The core promoter controls basal and inducible expression of duck retinoic acid inducible gene-I (RIG-I)

Xiao, Yanna; Reeves, Matthew B.; Caulfield, Adam F.; Evseev, Danyel; Magor, Katharine E.

doi:10.1016/j.molimm.2018.09.002

Cited by 8 publications

(13 citation statements)

References 41 publications

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“…DuIRF7 upregulates type I IFNs but does not affect type II IFN expression . We showed that duIRF7 increases IFN-β signaling when overexpressed in DF-1 cells (Xiao et al, 2018). We also observed duIRF7 translocate to the nucleus upon stimulation with constitutively active RIG-I 2CARD.…”

Section: Irf7mentioning

confidence: 51%

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Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Campbell

Magor

2020

Front. Cell. Infect. Microbiol.

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Mallard ducks are a natural host and reservoir of avian Influenza A viruses. While most influenza strains can replicate in mallards, the virus typically does not cause substantial disease in this host. Mallards are often resistant to disease caused by highly pathogenic avian influenza viruses, while the same strains can cause severe infection in humans, chickens, and even other species of ducks, resulting in systemic spread of the virus and even death. The differences in influenza detection and antiviral effectors responsible for limiting damage in the mallards are largely unknown. Domestic mallards have an early and robust innate response to infection that seems to limit replication and clear highly pathogenic strains. The regulation and timing of the response to influenza also seems to circumvent damage done by a prolonged or dysregulated immune response. Rapid initiation of innate immune responses depends on viral recognition by pattern recognition receptors (PRRs) expressed in tissues where the virus replicates. RIG-like receptors (RLRs), Toll-like receptors (TLRs), and Nod-like receptors (NLRs) are all important influenza sensors in mammals during infection. Ducks utilize many of the same PRRs to detect influenza, namely RIG-I, TLR7, and TLR3 and their downstream adaptors. Ducks also express many of the same signal transduction proteins including TBK1, TRIF, and TRAF3. Some antiviral effectors expressed downstream of these signaling pathways inhibit influenza replication in ducks. In this review, we summarize the recent advances in our understanding of influenza recognition and response through duck PRRs and their adaptors. We compare basal tissue expression and regulation of these signaling components in birds, to better understand what contributes to influenza resistance in the duck.

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Section: Irf7mentioning

confidence: 51%

“…When chIRF7 is overexpressed in DF-1 cells, it caused increased cell death and resulted in higher levels of viral replication (Kim and Zhou, 2018). With transfection of mCherry-IRF7 (Xiao et al, 2018), we also observed increased cell death.…”

Section: Irf7mentioning

confidence: 66%

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Campbell

Magor

2020

Front. Cell. Infect. Microbiol.

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“…IRF3 is apparently missing in birds [131], and so avian MAVS is likely signaling through IRF7. Indeed, there is increased nuclear localization of chicken IRF7-mCherry in DF-1 cells upon stimulation of MAVS signaling [150]. Thus, RLR signaling in ducks mostly resembles the human paradigm.…”

Section: Influenza Virus Pattern Recognition—rig-i-like Receptorsmentioning

confidence: 99%

Innate Immune Responses to Avian Influenza Viruses in Ducks and Chickens

Evseev

Magor

2019

Veterinary Sciences

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Mallard ducks are important natural hosts of low pathogenic avian influenza (LPAI) viruses and many strains circulate in this reservoir and cause little harm. Some strains can be transmitted to other hosts, including chickens, and cause respiratory and systemic disease. Rarely, these highly pathogenic avian influenza (HPAI) viruses cause disease in mallards, while chickens are highly susceptible. The long co-evolution of mallard ducks with influenza viruses has undoubtedly fine-tuned many immunological host–pathogen interactions to confer resistance to disease, which are poorly understood. Here, we compare innate responses to different avian influenza viruses in ducks and chickens to reveal differences that point to potential mechanisms of disease resistance. Mallard ducks are permissive to LPAI replication in their intestinal tissues without overtly compromising their fitness. In contrast, the mallard response to HPAI infection reflects an immediate and robust induction of type I interferon and antiviral interferon stimulated genes, highlighting the importance of the RIG-I pathway. Ducks also appear to limit the duration of the response, particularly of pro-inflammatory cytokine expression. Chickens lack RIG-I, and some modulators of the signaling pathway and may be compromised in initiating an early interferon response, allowing more viral replication and consequent damage. We review current knowledge about innate response mediators to influenza infection in mallard ducks compared to chickens to gain insight into protective immune responses, and open questions for future research.

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“…Therefore, it has been suggested that the upregulation of the inserted RIG-I under recombinant chicken IFN-β stimulation was dependent on the ISRE motif in the Mx promoter. In ducks, the ISRE motif was also contained in the RIG-I promoter and affected the promoter activity [ 7 ]. Hence, in terms of IFN response, the expression pattern of RIG-I in knock-in DF-1 cells corresponds to that in duck cells.…”

Section: Discussionmentioning

confidence: 99%

“…Constant and high expression of duck RIG-I in transgenic chickens may lead to an inflammatory response in non-infected chickens. Therefore, Xiao and colleagues analyzed the expression activity of duck RIG-I promoters in DF-1 to address this problem [ 7 ]. Second, random integration of duck RIG-I into the chicken genome can cause host gene disruption and unstable expression.…”

Section: Introductionmentioning

confidence: 99%

Knock-in of the duck retinoic acid-inducible gene I (RIG-I) into the Mx gene in DF-1 cells enables both stable and immune response-dependent RIG-I expression

Ichikawa

Motoe

Ezaki

et al. 2021

Biochemistry and Biophysics Reports

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Waterfowls, such as ducks, are natural hosts of avian influenza virus (AIV) and can genetically limit the pathogenicity. On the other hand, some AIV strains cause severe pathogenicity in chickens. It is suggested that differences in the pathogenicity of AIV infection between waterfowls and chickens are related to the expression of retinoic acid-inducible gene I ( RIG-I ), a pattern recognition receptor that chickens evolutionally lack. Here, we knocked-in the duck RIG-I bearing the T2A peptide sequence at the 3′ region of the Mx , an interferon-stimulated gene (ISG), in chicken embryo fibroblast cells (DF-1) using the precise integration into target chromosome (PITCh) system to control the duck RIG-I expression in chickens. The expression patterns of the duck RIG-I were then analyzed using qPCR. The knocked-in DF-1 cells expressed RIG-I via the stimulation of IFN-β and poly(I:C) in a dose-dependent manner. Moreover, poly(I:C) stimulation in the knocked-in DF-1 cells upregulated RIG-I-like receptor (RLR) family signaling pathway-related genes IFN-β, OASL, and IRF7 . The IFN-β-dependent expression of RIG-I and upregulation of IFN-β in the poly(I:C) stimulation demonstrated a positive-feedback loop via RIG-I, usually evident in ducks. Overall, this novel strategy established RIG-I-dependent immune response in chickens without overexpression of RIG-I and disruption of the host genes.

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The core promoter controls basal and inducible expression of duck retinoic acid inducible gene-I (RIG-I)

Cited by 8 publications

References 41 publications

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Innate Immune Responses to Avian Influenza Viruses in Ducks and Chickens

Knock-in of the duck retinoic acid-inducible gene I (RIG-I) into the Mx gene in DF-1 cells enables both stable and immune response-dependent RIG-I expression

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