The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth

Yao, Qing; Xie, Tingting; Fu, Y.; Wan, Jiajia; Zhang, Wendie; Gao, Xuejun; Huang, Jing; Sun, Diangang; Zhang, Fuxian; Bei, Weicheng; Lei, Liancheng; Liu, Feng

doi:10.3389/fmicb.2022.1079390

Cited by 2 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TCSs´ discrimination in A. pleuropneumoniae Two-component systems in A. pleuropneumoniae (App) have not been thoroughly studied, though they show interesting features. Aside from QseC-QseB and NarQ-NarP systems being reported in response to catecholamines [17,87], the NarP's regulation target genes [88], and CpxA-CpxR contributing to bio lm formation and virulence [20,21,89,90] much is still unknown of the App's TCS network. Moreover, App is a pathogenic bacterium with a high prevalence that forms monospecies and multispecies bio lms [91][92][93][94][95] and achieves host colonization, establishment of infection, essential nutrients acquisition, metabolic adaptation, and persistence within the host, with just a limited set of environment sensory systems [2,17,19,[96][97][98][99][100].…”

Section: System-wide Discriminationmentioning

confidence: 99%

Two-component systems interface discrimination in Actinobacillus pleuropneumoniae

Martin,

Guerrero-Barrera,

Avelar-Gonzalez

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Pathogenic bacteria grow in different environments and have developed signaling systems known as two-component systems that allow them to thrive in distinct habitats efficiently. Actinobacillus pleuropneumoniae is an obligate pig pathogen that colonizes its host and survives outside it by forming biofilms. The small number of the two-component systems in this pathogen makes it a suitable model to assess the interaction specificity of these systems. Results This was done through multiple sequence alignments, mutual information, heterodimer modeling, structural data, molecular dynamics, and the interface coupling index, which were used to evaluate molecular recognition. For the study, more than two thousand homologue sequences were collected from a diverse range of bacteria. Four different clusters of specificity-determining residues were found for all evaluated systems. The system-wide discrimination capability of twocomponent systems relies on the composition of these clusters composed of 3, 3, 4, and 5 residue pairs, from systems CpxAR, NarQP, PhoRB, and QseCB, respectively. These residue pairs are spatially nearby, the shape and composition of each cluster are systemspecific and have minimal overlap among them. Conclusions The interaction interface composition of the twocomponent systems network in A. pleuropneumoniae was defined and their discriminatory components were described. In summary, molecular recognition depends on specific components from the interaction interface called orthologue interaction specificity clusters. These clusters enable the emergence of specificity, which allows the system to differentiate between cognate and non-cognate components, thereby enabling the system to recognize highly similar components through molecular recognition.

show abstract

Section: System-wide Discriminationmentioning

confidence: 99%

Two-component systems interface discrimination in Actinobacillus pleuropneumoniae

Martin,

Guerrero-Barrera,

Avelar-Gonzalez

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The CpxAR TCS is found in many bacteria, consisting of CpxA (histidine kinase) and CpxR (transcriptional regulatory protein), and has been implicated in envelope stress responses. In A. pleuropneumoniae , the CpxAR plays an important role in heat stress, cold stress, biofilm formation, capsule synthesis, and pathogenesis ( Li et al, 2018 ; Yan et al, 2020 ; Yao et al, 2022 ; Liu et al, 2022a , b ).…”

Section: Introductionmentioning

confidence: 99%

The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae

Wan,

Zhang,

Jia

et al. 2023

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

IntroductionActinobacillus pleuropneumoniae is an important respiratory pathogen, which can cause porcine contagious pleuropneumonia and lead to great economic losses to worldwide swine industry. High potassium is an adverse environment for bacteria, which is not conducive to providing turgor pressure for cell growth and division. Two-component system CpxAR is an important regulatory system of bacteria in response to environmental changes, which is involved in a variety of biological activities, such as antibiotic resistance, periplasmic protein folding, peptidoglycan metabolism and so on.MethodsHowever, little is known about the role of CpxAR in high potassium stress in A. pleuropneumoniae. Here, we showed that CpxAR is critical for cell division of A. pleuropneumoniae under high potassium (K+) stress.ResultsqRT-PCR analysis found that CpxAR positively regulated the cell division genes ftsEX. In addition, we also demonstrated that CpxR-P could directly bind the promoter region of the cell division gene ftsE by EMSA.DiscussionIn conclusion, our results described a mechanism where CpxAR adjusts A. pleuropneumoniae survival under high-K+ stress by upregulating the expression of the cell division proteins FtsE and FtsX. These findings are the first to directly demonstrate CpxAR-mediated high-K+ tolerance, and to investigate the detailed molecular mechanism.

show abstract

The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth

Cited by 2 publications

References 34 publications

Two-component systems interface discrimination in Actinobacillus pleuropneumoniae

Two-component systems interface discrimination in Actinobacillus pleuropneumoniae

The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae

Contact Info

Product

Resources

About