The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium

Canessa, Paulo; Álvarez, José M.; Polanco, Rubén; Bull, Paulina; Vicuña, Rafael

doi:10.1099/mic.0.2007/013128-0

Cited by 21 publications

(30 citation statements)

References 35 publications

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“…In P. chrysosporium, ACE elements in the promoter region of MCO genes have been shown to mediate their induction by copper. Only mco1, which contains ACE elements in its promoter, could be upregulated by copper (Canessa et al, 2008). Our results were consistent with these reports.…”

Section: Discussionsupporting

confidence: 95%

Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698

Wei

Xiao

2015

J Microbiol.

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One of the laccase isoforms in the brown rot fungus Postia placenta is thought to contribute to the production of hydroxyl radicals, which play an important role in lignocellulose degradation. However, the presence of at least two laccase isoforms in this fungus makes it difficult to understand the details of this mechanism. In this study, we systematically investigated the transcriptional patterns of two laccase genes, Pplcc1 and Pplcc2, by quantitative PCR (qPCR) to better understand the mechanism. The qPCR results showed that neither of the two genes was expressed constitutively throughout growth in liquid culture or during the degradation of a woody substrate. Transcription of Pplcc1 was upregulated under nitrogen depletion and in response to a high concentration of copper in liquid culture, and during the initial colonization of intact aspen wafer. However, it was subject to catabolite repression by a high concentration of glucose. Transcription of Pplcc2 was upregulated by stresses caused by ferulic acid, 2, 6-dimethylbenzoic acid, and ethanol, and under osmotic stress in liquid culture. However, the transcription of Pplcc2 was downregulated upon contact with the woody substrate in solid culture. These results indicate that Pplcc1 and Pplcc2 are differentially regulated in liquid and solid cultures. Pplcc1 seems to play the major role in producing hydroxyl radicals and Pplcc2 in the stress response during the degradation of a woody substrate.

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Section: Discussionsupporting

confidence: 95%

Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698

Wei

Xiao

2015

J Microbiol.

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“…In particular, copper ions were revealed to regulate genes transcription in T. versicolor, Ceriporiopsis subvermispora, Pleurotus ostreatus, Pleurotus sajor-caju, and Trametes pubescens [18]. Many studies have marked differences in the transcriptional response to copper ions which exhibit different laccase genes within a single species, or gene families [19][20][21][22]. The fungus…”

Section: A N U S C R I P Tmentioning

confidence: 99%

The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction

et al. 2015

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“…This was demonstrated by complementation assays using a S. cerevisiae ace1Δ strain, in which the capacity of this mutant to grow at high copper concentration was restored when PcACE1 cDNA was introduced (Polanco et al, 2006). Moreover, in vitro transcribed and translated PcACE1 protein was able to bind in a gel-shift assay to the promoter of the yeast metallothionein in the presence of copper, but not in its absence (Canessa et al, 2008). The PcACE1 amino acid sequence contains the same three Cys motifs present at the amino terminus of Ace1, Amt1 and Crf1, and additional three.…”

Section: Introductionmentioning

confidence: 92%

The PcACE1 transcription factor from Phanerochaete chrysosporium contains a Cys- and Ser-rich transactivation domain

2011

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Transcription factor Ace1 from Saccharomyces cerevisiae regulates the expression of target genes when the copper concentration reaches 200 μM levels. We are studying the ortholog of Ace1 from fungus Phanerochaete chrysosporium PcACE1, isolated by complementation in yeast. In this report we show the localization of the transactivation region of PcACE1. Different PcACE1 fragments were ligated in frame to the GAL4 DNA-binding domain by site-directed mutagenesis in a suitable yeast expression vector. Transformation of an appropriate Saccharomyces cerevisiae strain was used as host. This strain contains the fusion GAL1:lacZ in its genome under the control of promoter sequences recognized by GAL4. Finally, we measured β-galactosidase activity in each yeast clone. The activation of the reporter gene is proportional to the transactivation capacity of the transcription factor PcACE1. The results obtained indicate that PcACE1 transactivation domain is located in the carboxy terminal half and contains an array of cysteines in the form of Cys-X-Cys and Cys-X 2 -Cys and a 60% of Ser. Therefore, these results show that this type of Cys motif can function as transcription activating domain not only in transcription factors that respond to minimal copper concentrations but also in those that respond to high copper concentrations. This is the fi rst transactivation domain reported in a basidiomycete fungus.

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The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium

Cited by 21 publications

References 35 publications

Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698

Transcriptional profiles of laccase genes in the brown rot fungus Postia placenta MAD-R-698

The Trametes hirsuta 072 laccase multigene family: Genes identification and transcriptional analysis under copper ions induction

The PcACE1 transcription factor from Phanerochaete chrysosporium contains a Cys- and Ser-rich transactivation domain

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