), immune response (PIK3CD and AKIRIN2) and body stature and size (MBP2, LYN and NPM1). Additionally, the candidate genes were associated with functional terms (e.g. cellular response to stress, DNA repair, inflammatory response) important for physiological adaptation to environmental stresses. The results of our study may shed light on the influence of artificial and natural selection in shaping the genomic diversity of modern cattle breeds and also may serve as a basis for further genetic investigation of traits of tropical adaptation in cattle.

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Section: Resultsmentioning

confidence: 97%

Genome‐wide scan reveals divergent selection among taurine and zebu cattle populations from different regions

Edea

Dadi

Dessie³

et al. 2018

Animal Genetics

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“…PMS1 which belongs to the mutL/hexB family of DNA mismatch repair proteins, also forms heterodimers with MLH1 , a DNA mismatch repair protein 42 . Knockdown of CMPK1 was observed to delay DNA repair during recovery from UV damage, suggesting it contributes to the efficiency of the DNA repair process 43 . This finding could be associated with the fact that long term exposure to acute and chronic heat stress enhances the production of free radicals which can result in DNA damage and induce oxidative stress leading to mitochondria damage 44 – 46 , apoptosis and necrosis 47 , 48 .…”

Section: Discussionmentioning

confidence: 99%

Genomic footprints of dryland stress adaptation in Egyptian fat-tail sheep and their divergence from East African and western Asia cohorts

Mwacharo¹,

Kim

Elbeltagy

et al. 2017

Sci Rep

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African indigenous sheep are classified as fat-tail, thin-tail and fat-rump hair sheep. The fat-tail are well adapted to dryland environments, but little is known on their genome profiles. We analyzed patterns of genomic variation by genotyping, with the Ovine SNP50K microarray, 394 individuals from five populations of fat-tail sheep from a desert environment in Egypt. Comparative inferences with other East African and western Asia fat-tail and European sheep, reveal at least two phylogeographically distinct genepools of fat-tail sheep in Africa that differ from the European genepool, suggesting separate evolutionary and breeding history. We identified 24 candidate selection sweep regions, spanning 172 potentially novel and known genes, which are enriched with genes underpinning dryland adaptation physiology. In particular, we found selection sweeps spanning genes and/or pathways associated with metabolism; response to stress, ultraviolet radiation, oxidative stress and DNA damage repair; activation of immune response; regulation of reproduction, organ function and development, body size and morphology, skin and hair pigmentation, and keratinization. Our findings provide insights on the complexity of genome architecture regarding dryland stress adaptation in the fat-tail sheep and showcase the indigenous stocks as appropriate genotypes for adaptation planning to sustain livestock production and human livelihoods, under future climates.

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“…Tip60-mediated interaction is critical for recruitment of a number of nucleotide synthesis enzymes to DNA lesion sites (9)(10)(11). We then tested the possibilities of TMPK complex with Tip60 and the role of ATM activation for complex formation.…”

Section: Atm-dependent Phosphorylation Of Tmpk and Tip60 Complex Formmentioning

confidence: 99%

“…This mechanism is important in the local synthesis of dNTPs for DNA repair in quiescent cells that contain low levels of total dNTPs (9). We have previously shown that CMPK, a cytidylate kinase, and nucleoside diphosphate kinase 3, a nucleotide diphosphate kinase, also form a complex with Tip60-RNR for facilitating DNA repair (10,11). On the other hand, our previous study showed that TMPK is detected at double-strand break (DSB) sites (12).…”

mentioning

confidence: 99%

Thymidylate kinase is critical for DNA repair via ATM‐dependent Tip60 complex formation

Tsao

Wang

et al. 2018

FASEB j.

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Cellular supply of deoxythymidine triphosphate (dTTP) is crucial for DNA replication and repair. Thymidylate kinase (TMPK) catalyzes the conversion of thymidine monophosphate to thymidine diphosphate, which is an essential step for dTTP synthesis. Despite their major cellular localization in cytosol, TMPK and ribonucleotide reductase (RNR) are detected at DNA damage sites for local dNDP formation. Because deoxyuridine diphosphate is synthesized by RNR, the simultaneous recruitment of TMPK and RNR to DNA damage sites is critical for preventing deoxyuridine triphosphate-mediated toxic repair. This study investigates the mechanism responsible for the recruitment of TMPK to DNA damage sites. Our data demonstrate the requirement of ataxia telangiectasia mutated (ATM) kinase activity for TMPK recruitment to DNA lesion sites. Moreover, we find that TMPK is able to form the complex with histone acetyltransferase Tip60 and RNR. Inhibition of ATM kinase reduces the complex formation and TMPK phosphorylation. Our analysis further shows the presence of TMPK phosphorylation at serine 88, which is an ATM kinase consensus site. A phosphorylation-defective mutation at this site suppresses TMPK recruitment to DNA damage sites and the complex formation with Tip60. Finally, we provide evidence that this site is critical for the function of TMPK in DNA repair but not for catalytic activity. Together, these findings suggest that Tip60-ATM signaling has a functional contribution to the recruitment of TMPK to DNA damage sites, thereby increasing local dTTP synthesis for DNA repair.-Hu, C.-M., Tsao, N., Wang, Y.-T., Chen, Y.-J., Chang, Z.-F. Thymidylate kinase is critical for DNA repair via ATM-dependent Tip60 complex formation.

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The contribution of CMP kinase to the efficiency of DNA repair

Cited by 16 publications

References 33 publications

Genome‐wide scan reveals divergent selection among taurine and zebu cattle populations from different regions

Genome‐wide scan reveals divergent selection among taurine and zebu cattle populations from different regions

Genomic footprints of dryland stress adaptation in Egyptian fat-tail sheep and their divergence from East African and western Asia cohorts

Thymidylate kinase is critical for DNA repair via ATM‐dependent Tip60 complex formation

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